FastQCFastQC Report
Thu 26 May 2016
SRR1548022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548022_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2313852
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178150.7699282408727957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98050.042.085671
GCGTAAG1700.041.029411
TGCGCGG2550.039.7058832
TAATGCG850.039.7058831
GACCGAT15050.039.617949
CGTAAGG3900.038.6538472
CGGTCTA1050.038.57142631
TACGGGA12850.038.5214044
AGGGCGA19000.038.486846
GGGCGAT36450.038.395067
TAAGGGA42200.038.388634
CGACCCG1700.038.3823532
GGACCGA16350.038.2568788
CGGGTAT1300.038.076926
GGGACCG19450.038.0591247
TTAGGGA41000.038.030494
ATAGGGA42900.037.8671344
CGAACGG1250.037.8000032
TAGGGCG7500.037.85
TAGGGAT48450.037.755425
Produced by FastQC (version 0.11.5)