##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548025_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1636568
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.040036222142923	31.0	31.0	33.0	30.0	33.0
2	31.398634215015814	31.0	31.0	33.0	30.0	34.0
3	31.62014593955155	31.0	31.0	34.0	30.0	34.0
4	35.48580138436044	37.0	35.0	37.0	33.0	37.0
5	35.32711992413392	37.0	35.0	37.0	33.0	37.0
6	35.35617401782266	37.0	35.0	37.0	33.0	37.0
7	35.63236724657943	36.0	35.0	37.0	35.0	37.0
8	35.719166572974665	37.0	35.0	37.0	35.0	37.0
9	37.5813849470355	39.0	37.0	39.0	35.0	39.0
10	36.80806052666311	39.0	37.0	39.0	32.0	39.0
11	36.47205921171623	38.0	35.0	39.0	32.0	39.0
12	35.77105687023087	37.0	35.0	39.0	31.0	39.0
13	35.37837780037248	37.0	35.0	39.0	30.0	39.0
14	36.35851183696614	38.0	35.0	40.0	30.0	41.0
15	36.65425634620743	38.0	35.0	40.0	31.0	41.0
16	36.830626041814334	38.0	35.0	40.0	32.0	41.0
17	36.77890805637163	38.0	35.0	40.0	31.0	41.0
18	36.709826906061956	38.0	35.0	40.0	31.0	41.0
19	36.64976707353437	38.0	35.0	40.0	31.0	41.0
20	36.53638467818019	38.0	35.0	40.0	31.0	41.0
21	36.40216599615781	38.0	35.0	40.0	31.0	41.0
22	36.27780574959305	38.0	35.0	40.0	30.0	41.0
23	36.230644250651366	38.0	34.0	40.0	30.0	41.0
24	36.114764556070995	38.0	34.0	40.0	30.0	41.0
25	35.95319778952051	38.0	34.0	40.0	30.0	41.0
26	35.7457588074556	37.0	34.0	40.0	30.0	41.0
27	35.811181081384944	38.0	34.0	40.0	30.0	41.0
28	35.88469406709651	38.0	34.0	40.0	30.0	41.0
29	35.913492748239	38.0	34.0	40.0	30.0	41.0
30	35.86360542305605	38.0	34.0	40.0	30.0	41.0
31	35.77588404514814	38.0	34.0	40.0	30.0	41.0
32	35.64533462709768	38.0	34.0	40.0	29.0	41.0
33	35.53497196572339	37.0	34.0	40.0	29.0	41.0
34	35.42150830274086	37.0	34.0	40.0	28.0	41.0
35	35.28470983179434	37.0	34.0	40.0	27.0	41.0
36	35.176978897302156	37.0	34.0	40.0	27.0	41.0
37	35.10993188184054	37.0	34.0	40.0	27.0	41.0
38	35.045578918810584	37.0	34.0	40.0	27.0	41.0
39	35.037458266323185	37.0	34.0	40.0	27.0	41.0
40	34.828789271206574	37.0	34.0	40.0	26.0	41.0
41	34.76396886655489	37.0	34.0	40.0	25.0	41.0
42	34.73457076027394	37.0	33.0	40.0	26.0	41.0
43	34.70135368649515	37.0	33.0	40.0	26.0	41.0
44	34.516763128693704	36.0	33.0	40.0	25.0	41.0
45	34.38456269461459	36.0	33.0	40.0	24.0	41.0
46	34.36252511353027	36.0	33.0	40.0	24.0	41.0
47	34.315125922051514	36.0	33.0	40.0	24.0	41.0
48	34.23647413367486	36.0	33.0	40.0	24.0	41.0
49	34.13631697552439	36.0	33.0	40.0	24.0	41.0
50	33.99819378113222	35.0	33.0	40.0	24.0	41.0
51	33.64269984504158	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	7.0
14	37.0
15	61.0
16	152.0
17	369.0
18	796.0
19	1481.0
20	2548.0
21	3989.0
22	6153.0
23	9239.0
24	12974.0
25	17745.0
26	22957.0
27	27424.0
28	32554.0
29	39670.0
30	47928.0
31	60106.0
32	75241.0
33	98877.0
34	157441.0
35	189665.0
36	137597.0
37	172173.0
38	236558.0
39	282790.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.620906677877116	24.308186399831843	30.046903031221433	12.024003891069604
2	28.91887168758035	27.25545165248251	30.148090394044118	13.677586265893016
3	28.967998885472525	26.880276285495015	30.47713263365775	13.67459219537471
4	26.063567172277597	29.42065346505614	29.687797879464835	14.82798148320143
5	23.15180304148682	33.8836516417283	28.59160144888572	14.372943867899165
6	22.14060155153956	42.37599659775824	25.566245948839278	9.917155901862923
7	91.76758924774285	3.4177009449042144	3.785971618655626	1.0287381886973228
8	92.63825273376969	2.483856460593144	3.5565891548655477	1.3213016507716149
9	87.76683889700885	5.224225330080999	5.05399103489742	1.9549447380127194
10	43.11803725845794	34.33184566727444	11.724657942719153	10.82545913154846
11	40.30953800880867	23.59724741043452	21.16520670085203	14.928007879904776
12	37.947644094226455	21.73536327240909	23.607268381148845	16.70972425221561
13	24.80025272399314	32.5314316300942	24.57973026479804	18.088585381114626
14	19.3126713952613	35.86890370580385	26.391937273611603	18.426487625323237
15	18.0531453627347	26.361018912749117	38.45608615101847	17.129749573497712
16	21.67187675672505	22.761168494068073	37.3310488778957	18.235905871311182
17	22.439764189450116	22.970875637309295	26.05647916860161	28.532881004638977
18	23.79094544192481	25.420880770001613	30.68213480894164	20.10603897913194
19	28.58402461737001	25.94557635246443	25.17396160746147	20.2964374227041
20	30.12737631433585	26.508339402945673	24.42189997604744	18.942384306671034
21	25.926573170195187	26.76100229260257	27.964496434000907	19.347928103201333
22	25.50447033059427	23.717499059006407	26.744870973891704	24.033159636507616
23	22.387826231479536	28.45448523984338	25.798683586627625	23.35900494204946
24	22.298859564649927	25.77344784940192	32.851491658152916	19.076200927795238
25	21.203457479310362	26.282562044473558	31.463587214219025	21.05039326199706
26	20.98592909063357	31.93218980207361	25.384279785502343	21.697601321790476
27	19.780357430916407	32.79521535310479	27.902048677476284	19.522378538502526
28	18.284116517003877	29.53809435354962	32.80572515165884	19.37206397778766
29	19.338884788166453	26.850763304671727	32.212288154234955	21.59806375292686
30	21.223316110299116	29.327287347669024	29.65449648288369	19.79490005914817
31	26.989590411153095	27.674865938964956	25.535816415816516	19.799727234065433
32	28.29744929633232	26.70717012675306	26.36474622502701	18.630634351887608
33	27.034929193287415	27.94677642481095	25.01930869966906	19.998985682232576
34	21.485022314990886	28.213798632259707	27.76151067355588	22.539668379193532
35	21.706278015945564	27.069758176867687	29.2277497788054	21.996214028381345
36	28.386965894481627	25.946798422063733	26.495752086072805	19.17048359738184
37	22.450273988004167	30.729612212874745	27.490455636429406	19.329658162691683
38	22.690655078188012	30.219825879523494	25.15257538947358	21.936943652814918
39	22.305092119606396	29.458598726114648	27.02918546617067	21.207123688108283
40	25.141026831760122	26.30798109213916	26.061917378318526	22.48907469778219
41	20.102922701653707	25.321709822017784	28.426377639059297	26.14898983726921
42	22.593683855482936	26.116299475487725	26.282989768833314	25.00702690019602
43	22.873171172844636	26.047313646606803	27.128539724594397	23.95097545595417
44	21.9170239183462	28.811329562841266	27.625188809753094	21.646457709059447
45	20.33395495940285	32.22738071378642	24.73432206911048	22.70434225770026
46	22.69939287582306	29.96930161166539	26.230379672583116	21.100925839928436
47	22.82923777074952	27.824936085759955	27.018370150216796	22.32745599327373
48	22.859545096812354	26.348859320236006	29.309506234999095	21.48208934795254
49	22.492190975260424	25.421308494361373	29.90880916649965	22.177691363878555
50	21.71086077694297	29.610807494708443	27.24763040704694	21.43070132130165
51	20.23753366801746	31.323599141618313	25.768315157084825	22.6705520332794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1337.0
1	1653.0
2	1969.0
3	4660.5
4	7352.0
5	5796.0
6	4240.0
7	4139.5
8	4039.0
9	4555.0
10	5071.0
11	5235.0
12	5399.0
13	5417.5
14	5436.0
15	5315.5
16	5195.0
17	5014.0
18	4833.0
19	5468.0
20	6103.0
21	6305.5
22	6508.0
23	7005.5
24	7503.0
25	9507.5
26	13047.5
27	14583.0
28	16782.0
29	18981.0
30	23655.0
31	28329.0
32	34129.5
33	39930.0
34	45551.0
35	51172.0
36	52184.0
37	53196.0
38	60010.0
39	66824.0
40	81958.5
41	97093.0
42	114818.0
43	132543.0
44	135746.0
45	138949.0
46	136047.0
47	133145.0
48	129943.0
49	126741.0
50	123261.0
51	119781.0
52	112017.5
53	104254.0
54	95191.0
55	86128.0
56	82828.5
57	79529.0
58	75781.0
59	72033.0
60	65686.0
61	59339.0
62	53067.5
63	46796.0
64	39188.5
65	31581.0
66	26579.0
67	21577.0
68	18163.5
69	14750.0
70	11867.5
71	8985.0
72	7478.5
73	5972.0
74	4601.5
75	2890.0
76	2549.0
77	1689.5
78	830.0
79	711.5
80	593.0
81	471.0
82	349.0
83	258.5
84	168.0
85	128.0
86	88.0
87	62.5
88	37.0
89	22.5
90	8.0
91	7.0
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1636568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.164649785701588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.90008767481305	15.120925483489426
2	6.6410878814949035	2.545482468898334
3	2.1088078288434025	1.2124369051538864
4	1.0978895380690479	0.8416267400191597
5	0.7071732521110102	0.6776363857261584
6	0.49968073912047534	0.5745723821942657
7	0.3991959767591178	0.5355315763314693
8	0.3655687180575961	0.5604797163345371
9	0.29397580924896066	0.5070549086752091
>10	5.795651984588941	30.316194348579817
>50	2.8315326952641464	37.546969388746845
>100	0.35642108614099066	8.859199387823491
>500	0.002276412046515651	0.33044063338318524
>1k	6.504034418616145E-4	0.37144967464424444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3900	0.23830357186502485	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2060	0.12587316872870544	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02040856230844059	0.0
2	0.0	0.0	0.0	0.08413949191234339	0.0
3	0.0	0.0	0.0	0.12422337476963988	0.0
4	0.0	0.0	0.0	0.23029901598955863	0.0
5	0.0	0.0	0.0	0.3955839292959413	0.0
6	0.0	0.0	0.0	0.6093850056948443	0.0
7	0.0	0.0	0.0	0.7038509857213388	0.0
8	0.0	0.0	0.0	0.9180186830000342	0.0
9	0.0	0.0	0.0	0.9905485137189534	0.0
10	0.0	0.0	0.0	1.148195492029662	0.0
11	0.0	0.0	0.0	1.3906541005323336	0.0
12	0.0	0.0	0.0	1.5819690963039728	0.0
13	0.0	0.0	0.0	1.6674528647755547	0.0
14	0.0	0.0	0.0	1.710164197271363	0.0
15	0.0	0.0	0.0	1.7749949895146429	0.0
16	0.0	0.0	0.0	1.9182215465535193	0.0
17	0.0	0.0	0.0	2.0767239735837437	0.0
18	0.0	0.0	0.0	2.2675501415156596	0.0
19	0.0	0.0	0.0	2.3735035757756475	0.0
20	0.0	0.0	0.0	2.4903334294694752	0.0
21	0.0	0.0	0.0	2.646941648620772	0.0
22	0.0	0.0	0.0	2.816564909004698	0.0
23	0.0	0.0	0.0	2.9965757609827395	0.0
24	0.0	0.0	0.0	3.1301479681870843	0.0
25	0.0	0.0	0.0	3.252660445517693	0.0
26	0.0	0.0	0.0	3.3761505785277484	0.0
27	0.0	0.0	0.0	3.506423197813962	0.0
28	0.0	0.0	0.0	3.634068367461664	0.0
29	0.0	0.0	0.0	3.7789447184596057	0.0
30	0.0	0.0	0.0	3.9628661931554325	0.0
31	0.0	0.0	0.0	4.124973725503615	0.0
32	0.0	0.0	0.0	4.2708277321810035	0.0
33	0.0	0.0	0.0	4.420958982455969	0.0
34	0.0	0.0	0.0	4.588810241920898	0.0
35	0.0	0.0	0.0	4.787274344848488	0.0
36	0.0	0.0	0.0	4.959708365310822	0.0
37	0.0	0.0	0.0	5.139841424248794	0.0
38	0.0	0.0	0.0	5.327306900782613	0.0
39	0.0	0.0	0.0	5.611376979141716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACC	25	3.8915332E-5	45.0	28
CGTACCG	25	3.8915332E-5	45.0	29
CGGTCTA	295	0.0	43.474575	31
CGGGTAT	140	0.0	43.39286	6
GACGTAG	60	3.6379788E-12	41.250004	1
CGTCATA	460	0.0	40.597824	38
CGACGGT	330	0.0	39.545452	28
AGGGCGA	1370	0.0	38.59489	6
GTTTGCG	105	0.0	38.57143	1
TAACGGG	500	0.0	37.8	3
CAGGCGT	500	0.0	37.8	34
TTAGGGA	2055	0.0	37.664234	4
GACCGAT	760	0.0	37.598682	9
TACGGGA	605	0.0	37.561985	4
CGTTTTT	2795	0.0	37.513416	1
AATGCGG	240	0.0	37.500004	2
CCCGTAC	30	1.1401331E-4	37.500004	27
AGCGAAC	30	1.1401331E-4	37.500004	27
TCGATAG	90	0.0	37.5	1
GGGCGAT	2525	0.0	37.425743	7
>>END_MODULE