FastQCFastQC Report
Thu 26 May 2016
SRR1548025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548025_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1636568
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.23830357186502485No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20600.12587316872870544No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC253.8915332E-545.028
CGTACCG253.8915332E-545.029
CGGTCTA2950.043.47457531
CGGGTAT1400.043.392866
GACGTAG603.6379788E-1241.2500041
CGTCATA4600.040.59782438
CGACGGT3300.039.54545228
AGGGCGA13700.038.594896
GTTTGCG1050.038.571431
TAACGGG5000.037.83
CAGGCGT5000.037.834
TTAGGGA20550.037.6642344
GACCGAT7600.037.5986829
TACGGGA6050.037.5619854
CGTTTTT27950.037.5134161
AATGCGG2400.037.5000042
CCCGTAC301.1401331E-437.50000427
AGCGAAC301.1401331E-437.50000427
TCGATAG900.037.51
GGGCGAT25250.037.4257437