FastQCFastQC Report
Thu 26 May 2016
SRR1548024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548024_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1322918
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56750.428975945599047No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.27227689093352725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16950.1281258551172484No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC207.0336723E-445.00000444
CTAGACG207.0336723E-445.00000436
TCGTTGC207.0336723E-445.00000438
CACGGTC453.8562575E-1045.00000428
GTCGAAG800.045.0000041
GCGAATC207.0336723E-445.00000435
CGTGATA406.8157533E-945.00000439
CGTTGAT900.045.00000425
TAGCGGC207.0336723E-445.00000444
TCGATAG406.8157533E-945.0000041
ATGTACG453.8562575E-1045.0000041
CCCGTAG207.0336723E-445.00000429
TACGCGG900.045.0000042
TCTACCG207.0336723E-445.00000441
CTACGGA453.8562575E-1045.00000411
TTGCGCA207.0336723E-445.0000042
ACTAAGA207.0336723E-445.00000430
CAAGATT406.8157533E-945.00000426
ACTCCGA207.0336723E-445.00000439
TGTCGAA207.0336723E-445.00000412