##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548023_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1552533
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.930353815345633	31.0	31.0	33.0	30.0	33.0
2	31.2731516818	31.0	31.0	33.0	30.0	34.0
3	31.488228591598375	31.0	31.0	34.0	30.0	34.0
4	35.36708012003609	37.0	35.0	37.0	33.0	37.0
5	35.195680864754564	37.0	35.0	37.0	32.0	37.0
6	35.23023729608324	37.0	35.0	37.0	32.0	37.0
7	35.52232319699485	35.0	35.0	37.0	35.0	37.0
8	35.601243902706095	37.0	35.0	37.0	35.0	37.0
9	37.45444895535232	39.0	37.0	39.0	35.0	39.0
10	36.63303775185455	39.0	37.0	39.0	32.0	39.0
11	36.269960123230874	38.0	35.0	39.0	32.0	39.0
12	35.58511864160053	37.0	35.0	39.0	30.0	39.0
13	35.22271925942959	37.0	35.0	39.0	30.0	39.0
14	36.20957300102478	38.0	35.0	40.0	30.0	41.0
15	36.49007203067503	38.0	35.0	40.0	30.0	41.0
16	36.66297914440466	38.0	35.0	40.0	31.0	41.0
17	36.591041221023964	38.0	35.0	40.0	31.0	41.0
18	36.52422267352771	38.0	35.0	40.0	31.0	41.0
19	36.45313497362053	38.0	35.0	40.0	31.0	41.0
20	36.32085759207695	38.0	35.0	40.0	30.0	41.0
21	36.17020443365777	38.0	34.0	40.0	30.0	41.0
22	36.04560418361478	38.0	34.0	40.0	30.0	41.0
23	36.010371438159446	38.0	34.0	40.0	30.0	41.0
24	35.89974899084271	38.0	34.0	40.0	30.0	41.0
25	35.73897495254529	37.0	34.0	40.0	29.0	41.0
26	35.485009980464184	37.0	34.0	40.0	29.0	41.0
27	35.5661051971198	37.0	34.0	40.0	29.0	41.0
28	35.614517694631935	37.0	34.0	40.0	29.0	41.0
29	35.64221694482501	37.0	34.0	40.0	29.0	41.0
30	35.59933540865154	37.0	34.0	40.0	29.0	41.0
31	35.51287798713457	37.0	34.0	40.0	29.0	41.0
32	35.38653735540565	37.0	34.0	40.0	28.0	41.0
33	35.26237831981671	37.0	34.0	40.0	27.0	41.0
34	35.14769798774004	37.0	34.0	40.0	27.0	41.0
35	35.00043348514975	37.0	34.0	40.0	26.0	41.0
36	34.89432623976431	37.0	33.0	40.0	25.0	41.0
37	34.83070247138064	37.0	33.0	40.0	25.0	41.0
38	34.7664848347829	37.0	33.0	40.0	25.0	41.0
39	34.751681284713435	37.0	33.0	40.0	25.0	41.0
40	34.54382612157036	37.0	33.0	40.0	24.0	41.0
41	34.47119256080225	37.0	33.0	40.0	24.0	41.0
42	34.42965399125172	36.0	33.0	40.0	24.0	41.0
43	34.401136078911044	36.0	33.0	40.0	24.0	41.0
44	34.205849408676016	36.0	33.0	40.0	23.0	41.0
45	34.074972963537654	36.0	33.0	40.0	23.0	41.0
46	34.0449781099661	36.0	33.0	40.0	23.0	41.0
47	34.00147565301349	36.0	33.0	40.0	23.0	41.0
48	33.941565815348206	35.0	33.0	40.0	23.0	41.0
49	33.81103654479486	35.0	33.0	40.0	24.0	41.0
50	33.70032521047862	35.0	32.0	39.0	23.0	41.0
51	33.346502135542366	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	12.0
14	26.0
15	81.0
16	216.0
17	466.0
18	917.0
19	1926.0
20	3077.0
21	4681.0
22	6966.0
23	10049.0
24	14493.0
25	19279.0
26	25104.0
27	29587.0
28	35060.0
29	41422.0
30	49918.0
31	60837.0
32	74754.0
33	95578.0
34	147427.0
35	171513.0
36	127765.0
37	157197.0
38	215065.0
39	259084.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.79805775464998	24.316584575013863	28.495690590795814	13.389667079540338
2	28.492083582120316	27.46357082264918	29.60130316070576	14.443042434524742
3	28.9764533185446	26.98248604055437	30.25153088533384	13.789529755567193
4	25.64737754366574	29.185530999985186	30.250371489688142	14.916719966660935
5	22.892524667752635	34.176536022100656	29.11017028301492	13.82076902713179
6	21.883657223389132	42.18132561433477	25.88279927061132	10.052217891664784
7	91.73035291359346	3.107244741335611	4.168607044101478	0.993795300969448
8	92.23572059337869	2.4808490383135173	3.955729121377774	1.327701246930017
9	87.78892300517927	5.178118597157033	4.956545207090606	2.0764131905730827
10	43.60725343680296	34.11714920069332	11.380370014679238	10.895227347824491
11	38.66004780574712	23.753504756420636	21.543889888330874	16.042557549501364
12	36.252176282243276	22.1405921806493	24.383121002902996	17.224110534204424
13	24.64804290794463	31.725573627098424	24.8328376916948	18.793545773262146
14	18.60913745472721	34.57214758075996	27.564309422086357	19.254405542426472
15	18.212817376506653	25.99854560257334	37.937615496739845	17.85102152418016
16	21.762822432759883	23.40124171273654	35.72239688302922	19.11353897147436
17	21.215652098860378	24.09063124584147	26.413544832863455	28.280171822434692
18	23.911375796842965	25.5627416615299	29.973211519497493	20.552671022129644
19	28.129063923278924	26.40143558945285	24.77441703332554	20.695083453942686
20	29.947640404422966	25.796681938483758	25.468250916405644	18.78742674068764
21	26.21747814700235	27.028282168559382	27.51271631585287	19.241523368585405
22	26.104243838939333	22.997385562818952	27.23343078697844	23.66493981126327
23	22.65748940602229	27.697253456126212	26.402594985098542	23.242662152752953
24	22.38058707930846	25.30194205211741	32.66571467401981	19.651756194554316
25	21.709683465665464	26.671059487946476	29.940555208810377	21.678701837577687
26	21.114462623338763	31.86618255457372	25.459555449062922	21.5597993730246
27	20.000218996955297	31.267934401394363	28.187291349040567	20.54455525260977
28	18.42756321443731	29.22978126712927	32.734312249723516	19.60834326870991
29	19.191669355820455	26.589644149270903	32.26044148497971	21.958245009928937
30	20.732699401558612	28.754686695870557	30.017461786641576	20.49515211592926
31	26.497729838914857	27.146669346158824	25.845505377341414	20.5100954375849
32	27.33346086685436	27.578801867657564	25.838549003467236	19.249188262020837
33	25.798614264559916	27.176105113385674	26.3034666573915	20.721813964662907
34	20.945319680805497	27.236586919569504	28.674237520233063	23.143855879391936
35	21.581054959862367	27.44836985751672	29.02881935520855	21.941755827412365
36	27.425117533733584	26.728771626754472	26.236286120810316	19.60982471870163
37	21.989291048885917	29.74410205773404	27.64984705639107	20.616759836988972
38	21.51606439283416	30.603214231195086	25.848532688194066	22.032188687776685
39	22.07399134189096	29.059156874604277	26.940554564701685	21.92629721880308
40	24.895960343516048	25.705540558558177	26.20820298183678	23.190296116088998
41	20.088333066028227	25.443839196976814	28.436303769388477	26.031523967606486
42	22.929303274068893	25.92022198561963	26.050267530545245	25.100207209766236
43	22.516171958985733	26.314287683418	26.882520371547656	24.287019986048605
44	21.382025374017815	28.709856730903628	27.95296460687148	21.95515328820708
45	20.38236868395068	31.924925267289005	24.741503079161603	22.951202969598715
46	22.376078318464085	29.91691641981201	26.29155064658851	21.415454615135395
47	22.173506134813238	27.88629935724394	27.509431361523397	22.430763146419434
48	23.215029889863857	26.141924197424466	29.252582714827962	21.390463197883715
49	22.273665036427566	25.30155558690218	29.636664727899504	22.78811464877075
50	21.26267203338029	29.53599053933153	27.39143064913918	21.809906778149
51	20.325365064703938	30.271691487395113	26.120990664932727	23.281952782968222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1264.0
1	1709.5
2	2155.0
3	5249.5
4	8344.0
5	6571.0
6	4798.0
7	4628.0
8	4458.0
9	4622.5
10	4787.0
11	5007.0
12	5227.0
13	5056.0
14	4885.0
15	4687.0
16	4489.0
17	4669.5
18	4850.0
19	5007.5
20	5165.0
21	6165.5
22	7166.0
23	7331.5
24	7497.0
25	8300.0
26	12175.5
27	15248.0
28	18036.0
29	20824.0
30	25185.5
31	29547.0
32	34425.0
33	39303.0
34	42492.5
35	45682.0
36	49556.0
37	53430.0
38	59736.5
39	66043.0
40	80113.0
41	94183.0
42	105481.5
43	116780.0
44	120342.0
45	123904.0
46	121822.0
47	119740.0
48	118344.0
49	116948.0
50	110535.0
51	104122.0
52	100264.0
53	96406.0
54	90651.5
55	84897.0
56	80768.5
57	76640.0
58	72105.5
59	67571.0
60	63020.0
61	58469.0
62	52422.0
63	46375.0
64	40578.0
65	34781.0
66	29721.0
67	24661.0
68	20258.5
69	15856.0
70	13533.5
71	11211.0
72	8684.5
73	6158.0
74	5296.0
75	3554.5
76	2675.0
77	1898.0
78	1121.0
79	977.0
80	833.0
81	532.5
82	232.0
83	177.0
84	122.0
85	104.0
86	86.0
87	61.5
88	37.0
89	26.0
90	15.0
91	11.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1552533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.982874583865915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27778958358783	15.049203369513595
2	5.717820094721953	2.170813235024303
3	1.851606579577737	1.0544644643635537
4	1.002346033126931	0.7610963614593615
5	0.6822262815315354	0.6475307970065167
6	0.500599492083678	0.5701690424982863
7	0.4148915444200476	0.5513083907542538
8	0.3384522013819915	0.513983655317414
9	0.31396968542772047	0.5364042445449226
>10	6.857783372681581	35.51386851349015
>50	2.802680376026893	35.873140467509366
>100	0.2367245064424724	5.999072207512837
>500	0.002073499326502899	0.2550591352674826
>1k	0.0010367496632514494	0.5038861157380488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4312	0.2777396680134979	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2175	0.14009364052165074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018421508592732005	0.0
2	0.0	0.0	0.0	0.07175370829476732	0.0
3	0.0	0.0	0.0	0.10479648419711529	0.0
4	0.0	0.0	0.0	0.18569653591904325	0.0
5	0.0	0.0	0.0	0.3153556156294262	0.0
6	0.0	0.0	0.0	0.4723893147520858	0.0
7	0.0	0.0	0.0	0.5384748665567817	0.0
8	0.0	0.0	0.0	0.6846875396529414	0.0
9	0.0	0.0	0.0	0.7275851785437089	0.0
10	0.0	0.0	0.0	0.8239438388749225	0.0
11	0.0	0.0	0.0	0.9923138509777248	0.0
12	0.0	0.0	0.0	1.1184947437510184	0.0
13	0.0	0.0	0.0	1.1773018673355091	0.0
14	0.0	0.0	0.0	1.2069308671699732	0.0
15	0.0	0.0	0.0	1.2559475386352497	0.0
16	0.0	0.0	0.0	1.3635136902081952	0.0
17	0.0	0.0	0.0	1.479066789562605	0.0
18	0.0	0.0	0.0	1.6129769866405417	0.0
19	0.0	0.0	0.0	1.6911073709866393	0.0
20	0.0	0.0	0.0	1.7774179357218172	0.0
21	0.0	0.0	0.0	1.891167530738477	0.0
22	0.0	0.0	0.0	2.005883288793217	0.0
23	0.0	0.0	0.0	2.1318065380896893	0.0
24	0.0	0.0	0.0	2.2295178266742157	0.0
25	0.0	0.0	0.0	2.3128010805567416	0.0
26	0.0	0.0	0.0	2.396857264869732	0.0
27	0.0	0.0	0.0	2.4801405187522585	0.0
28	0.0	0.0	0.0	2.5697360378169094	0.0
29	0.0	0.0	0.0	2.666287930755739	0.0
30	0.0	0.0	0.0	2.7860921474777025	0.0
31	0.0	0.0	0.0	2.8980382381566123	0.0
32	0.0	0.0	0.0	2.9991633028090225	0.0
33	0.0	0.0	0.0	3.1060853456899147	0.0
34	0.0	0.0	0.0	3.224665755896976	0.0
35	0.0	0.0	0.0	3.36263383773485	0.0
36	0.0	0.0	0.0	3.473678176244885	0.0
37	0.0	0.0	0.0	3.6021778603095713	0.0
38	0.0	0.0	0.0	3.7344777856573743	0.0
39	0.0	0.0	0.0	3.9139264672634977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	35	1.2123019E-7	45.000004	39
CGTAACG	25	3.8914397E-5	45.0	27
TGTCGAT	25	3.8914397E-5	45.0	31
TCGTTAG	55	6.184564E-11	40.909092	1
TACGGGA	545	0.0	40.458714	4
CGTAAGG	190	0.0	40.263157	2
ACGGGAT	785	0.0	40.127388	5
CTACGTT	45	1.9290383E-8	40.0	30
TCCGTAC	45	1.9290383E-8	40.0	40
CTCACGA	175	0.0	39.857143	24
TACGCTA	40	3.459263E-7	39.375	44
CGTTAGG	225	0.0	39.0	2
CGACGGT	185	0.0	38.91892	28
CGTTTTT	2600	0.0	38.76923	1
CGACAAC	35	6.249704E-6	38.57143	27
TCGATAG	70	0.0	38.57143	1
TATCGGA	35	6.249704E-6	38.57143	38
CGGGTAT	130	0.0	38.076927	6
ATTGGGA	1485	0.0	38.0303	4
AACGGGA	670	0.0	37.94776	4
>>END_MODULE