##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548022_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2313852
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.911079878920518	31.0	31.0	33.0	30.0	33.0
2	31.241980472389763	31.0	31.0	33.0	30.0	34.0
3	31.321123390778666	31.0	31.0	34.0	30.0	34.0
4	35.251975925858694	37.0	35.0	37.0	33.0	37.0
5	35.12976067613659	37.0	35.0	37.0	32.0	37.0
6	35.17151961318183	37.0	35.0	37.0	32.0	37.0
7	35.585483427634955	36.0	35.0	37.0	35.0	37.0
8	35.69276816321874	37.0	35.0	37.0	35.0	37.0
9	37.53082349260022	39.0	37.0	39.0	35.0	39.0
10	36.69127325343194	39.0	37.0	39.0	32.0	39.0
11	36.24706679597485	38.0	35.0	39.0	31.0	39.0
12	35.96971932517724	37.0	35.0	39.0	31.0	39.0
13	35.85167201705208	38.0	35.0	39.0	30.0	39.0
14	36.89669434345844	39.0	35.0	41.0	31.0	41.0
15	37.055290485303296	39.0	35.0	41.0	31.0	41.0
16	37.11454405899772	39.0	35.0	41.0	31.0	41.0
17	37.056082238622004	39.0	35.0	41.0	31.0	41.0
18	36.95842171409407	39.0	35.0	40.0	31.0	41.0
19	36.89715418272215	38.0	35.0	40.0	31.0	41.0
20	36.74815156717024	38.0	35.0	40.0	31.0	41.0
21	36.6309266971267	38.0	35.0	40.0	31.0	41.0
22	36.5237361767304	38.0	35.0	40.0	30.0	41.0
23	36.4490356340855	38.0	35.0	40.0	30.0	41.0
24	36.3576957385347	38.0	35.0	40.0	30.0	41.0
25	36.197255485657685	38.0	35.0	40.0	30.0	41.0
26	35.97851893725269	38.0	34.0	40.0	30.0	41.0
27	36.04824681958915	38.0	34.0	40.0	30.0	41.0
28	36.07011079360305	38.0	35.0	40.0	30.0	41.0
29	36.065905252367045	38.0	35.0	40.0	30.0	41.0
30	35.98398990082339	38.0	35.0	40.0	30.0	41.0
31	35.89657462966517	38.0	34.0	40.0	29.0	41.0
32	35.747561209619285	38.0	34.0	40.0	29.0	41.0
33	35.58150650949153	38.0	34.0	40.0	28.0	41.0
34	35.38973235971877	38.0	34.0	40.0	27.0	41.0
35	35.19379675104544	38.0	34.0	40.0	25.0	41.0
36	35.110631535638404	38.0	34.0	40.0	25.0	41.0
37	35.06816252724894	38.0	34.0	40.0	25.0	41.0
38	35.00396654582921	38.0	34.0	40.0	25.0	41.0
39	34.97212094809867	38.0	34.0	40.0	25.0	41.0
40	34.822662815080655	38.0	34.0	40.0	24.0	41.0
41	34.742398822396595	38.0	33.0	40.0	24.0	41.0
42	34.71427083495401	38.0	33.0	40.0	24.0	41.0
43	34.68659577189898	38.0	33.0	40.0	24.0	41.0
44	34.56221962338127	38.0	33.0	40.0	23.0	41.0
45	34.4615105028325	37.0	33.0	40.0	23.0	41.0
46	34.41593498633448	37.0	33.0	40.0	23.0	41.0
47	34.37777653886247	37.0	33.0	40.0	23.0	41.0
48	34.28908374433628	37.0	33.0	40.0	23.0	41.0
49	34.16254842574201	37.0	33.0	40.0	23.0	41.0
50	34.06429235750601	37.0	33.0	40.0	23.0	41.0
51	33.759019159393084	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	15.0
15	68.0
16	200.0
17	449.0
18	1145.0
19	2154.0
20	3646.0
21	5881.0
22	9247.0
23	13767.0
24	20504.0
25	30216.0
26	39767.0
27	45546.0
28	50227.0
29	57217.0
30	67566.0
31	82391.0
32	100941.0
33	126880.0
34	178081.0
35	211804.0
36	205213.0
37	253007.0
38	352276.0
39	455591.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.348656698872702	24.940229539313663	29.88786663969865	14.823247122114985
2	31.124505802445444	26.41733352003499	29.80359158667019	12.654569090849371
3	27.773729694034017	26.21157273671782	32.96485687070738	13.049840698540788
4	24.146877155496547	28.686190819464684	33.021256329272575	14.145675695766194
5	22.423949327787604	33.11253269439878	30.840736572607064	13.622781405206554
6	21.307369702124422	41.4598254339517	27.389824414007464	9.842980449916416
7	90.4182722144718	2.3447912831071305	6.29050604792355	0.946430454497522
8	91.40753168309814	1.7376651574949478	5.918139967465508	0.9366631919414034
9	86.52895690822058	5.1507183691956095	6.837818494873484	1.482506227710329
10	37.93838153866367	42.39951388420694	11.643009146652423	8.01909543047697
11	25.422282842636434	26.571448822137285	31.09788352928364	16.908384805942646
12	27.06283720825705	24.544050354128093	30.531122993173287	17.861989444441566
13	23.045121295571196	27.919979324520323	31.08500457246185	17.949894807446633
14	18.088451638220597	31.571682199207206	31.063179494626276	19.27668666794592
15	17.468230465907066	29.142270119264325	36.420825532488685	16.968673882339925
16	21.82391095022499	27.691226578017954	34.465601084252576	16.019261387504475
17	21.049920219616467	27.31695890661978	30.36006624451348	21.27305462925027
18	21.231867898206108	28.091252163059693	32.203745096920635	18.473134841813565
19	23.805541581743345	28.99960758077872	28.9348670528625	18.25998378461544
20	25.413034195791262	29.394014828951896	28.845967676411455	16.346983298845387
21	24.21166090138868	27.35252730079538	31.473231650079608	16.96258014773633
22	23.09629137905104	25.377725109471132	31.250961599964043	20.27502191151379
23	20.14199698165656	28.76104435374432	31.580369012365527	19.51658965223359
24	19.822097523955723	28.3143865726935	33.96855114328833	17.89496476006244
25	20.21058390942895	30.242426914080934	30.9130834642838	18.63390571220631
26	19.63600091967853	32.758923215486554	28.865804727355076	18.739271137479836
27	18.580920473738168	31.865305127553533	31.47037926366941	18.083395135038888
28	17.993717835021428	30.560035819058434	33.296079438097166	18.150166907822975
29	18.54059810221224	28.772064937601886	33.36872885560528	19.318608104580587
30	20.098865441696358	29.626613975310434	31.696366059713416	18.578154523279792
31	22.076952199189922	29.35304418778729	29.599256996558122	18.970746616464666
32	22.459171978155908	29.685865820285827	29.7770125314843	18.077949670073973
33	22.098345097266375	29.79205238710168	29.590656619351623	18.518945896280314
34	20.227741445866027	29.819798327637205	30.18365046684058	19.768809759656193
35	19.758783189244603	29.952693603566694	30.499357780877943	19.78916542631076
36	22.39451788619151	29.76694274309679	29.162755439846627	18.675783930865066
37	20.054264490555145	31.414109459031952	30.146569443508053	18.385056606904847
38	19.9204616371315	31.375991204277543	28.99813816959771	19.705408988993245
39	19.943237510437143	31.085134226389588	29.254852946515165	19.716775316658108
40	21.81833583133234	29.61481546788645	29.107177122823757	19.45967157795745
41	18.954280567642183	29.41752540784804	30.317150794432834	21.311043230076944
42	20.50023078399137	30.187237558841275	28.758667365069158	20.553864292098197
43	20.660915218432294	29.800912072163648	29.012529755576416	20.525642953827646
44	20.00685437097965	30.880583546397954	29.10592380152231	20.006638281100088
45	19.560023718025178	31.525352529029515	28.13693356359871	20.777690189346597
46	20.385746365800404	31.36782300683017	28.345762823205632	19.900667804163792
47	20.014287862836515	30.59443732788441	29.42271156495748	19.96856324432159
48	20.429396521471553	29.45737238163893	30.14950826587007	19.963722831019442
49	20.331032408295776	28.947400265876986	30.175741577248676	20.54582574857856
50	19.465808530536957	31.309738047204398	29.31505558696062	19.909397835298023
51	18.89541768445	31.401057630306518	29.06473707047815	20.638787614765334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1521.0
1	1794.0
2	2067.0
3	15549.5
4	29032.0
5	21766.0
6	14500.0
7	13827.0
8	13154.0
9	14431.5
10	15709.0
11	16596.5
12	17484.0
13	17717.5
14	17951.0
15	17292.5
16	16634.0
17	15868.0
18	15102.0
19	15184.0
20	15266.0
21	16080.5
22	16895.0
23	18662.5
24	20430.0
25	22921.0
26	30949.0
27	36486.0
28	42892.5
29	49299.0
30	56398.5
31	63498.0
32	70961.5
33	78425.0
34	88847.5
35	99270.0
36	105601.5
37	111933.0
38	121571.0
39	131209.0
40	140703.0
41	150197.0
42	162148.0
43	174099.0
44	176786.5
45	179474.0
46	176681.5
47	173889.0
48	169782.0
49	165675.0
50	156345.5
51	147016.0
52	137291.5
53	127567.0
54	115176.5
55	102786.0
56	94096.5
57	85407.0
58	78844.5
59	72282.0
60	63093.5
61	53905.0
62	46299.5
63	38694.0
64	30534.0
65	22374.0
66	17614.0
67	12854.0
68	9940.5
69	7027.0
70	5559.5
71	4092.0
72	3231.5
73	2371.0
74	1866.5
75	1122.0
76	882.0
77	609.0
78	336.0
79	225.5
80	115.0
81	93.5
82	72.0
83	55.0
84	38.0
85	32.5
86	27.0
87	26.0
88	25.0
89	15.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2313852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.64935995567252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18895708846573	16.576830338769298
2	7.266421791863633	3.291596055073246
3	3.257796049947258	2.213609861922706
4	1.8812108037755804	1.7043288258885254
5	1.3426671820208225	1.520527615312904
6	0.9773368749283812	1.3281632806922996
7	0.7923949633260713	1.256306712600388
8	0.692759679480085	1.2552450674656612
9	0.6040000633671686	1.231219336360681
>10	8.54549936587211	45.37664624502683
>50	1.3536012059729434	20.182592084320948
>100	0.09539218738894704	2.908396729628949
>500	9.813717956783596E-4	0.1391437934971558
>1k	7.850974365426875E-4	0.2234302575452732
>5k	0.0	0.0
>10k+	1.9627435913567188E-4	0.7919637958951536
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17815	0.7699282408727957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.321797591202895E-5	0.0	0.0	0.022862309257463312	0.0
2	4.321797591202895E-5	0.0	0.0	0.06149917972281719	0.0
3	4.321797591202895E-5	0.0	0.0	0.10575438705673483	0.0
4	4.321797591202895E-5	0.0	0.0	0.15791848398255376	0.0
5	4.321797591202895E-5	0.0	0.0	0.2773729694034018	0.0
6	4.321797591202895E-5	0.0	0.0	0.49934049368758243	0.0
7	4.321797591202895E-5	0.0	0.0	0.631544282002479	0.0
8	4.321797591202895E-5	0.0	0.0	0.8643163002646669	0.0
9	4.321797591202895E-5	0.0	0.0	0.9966497425072995	0.0
10	4.321797591202895E-5	0.0	0.0	1.1534877770920526	0.0
11	4.321797591202895E-5	0.0	0.0	1.3545810190107233	0.0
12	4.321797591202895E-5	0.0	0.0	1.5086963211130184	0.0
13	4.321797591202895E-5	0.0	0.0	1.602176803010737	0.0
14	4.321797591202895E-5	0.0	0.0	1.6714552183977194	0.0
15	4.321797591202895E-5	0.0	0.0	1.7308799352767592	0.0
16	4.321797591202895E-5	0.0	0.0	1.8240146733671816	0.0
17	4.321797591202895E-5	0.0	0.0	1.9398820667873313	0.0
18	4.321797591202895E-5	0.0	0.0	2.0616703229074287	0.0
19	4.321797591202895E-5	0.0	0.0	2.1580896271671652	0.0
20	4.321797591202895E-5	0.0	0.0	2.2535581359568373	0.0
21	4.321797591202895E-5	0.0	0.0	2.3677400283164176	0.0
22	4.321797591202895E-5	0.0	0.0	2.511396580248002	0.0
23	4.321797591202895E-5	0.0	0.0	2.6563928894328592	0.0
24	4.321797591202895E-5	0.0	0.0	2.778008273649309	0.0
25	4.321797591202895E-5	0.0	0.0	2.8786629395484242	0.0
26	4.321797591202895E-5	0.0	0.0	2.983250441255534	0.0
27	4.321797591202895E-5	0.0	0.0	3.09626544826549	0.0
28	4.321797591202895E-5	0.0	0.0	3.208329659805381	0.0
29	4.321797591202895E-5	0.0	0.0	3.343126526674999	0.0
30	4.321797591202895E-5	0.0	0.0	3.49693930294591	0.0
31	4.321797591202895E-5	0.0	0.0	3.635323261816227	0.0
32	4.321797591202895E-5	0.0	0.0	3.777510402566802	0.0
33	4.321797591202895E-5	0.0	0.0	3.9097141908816986	0.0
34	4.321797591202895E-5	0.0	0.0	4.055747731488444	0.0
35	4.321797591202895E-5	0.0	0.0	4.219284552339562	0.0
36	4.321797591202895E-5	0.0	0.0	4.379191063214069	0.0
37	4.321797591202895E-5	0.0	0.0	4.548432656885574	0.0
38	4.321797591202895E-5	0.0	0.0	4.722298573979667	0.0
39	4.321797591202895E-5	0.0	0.0	4.969332524292824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9805	0.0	42.08567	1
GCGTAAG	170	0.0	41.02941	1
TGCGCGG	255	0.0	39.705883	2
TAATGCG	85	0.0	39.705883	1
GACCGAT	1505	0.0	39.61794	9
CGTAAGG	390	0.0	38.653847	2
CGGTCTA	105	0.0	38.571426	31
TACGGGA	1285	0.0	38.521404	4
AGGGCGA	1900	0.0	38.48684	6
GGGCGAT	3645	0.0	38.39506	7
TAAGGGA	4220	0.0	38.38863	4
CGACCCG	170	0.0	38.38235	32
GGACCGA	1635	0.0	38.256878	8
CGGGTAT	130	0.0	38.07692	6
GGGACCG	1945	0.0	38.059124	7
TTAGGGA	4100	0.0	38.03049	4
ATAGGGA	4290	0.0	37.867134	4
CGAACGG	125	0.0	37.800003	2
TAGGGCG	750	0.0	37.8	5
TAGGGAT	4845	0.0	37.75542	5
>>END_MODULE