##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548026_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1290437
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80058383322859	31.0	31.0	31.0	30.0	33.0
2	31.13828261278931	31.0	31.0	33.0	30.0	34.0
3	31.297440324479226	31.0	31.0	34.0	30.0	34.0
4	35.16301067002883	35.0	35.0	37.0	33.0	37.0
5	34.980334568832106	35.0	35.0	37.0	32.0	37.0
6	35.006524146471314	36.0	35.0	37.0	32.0	37.0
7	35.476723776519115	35.0	35.0	37.0	35.0	37.0
8	35.56914673091364	37.0	35.0	37.0	35.0	37.0
9	37.38579101498175	39.0	37.0	39.0	35.0	39.0
10	36.47096216243025	38.0	35.0	39.0	32.0	39.0
11	36.06121492176681	38.0	35.0	39.0	31.0	39.0
12	35.141969735833676	37.0	34.0	39.0	30.0	39.0
13	34.62272625474936	37.0	33.0	39.0	27.0	39.0
14	35.59649405588959	38.0	33.0	40.0	27.0	41.0
15	35.966243993313896	38.0	34.0	40.0	30.0	41.0
16	36.17850929568821	38.0	34.0	40.0	31.0	41.0
17	36.1328503444957	38.0	34.0	40.0	30.0	41.0
18	36.068252847678735	37.0	34.0	40.0	30.0	41.0
19	35.98993596742809	37.0	34.0	40.0	30.0	41.0
20	35.83865000771057	37.0	34.0	40.0	30.0	41.0
21	35.681209543743705	37.0	34.0	40.0	29.0	41.0
22	35.51342839673692	37.0	34.0	40.0	29.0	41.0
23	35.50331244376905	36.0	34.0	40.0	29.0	41.0
24	35.370976653645236	36.0	34.0	40.0	29.0	41.0
25	35.21675292943398	36.0	34.0	40.0	29.0	41.0
26	34.94000559500386	36.0	34.0	40.0	27.0	41.0
27	35.00418540385931	36.0	33.0	40.0	27.0	41.0
28	35.05256823851145	36.0	34.0	40.0	27.0	41.0
29	35.10927925966165	36.0	34.0	40.0	28.0	41.0
30	35.053216855995295	36.0	34.0	40.0	27.0	41.0
31	34.906883482107226	36.0	33.0	40.0	27.0	41.0
32	34.71324365311906	36.0	33.0	40.0	27.0	41.0
33	34.561793407969546	36.0	33.0	40.0	26.0	41.0
34	34.442244758946	35.0	33.0	40.0	25.0	41.0
35	34.328480971949816	35.0	33.0	40.0	24.0	41.0
36	34.170101291268	35.0	33.0	40.0	24.0	41.0
37	34.0632266433774	35.0	33.0	40.0	23.0	41.0
38	34.024249924637935	35.0	33.0	40.0	23.0	41.0
39	34.052015712506694	35.0	33.0	40.0	23.0	41.0
40	33.84291677935459	35.0	33.0	40.0	23.0	41.0
41	33.84021459397088	35.0	33.0	40.0	23.0	41.0
42	33.79501052744148	35.0	33.0	40.0	23.0	41.0
43	33.72327823830222	35.0	33.0	40.0	23.0	41.0
44	33.53883839350546	35.0	32.0	39.0	23.0	41.0
45	33.394731397193354	35.0	32.0	39.0	23.0	41.0
46	33.30028199749387	35.0	32.0	39.0	23.0	41.0
47	33.26613000092217	35.0	32.0	39.0	23.0	41.0
48	33.205882968327785	35.0	32.0	39.0	23.0	41.0
49	33.07330384978112	35.0	32.0	39.0	22.0	41.0
50	32.908940149732224	35.0	31.0	39.0	21.0	40.0
51	32.53073648694202	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	15.0
15	62.0
16	156.0
17	407.0
18	897.0
19	1706.0
20	3083.0
21	4739.0
22	7364.0
23	10413.0
24	14755.0
25	19608.0
26	24659.0
27	28868.0
28	33996.0
29	39795.0
30	47309.0
31	57157.0
32	69192.0
33	87012.0
34	136419.0
35	161527.0
36	101441.0
37	116572.0
38	151032.0
39	172228.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.131546910077752	26.50807439650289	30.58026079537397	11.78011789804539
2	28.883548751314475	28.08536952985694	29.474201375193054	13.556880343635527
3	28.37333399460803	27.169865712157975	30.853269086363767	13.603531206870231
4	25.222540891186473	29.79448047444393	30.355453230184814	14.627525404184786
5	22.312828909896414	34.183536274920826	29.196775975890336	14.306858839292424
6	20.38921698618375	43.66575043957977	25.888594328897884	10.056438245338594
7	90.63883010174074	3.8151416923104344	4.516686982781802	1.0293412231670356
8	91.01637662280298	3.1338996014528413	4.132011093916247	1.717712681827939
9	86.75929161981561	5.800128173634203	5.467295187599239	1.9732850189509445
10	46.35259218388809	32.67040545179656	10.80843156233121	10.168570801984133
11	43.422267030471076	23.393703063380855	19.65969667639722	13.524333229750852
12	40.126251804621226	23.29861899496062	21.02280080313878	15.552328397279371
13	22.74299326507222	40.063947329470565	22.451231637034585	14.741827768422636
14	17.07677321713497	42.011969588596735	24.38398774988628	16.52726944438202
15	16.604530093293977	27.43985177114419	40.6703310583934	15.285287077168434
16	18.218479476332437	23.812088463055538	40.256207780775036	17.713224279836986
17	18.0423375957137	25.172867795948196	24.755799779454556	32.02899482888355
18	22.511288811464645	27.072456849888837	30.540041861787902	19.876212476858615
19	27.662334542484444	28.686173753542406	23.29156712028561	20.35992458368754
20	30.19899460415348	27.261540082933145	23.23422220534594	19.305243107567435
21	23.375569671359393	31.33085923605724	25.89192653341465	19.401644559168716
22	24.33338473710844	26.606025710670107	24.033718809984524	25.026870742236934
23	20.30366457254403	33.18046522224642	22.456501169758774	24.059369035450782
24	20.224621581681244	27.875983097198855	34.48878170728211	17.410613613837793
25	18.62307109917028	29.191893908807636	31.656175388647412	20.52885960337467
26	18.29775494658011	37.680568675572694	24.2899885852622	19.731687792584992
27	19.20240972631752	36.342029870501236	25.5489419475728	18.90661845560845
28	16.102219635673805	32.22497495034628	34.12138678602675	17.551418627953165
29	17.038414118628033	27.443493948174147	33.571030588862534	21.947061344335292
30	19.57236192080667	33.51833526162068	28.459738832659013	18.449563984913638
31	28.129540612986144	30.260291668636285	23.106436036784437	18.503731681593134
32	28.545058766913844	29.916609644639763	24.45822616679466	17.080105421651734
33	26.73822898754453	30.484944247568848	22.545773253556742	20.231053511329883
34	19.4143534322094	31.589918763953605	25.7727421020941	23.2229857017429
35	19.06989647692991	30.038583828579	28.384957963852553	22.506561730638534
36	29.06170545326893	28.12124884825838	24.394371829078057	18.422673869394632
37	20.3240452652861	33.84845598816525	27.46162734019561	18.36587140635304
38	19.468521128888895	35.52881698215411	23.085047933374508	21.91761395558249
39	20.1444936870223	33.597688224996645	25.783048688157578	20.47476939982347
40	23.54628703299735	29.90025859456913	23.98373574223306	22.569718630200466
41	18.107586809739644	27.862499292875203	26.980782479113664	27.049131418271482
42	21.55556606017961	28.41184807937156	24.19738429694747	25.835201563501357
43	22.139089316254882	28.955617360630548	25.33878058363175	23.566512739482825
44	20.088001196493902	32.26782865029443	26.014133196738783	21.630036956472885
45	18.14982056466143	38.00805463575517	21.573699452201076	22.26842534738232
46	22.004251273018365	34.42415243828253	23.9015155331101	19.670080755588998
47	21.1429151519989	30.118169271339863	26.130140409799164	22.608775166862078
48	22.029591525971433	28.247175181740758	29.073949367539832	20.649283924747973
49	21.94837872751634	26.48622133432318	29.069764738611802	22.49563519954868
50	20.165881790432234	33.50694377176104	26.02188250956846	20.305291928238265
51	18.378580279393724	34.000729985268556	23.559460864807814	24.061228870529906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1552.0
1	1521.5
2	1491.0
3	4214.5
4	6938.0
5	5508.5
6	4079.0
7	4272.0
8	4465.0
9	5269.5
10	6074.0
11	6585.5
12	7097.0
13	7301.5
14	7506.0
15	7072.0
16	6638.0
17	6444.0
18	6250.0
19	6473.0
20	6696.0
21	6832.0
22	6968.0
23	6785.5
24	6603.0
25	7659.0
26	9937.5
27	11160.0
28	14002.5
29	16845.0
30	19134.5
31	21424.0
32	24200.5
33	26977.0
34	31424.0
35	35871.0
36	37723.0
37	39575.0
38	47433.5
39	55292.0
40	71423.5
41	87555.0
42	102057.0
43	116559.0
44	120034.5
45	123510.0
46	124269.5
47	125029.0
48	119179.0
49	113329.0
50	108714.0
51	104099.0
52	93519.5
53	82940.0
54	73722.0
55	64504.0
56	57682.0
57	50860.0
58	44747.0
59	38634.0
60	33989.0
61	29344.0
62	25934.5
63	22525.0
64	18844.5
65	15164.0
66	12839.0
67	10514.0
68	8344.5
69	6175.0
70	5561.5
71	4948.0
72	3718.5
73	2489.0
74	2198.0
75	1530.5
76	1154.0
77	763.5
78	373.0
79	344.5
80	316.0
81	224.5
82	133.0
83	113.0
84	93.0
85	65.5
86	38.0
87	26.5
88	15.0
89	12.5
90	10.0
91	6.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1290437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.422266875703396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14050743673982	20.11931100735389
2	8.131345099578118	4.134344503598357
3	2.7600121313468895	2.1049729494983866
4	1.4653169383054534	1.4900671305235937
5	0.9153803039703688	1.1635521190148606
6	0.6244129430363564	0.952439548710818
7	0.46123362394680956	0.8207922996016529
8	0.39316322646634994	0.7996080375152127
9	0.3020805203317652	0.6911614445242933
>10	3.725432394343995	23.448922133441386
>50	1.698578078986588	30.593674813353385
>100	0.3800512766083776	12.805907126375448
>500	0.0015537664620106347	0.2351055812068428
>1k	9.322598772063808E-4	0.640141305281938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4348	0.3369401218346963	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2522	0.19543766956465133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03463942834869118	0.0
2	0.0	0.0	0.0	0.15095661392226045	0.0
3	0.0	0.0	0.0	0.23635404130538726	0.0
4	0.0	0.0	0.0	0.41536316767110676	0.0
5	0.0	0.0	0.0	0.7596651366940037	0.0
6	0.0	0.0	0.0	1.2152472379511747	0.0
7	0.0	0.0	0.0	1.4295157376919603	0.0
8	0.0	0.0	0.0	1.9491846560506247	0.0
9	0.0	0.0	0.0	2.128193782416344	0.0
10	0.0	0.0	0.0	2.4880718702269076	0.0
11	0.0	0.0	0.0	3.0434651207304193	0.0
12	0.0	0.0	0.0	3.4431746764855626	0.0
13	0.0	0.0	0.0	3.6476015489326485	0.0
14	0.0	0.0	0.0	3.7393534128361168	0.0
15	0.0	0.0	0.0	3.865279746318495	0.0
16	0.0	0.0	0.0	4.122634425392328	0.0
17	0.0	0.0	0.0	4.426872447085755	0.0
18	0.0	0.0	0.0	4.772104333648214	0.0
19	0.0	0.0	0.0	4.981103300664813	0.0
20	0.0	0.0	0.0	5.1715039168901695	0.0
21	0.0	0.0	0.0	5.466985215086052	0.0
22	0.0	0.0	0.0	5.766961114723152	0.0
23	0.0	0.0	0.0	6.102661346505099	0.0
24	0.0	0.0	0.0	6.356838807318761	0.0
25	0.0	0.0	0.0	6.578314167991153	0.0
26	0.0	0.0	0.0	6.789095476958581	0.0
27	0.0	0.0	0.0	6.9941422944320415	0.0
28	0.0	0.0	0.0	7.230573828865725	0.0
29	0.0	0.0	0.0	7.4695626365332055	0.0
30	0.0	0.0	0.0	7.7482279258886715	0.0
31	0.0	0.0	0.0	8.012711972765816	0.0
32	0.0	0.0	0.0	8.24984094535417	0.0
33	0.0	0.0	0.0	8.484955096606809	0.0
34	7.749312829684828E-5	0.0	0.0	8.731150765205895	0.0
35	7.749312829684828E-5	0.0	0.0	9.034148896846572	0.0
36	7.749312829684828E-5	0.0	0.0	9.320253526518536	0.0
37	7.749312829684828E-5	0.0	0.0	9.587837298527553	0.0
38	7.749312829684828E-5	0.0	0.0	9.85813333002696	0.0
39	7.749312829684828E-5	0.0	0.0	10.115255529715903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAG	95	0.0	45.000004	1
GGACGTA	35	1.2120836E-7	45.000004	8
TTACGTC	30	2.1657088E-6	45.000004	34
TAATCGT	130	0.0	45.000004	21
AGCCGAC	25	3.8910737E-5	45.0	12
TCGATGC	25	3.8910737E-5	45.0	35
ATATCGT	20	7.033609E-4	45.0	22
ATATCCG	20	7.033609E-4	45.0	1
CCCGTAC	25	3.8910737E-5	45.0	10
TCGTGCG	50	2.1827873E-11	45.0	1
CCGAGTA	25	3.8910737E-5	45.0	23
AGTTATC	25	3.8910737E-5	45.0	26
TTACGCC	20	7.033609E-4	45.0	12
GAACCGT	25	3.8910737E-5	45.0	16
TTGCACG	20	7.033609E-4	45.0	1
TACGTAG	45	3.8562575E-10	45.0	1
CGACGGT	220	0.0	43.97727	28
TACGGGA	475	0.0	43.105267	4
GACGGTC	225	0.0	43.0	29
CGCATCG	85	0.0	42.35294	21
>>END_MODULE