##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548024_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1322918
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.901578933841705	31.0	31.0	33.0	30.0	33.0
2	31.23819541347234	31.0	31.0	33.0	30.0	34.0
3	31.406636692523648	31.0	31.0	34.0	30.0	34.0
4	35.24999508661912	37.0	35.0	37.0	33.0	37.0
5	35.061707528357765	35.0	35.0	37.0	32.0	37.0
6	35.10047863888767	37.0	35.0	37.0	32.0	37.0
7	35.51725050229871	35.0	35.0	37.0	35.0	37.0
8	35.610798250534046	37.0	35.0	37.0	35.0	37.0
9	37.45526858051671	39.0	37.0	39.0	35.0	39.0
10	36.634972084437585	39.0	37.0	39.0	32.0	39.0
11	36.14274127345762	38.0	35.0	39.0	31.0	39.0
12	34.702184111184515	35.0	33.0	39.0	30.0	39.0
13	33.73074068082829	35.0	33.0	39.0	23.0	39.0
14	34.679537204875885	35.0	33.0	40.0	24.0	41.0
15	35.30967830205651	36.0	33.0	40.0	29.0	41.0
16	35.707223728152464	35.0	34.0	40.0	31.0	41.0
17	35.681943249694996	35.0	34.0	40.0	30.0	41.0
18	35.61056467596631	36.0	34.0	40.0	30.0	41.0
19	35.45608344583716	36.0	34.0	40.0	29.0	41.0
20	35.30643622658396	35.0	34.0	40.0	29.0	41.0
21	35.09393779508632	35.0	34.0	40.0	29.0	41.0
22	34.9148722747744	35.0	33.0	40.0	28.0	41.0
23	35.010147265363386	35.0	34.0	39.0	29.0	41.0
24	34.86423572738446	35.0	34.0	39.0	29.0	41.0
25	34.730852554731285	35.0	34.0	39.0	29.0	41.0
26	34.447506950544174	35.0	33.0	39.0	27.0	41.0
27	34.43771042498477	35.0	33.0	39.0	27.0	41.0
28	34.59067908970926	35.0	33.0	39.0	27.0	41.0
29	34.71534290107172	35.0	34.0	39.0	29.0	41.0
30	34.71292173815762	35.0	34.0	39.0	29.0	41.0
31	34.49208265364898	35.0	33.0	39.0	27.0	41.0
32	34.279198710728856	35.0	33.0	39.0	27.0	41.0
33	34.118622620600824	35.0	33.0	39.0	25.0	41.0
34	34.00597164752464	35.0	33.0	39.0	25.0	41.0
35	33.849349695143616	35.0	33.0	39.0	24.0	41.0
36	33.58378070296118	35.0	33.0	39.0	23.0	41.0
37	33.45360634597156	35.0	32.0	39.0	23.0	41.0
38	33.49196397660324	35.0	32.0	39.0	23.0	41.0
39	33.57947280179119	35.0	33.0	39.0	23.0	41.0
40	33.29790584148073	35.0	32.0	39.0	23.0	41.0
41	33.3454847541571	35.0	32.0	39.0	23.0	41.0
42	33.285422830439984	35.0	32.0	39.0	23.0	41.0
43	33.19839400476825	35.0	32.0	39.0	23.0	41.0
44	33.04250603589943	35.0	32.0	38.0	23.0	41.0
45	32.9074273688921	35.0	32.0	38.0	23.0	41.0
46	32.88831960862276	35.0	32.0	38.0	23.0	40.0
47	32.861723855900365	35.0	32.0	38.0	23.0	40.0
48	32.83646454277589	35.0	32.0	38.0	23.0	40.0
49	32.79179132795835	35.0	32.0	38.0	22.0	40.0
50	32.586387818443775	35.0	32.0	38.0	21.0	40.0
51	32.198340335531	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	7.0
14	23.0
15	74.0
16	223.0
17	555.0
18	1196.0
19	2138.0
20	3643.0
21	5792.0
22	8359.0
23	11845.0
24	16460.0
25	22017.0
26	27668.0
27	32379.0
28	36565.0
29	42569.0
30	50629.0
31	60665.0
32	73980.0
33	96433.0
34	168632.0
35	204814.0
36	81765.0
37	94692.0
38	127230.0
39	152540.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.981285310200633	25.491149111282784	30.821562636535294	12.706002941981287
2	28.2892817241885	27.984500929006938	30.232259293470946	13.493958053333616
3	28.10937639369938	26.695456558909925	31.209341773261833	13.985825274128857
4	24.454425746720506	29.98281072598604	30.761846161288908	14.800917366004546
5	22.86596750516661	34.37809448507013	29.078824235515732	13.677113774247534
6	21.15059285609539	43.0397802433711	26.057094997573543	9.752531902959973
7	89.84736771288924	4.636266193369506	4.5119198620020295	1.004446231739231
8	91.15425143508517	3.470434297515039	3.729180493424385	1.6461337739754087
9	87.68752107084491	5.598003806736321	5.118760195265315	1.5957149271534594
10	56.805108101938295	26.556899218243306	8.29371132602323	8.344281353795171
11	54.75116371536255	18.39252319493725	16.244922209842183	10.61139087985801
12	49.368365990938216	20.260363832074248	18.614910372373796	11.75635980461374
13	21.42400360415385	48.48199208114184	18.339231910065475	11.754772404638835
14	13.720729478319896	50.15163449284083	23.567447113124167	12.56018891571511
15	11.821140841684821	23.523831409051805	52.55110294062065	12.103924808642713
16	14.234669117813803	17.988492106086696	51.428433205988576	16.34840557011092
17	15.113181618210653	18.700856742443598	25.024680290086003	41.161281349259745
18	21.166693627269414	23.329337116888574	35.49033273415284	20.013636521689175
19	30.391452833811318	24.88445995896949	23.57062191307398	21.153465294145217
20	34.17709941205729	23.675314720942644	23.530181008951423	18.617404858048648
21	21.57276565894485	30.069815362705775	28.242944763016304	20.114474215333075
22	23.271208041617093	25.03284406138551	23.836171251732914	27.859776645264482
23	18.258879235145336	32.31477687959496	22.181873706457996	27.24447017880171
24	20.336332259444653	24.023560039246576	39.843361417714476	15.796746283594295
25	15.52976072591045	25.02740154718584	38.22081187193764	21.222025854966066
26	15.831139949717215	38.00930972290043	25.45766253085981	20.701887796522538
27	16.182862429870937	39.50516963258494	27.65764771512671	16.65432022241741
28	12.405379622924475	32.42959881111301	39.92779597828437	15.237225587678147
29	12.905410615019223	25.12385499328001	39.31951942599617	22.651214965704604
30	16.1966954867951	35.11222917822571	30.96669634852651	17.724378986452674
31	30.69714071469282	28.60955856674412	23.56003924657462	17.133261471988437
32	31.681101927708294	27.551745459658118	25.52085616795599	15.246296444677599
33	28.89045277182713	30.31306551124106	22.387101846070582	18.409379870861233
34	18.985379290326385	31.12150564131715	25.4165413124623	24.47657375589417
35	18.24413909252123	27.92334823473564	30.936308977578353	22.89620369516478
36	34.46184873136506	22.958717017985997	25.875148724259557	16.70428552638939
37	19.644830594186487	33.847524941077225	30.11373342867812	16.393911036058167
38	18.971924185777198	35.62201134159487	21.60678137269279	23.799283099935142
39	18.98923440455115	33.882825692900084	27.627260344178552	19.50067955837021
40	25.584578938377135	26.505800057146402	22.87889347639083	25.030727528085638
41	16.16925614437176	23.571604589248917	28.15170705969682	32.1074322066825
42	22.535107996111627	24.66759088620761	23.940939650076572	28.85636146760419
43	22.258598038578356	26.0380461978747	25.61216946175046	26.091186301796483
44	18.201430474148815	30.95724753915209	29.174597367334936	21.666724619364164
45	16.133275078273936	41.30664183267595	21.059128381350924	21.500954707699194
46	23.89649245077926	33.79876908470518	24.02658365824639	18.27815480626917
47	21.535650735722093	28.88493466715246	25.321070542543072	24.25834405458237
48	24.29901173012991	24.43552812797165	30.520183412728528	20.745276729169912
49	22.11089425043729	21.669445876464	32.525069581032234	23.69459029206648
50	20.468842362111637	34.51650064478675	25.39189881761379	19.622758175487824
51	17.316643964327344	36.72608581937807	21.76839380823301	24.188876408061574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2105.0
1	2031.0
2	1957.0
3	4277.0
4	6597.0
5	5350.5
6	4104.0
7	4477.0
8	4850.0
9	5379.0
10	5908.0
11	6213.5
12	6519.0
13	6592.0
14	6665.0
15	6708.0
16	6751.0
17	6263.5
18	5776.0
19	5904.5
20	6033.0
21	5856.0
22	5679.0
23	5977.5
24	6276.0
25	6637.0
26	8170.0
27	9342.0
28	10302.5
29	11263.0
30	14568.0
31	17873.0
32	20259.0
33	22645.0
34	26148.5
35	29652.0
36	30912.5
37	32173.0
38	39855.5
39	47538.0
40	77619.5
41	107701.0
42	131494.5
43	155288.0
44	157890.0
45	160492.0
46	147809.5
47	135127.0
48	120997.5
49	106868.0
50	98652.0
51	90436.0
52	84074.5
53	77713.0
54	66461.5
55	55210.0
56	51909.5
57	48609.0
58	43395.0
59	38181.0
60	36102.0
61	34023.0
62	30377.5
63	26732.0
64	21286.5
65	15841.0
66	12438.5
67	9036.0
68	7354.0
69	5672.0
70	4629.0
71	3586.0
72	2963.0
73	2340.0
74	1739.5
75	1213.5
76	1288.0
77	905.0
78	522.0
79	373.5
80	225.0
81	155.0
82	85.0
83	62.5
84	40.0
85	35.5
86	31.0
87	23.5
88	16.0
89	11.0
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1322918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.298070984158333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41902158563512	14.256900858331942
2	7.192066212173734	2.48817743721896
3	2.1969912824943254	1.1401113346849168
4	0.9661930553064838	0.6685310422036951
5	0.5551818751495406	0.4801787742727442
6	0.35499333546546424	0.3684419949470839
7	0.2616614075767077	0.3168366321453672
8	0.2029611221566881	0.2808668718472657
9	0.1521872372737674	0.23692910699201183
>10	2.0888810866173566	9.53232666524105
>50	1.8149669671115163	23.51308417483869
>100	1.7873120268360667	45.230325210106805
>500	0.005798616508327644	0.5553804046392296
>1k	0.0013381422711525332	0.49404011265698616
>5k	4.460474237175111E-4	0.4378693798732463
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5675	0.428975945599047	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3602	0.27227689093352725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1695	0.1281258551172484	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5118094999085356E-4	0.0	0.0	0.026381075773403945	0.0
2	1.5118094999085356E-4	0.0	0.0	0.1278234932172667	0.0
3	2.2677142498628034E-4	0.0	0.0	0.18806910178862182	0.0
4	2.2677142498628034E-4	0.0	0.0	0.3622295561780851	0.0
5	2.2677142498628034E-4	0.0	0.0	0.5893789335393426	0.0
6	2.2677142498628034E-4	0.0	0.0	0.8784369099218545	0.0
7	2.2677142498628034E-4	0.0	0.0	0.995753327114757	0.0
8	2.2677142498628034E-4	0.0	0.0	1.2788396559726303	0.0
9	2.2677142498628034E-4	0.0	0.0	1.3552616261930066	0.0
10	2.2677142498628034E-4	0.0	0.0	1.5107512332585995	0.0
11	2.2677142498628034E-4	0.0	0.0	1.7927037049915415	0.0
12	2.2677142498628034E-4	0.0	0.0	1.9877271304797426	0.0
13	2.2677142498628034E-4	0.0	0.0	2.0690624815748215	0.0
14	2.2677142498628034E-4	0.0	0.0	2.1063285857475673	0.0
15	2.2677142498628034E-4	0.0	0.0	2.1697489942687302	0.0
16	2.2677142498628034E-4	0.0	0.0	2.3120102682101233	0.0
17	2.2677142498628034E-4	0.0	0.0	2.4650053896008672	0.0
18	2.2677142498628034E-4	0.0	0.0	2.681042967137797	0.0
19	2.2677142498628034E-4	0.0	0.0	2.7756066513570756	0.0
20	2.2677142498628034E-4	0.0	0.0	2.87024592605135	0.0
21	2.2677142498628034E-4	0.0	0.0	3.0040410667932553	0.0
22	2.2677142498628034E-4	0.0	0.0	3.142825178884859	0.0
23	2.2677142498628034E-4	0.0	0.0	3.3152470523494277	0.0
24	2.2677142498628034E-4	0.0	0.0	3.4236437934928694	0.0
25	2.2677142498628034E-4	0.0	0.0	3.5143523634873817	0.0
26	2.2677142498628034E-4	0.0	0.0	3.6216152475058925	0.0
27	2.2677142498628034E-4	0.0	0.0	3.706578941400752	0.0
28	2.2677142498628034E-4	0.0	0.0	3.8018985303699853	0.0
29	2.2677142498628034E-4	0.0	0.0	3.8963866241142684	0.0
30	2.2677142498628034E-4	0.0	0.0	4.044770726530292	0.0
31	2.2677142498628034E-4	0.0	0.0	4.178112324422224	0.0
32	2.2677142498628034E-4	0.0	0.0	4.296184646365081	0.0
33	2.2677142498628034E-4	0.0	0.0	4.407000282708377	0.0
34	2.2677142498628034E-4	0.0	0.0	4.530666299800894	0.0
35	2.2677142498628034E-4	0.0	0.0	4.722212563439306	0.0
36	2.2677142498628034E-4	0.0	0.0	4.859409275556006	0.0
37	2.2677142498628034E-4	0.0	0.0	5.006508339897106	0.0
38	2.2677142498628034E-4	0.0	0.0	5.129645223664657	0.0
39	2.2677142498628034E-4	0.0	0.0	5.2584513930568635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.0336723E-4	45.000004	44
CTAGACG	20	7.0336723E-4	45.000004	36
TCGTTGC	20	7.0336723E-4	45.000004	38
CACGGTC	45	3.8562575E-10	45.000004	28
GTCGAAG	80	0.0	45.000004	1
GCGAATC	20	7.0336723E-4	45.000004	35
CGTGATA	40	6.8157533E-9	45.000004	39
CGTTGAT	90	0.0	45.000004	25
TAGCGGC	20	7.0336723E-4	45.000004	44
TCGATAG	40	6.8157533E-9	45.000004	1
ATGTACG	45	3.8562575E-10	45.000004	1
CCCGTAG	20	7.0336723E-4	45.000004	29
TACGCGG	90	0.0	45.000004	2
TCTACCG	20	7.0336723E-4	45.000004	41
CTACGGA	45	3.8562575E-10	45.000004	11
TTGCGCA	20	7.0336723E-4	45.000004	2
ACTAAGA	20	7.0336723E-4	45.000004	30
CAAGATT	40	6.8157533E-9	45.000004	26
ACTCCGA	20	7.0336723E-4	45.000004	39
TGTCGAA	20	7.0336723E-4	45.000004	12
>>END_MODULE