FastQCFastQC Report
Thu 26 May 2016
SRR1548021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548021_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1110796
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.31607964018595675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32360.2913226190947753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC700.045.0000046
CAAGTAG850.045.0000041
TAGTTCG351.2118835E-745.0000041
CCACTAG351.2118835E-745.00000420
CTATGCG207.0331857E-445.01
GTACCTC207.0331857E-445.033
GTACCGA207.0331857E-445.027
CGTATCA207.0331857E-445.022
CGTTGGC207.0331857E-445.028
GACCGAA253.8907212E-545.09
CGTTACC253.8907212E-545.021
CTAACGG800.045.02
CACTCGA207.0331857E-445.025
CGAATAC207.0331857E-445.032
GCGATCG207.0331857E-445.09
CGTACGT207.0331857E-445.012
CGTAATT207.0331857E-445.043
ATACGCC207.0331857E-445.041
TCGAAAC207.0331857E-445.043
GTACGTA207.0331857E-445.013