FastQCFastQC Report
Thu 26 May 2016
SRR1548023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548023_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1552533
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.2777396680134979No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21750.14009364052165074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA351.2123019E-745.00000439
CGTAACG253.8914397E-545.027
TGTCGAT253.8914397E-545.031
TCGTTAG556.184564E-1140.9090921
TACGGGA5450.040.4587144
CGTAAGG1900.040.2631572
ACGGGAT7850.040.1273885
CTACGTT451.9290383E-840.030
TCCGTAC451.9290383E-840.040
CTCACGA1750.039.85714324
TACGCTA403.459263E-739.37544
CGTTAGG2250.039.02
CGACGGT1850.038.9189228
CGTTTTT26000.038.769231
CGACAAC356.249704E-638.5714327
TCGATAG700.038.571431
TATCGGA356.249704E-638.5714338
CGGGTAT1300.038.0769276
ATTGGGA14850.038.03034
AACGGGA6700.037.947764