##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548021_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1110796
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88570808681342	31.0	31.0	31.0	30.0	33.0
2	31.221437599703275	31.0	31.0	33.0	30.0	34.0
3	31.390134642184524	31.0	31.0	34.0	30.0	34.0
4	35.27118300750093	37.0	35.0	37.0	33.0	37.0
5	35.09476897648173	37.0	35.0	37.0	32.0	37.0
6	35.12940990064782	37.0	35.0	37.0	32.0	37.0
7	35.49974342723596	35.0	35.0	37.0	35.0	37.0
8	35.592879340580986	37.0	35.0	37.0	35.0	37.0
9	37.43929758479505	39.0	37.0	39.0	35.0	39.0
10	36.60342223054458	39.0	35.0	39.0	32.0	39.0
11	36.19934263357088	38.0	35.0	39.0	31.0	39.0
12	35.10607348243962	37.0	34.0	39.0	30.0	39.0
13	34.44631147393401	37.0	33.0	39.0	25.0	39.0
14	35.40850525208949	37.0	33.0	40.0	27.0	41.0
15	35.843044087303156	37.0	34.0	40.0	30.0	41.0
16	36.11669469461539	37.0	34.0	40.0	31.0	41.0
17	36.06665310281996	37.0	34.0	40.0	31.0	41.0
18	35.986008231934576	37.0	34.0	40.0	30.0	41.0
19	35.885510930900004	37.0	34.0	40.0	30.0	41.0
20	35.7386225733618	36.0	34.0	40.0	30.0	41.0
21	35.56908199165283	36.0	34.0	40.0	29.0	41.0
22	35.39302626224797	36.0	34.0	40.0	29.0	41.0
23	35.386622746210826	36.0	34.0	40.0	29.0	41.0
24	35.21978923222626	36.0	34.0	40.0	29.0	41.0
25	35.05904324466419	35.0	34.0	40.0	29.0	41.0
26	34.8124255038729	35.0	33.0	40.0	27.0	41.0
27	34.880397480725534	35.0	33.0	40.0	27.0	41.0
28	34.97715602144768	36.0	34.0	40.0	27.0	41.0
29	35.05474272503682	36.0	34.0	40.0	29.0	41.0
30	34.99675367934346	36.0	34.0	40.0	28.0	41.0
31	34.85243375021156	35.0	34.0	40.0	27.0	41.0
32	34.639544074699586	35.0	33.0	40.0	27.0	41.0
33	34.49790870690928	35.0	33.0	40.0	26.0	41.0
34	34.36183601669433	35.0	33.0	40.0	25.0	41.0
35	34.204065372939766	35.0	33.0	40.0	24.0	41.0
36	34.00612893816687	35.0	33.0	40.0	23.0	41.0
37	33.87726279172773	35.0	33.0	40.0	23.0	41.0
38	33.85188999600287	35.0	33.0	40.0	23.0	41.0
39	33.87640664892564	35.0	33.0	40.0	23.0	41.0
40	33.626219395820655	35.0	32.0	40.0	23.0	41.0
41	33.62240141304074	35.0	33.0	39.0	23.0	41.0
42	33.579506948170504	35.0	33.0	39.0	23.0	41.0
43	33.48884223565803	35.0	32.0	39.0	23.0	41.0
44	33.287704492994216	35.0	32.0	39.0	23.0	41.0
45	33.15725839848181	35.0	32.0	39.0	23.0	41.0
46	33.10705926200671	35.0	32.0	39.0	22.0	41.0
47	33.05069337664162	35.0	32.0	39.0	22.0	41.0
48	33.01290515990335	35.0	32.0	39.0	22.0	41.0
49	32.91248618108096	35.0	32.0	39.0	21.0	40.0
50	32.750872347397724	35.0	31.0	38.0	20.0	40.0
51	32.3954884605274	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	17.0
15	51.0
16	156.0
17	378.0
18	861.0
19	1620.0
20	2728.0
21	4298.0
22	6469.0
23	9515.0
24	13083.0
25	17785.0
26	22155.0
27	25551.0
28	29491.0
29	34273.0
30	40226.0
31	48643.0
32	59433.0
33	76099.0
34	125605.0
35	147330.0
36	79460.0
37	95150.0
38	125940.0
39	144458.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.897035999409436	24.747298333807468	29.19356929625242	14.162096370530683
2	28.177721201732815	27.986056845721446	30.458968163371132	13.37725378917461
3	28.40701622980277	26.765850795285544	30.916838015261128	13.910294959650557
4	24.805274775926453	29.36038660564136	30.59481669001329	15.2395219284189
5	22.662126979211305	33.48130529818256	29.628302586613565	14.228265135992569
6	21.16203155214819	42.10079978681954	25.952470120526183	10.784698540506088
7	90.1821756650186	3.9709361574942657	4.776304559973209	1.070583617513927
8	90.97205967612415	3.1055207256778026	4.15989974756841	1.7625198506296385
9	87.04019459918833	5.303944198574715	5.7541618803092565	1.9016993219276987
10	50.534571604507036	29.830319878717603	10.053511175769449	9.58159734100591
11	46.835332500297085	20.71766552994429	18.293367999164563	14.15363397059406
12	42.57370390242673	21.072816250688696	21.08289911018765	15.270580736696926
13	22.04311142640053	40.80353188164164	21.503678443206493	15.649678248751345
14	16.26293216756272	42.43488453325363	24.857219507452314	16.444963791731336
15	14.694237285694223	24.807615439738708	44.55489576843993	15.943251506127137
16	17.110612569724772	20.124037176943382	43.93624031775411	18.82910993557773
17	17.113133284599513	20.58928912239511	26.149626033943225	36.14795155906215
18	22.114951800330573	23.782404689970075	32.75587956744533	21.346763942254025
19	28.12037493833251	26.17861425500272	24.060493555972474	21.640517250692294
20	30.157382633714924	24.351366047411048	24.250357401359025	21.24089391751501
21	22.527358758944036	28.929524413123563	27.935642548226674	20.607474279705727
22	25.075171318585955	24.81877860561255	24.44076139993302	25.665288675868474
23	20.521499897370894	30.745879531435115	22.905646041217288	25.826974529976702
24	21.56840680016853	23.98613246716769	36.1763996269342	18.269061105729584
25	17.864666419396542	26.67789585126342	33.150551496404375	22.30688623293566
26	18.476029802051862	34.62561982578259	24.879365788137516	22.018984584028033
27	18.66391308575112	33.81647035099154	27.378924663034436	20.140691900222905
28	14.986730236695125	30.069427689692795	37.89912819275546	17.04471388085661
29	16.82352115059831	24.617481517758435	35.86644172287261	22.692555608770647
30	18.89293803722736	30.244437322424638	31.59148934637863	19.27113529396937
31	28.746952635767503	27.08265063972142	24.320757366789223	19.84963935772185
32	29.23318052999831	27.088592324783306	26.45418240613038	17.224044739088004
33	26.69608100857403	27.955628216162104	24.93347113241315	20.414819642850713
34	20.11296403660078	28.578964994472432	27.605248848573456	23.702822120353332
35	19.797064447477304	26.59237159658479	30.011091145448848	23.599472810489054
36	30.695375208409104	24.075437794158425	26.170782033784786	19.05840496364769
37	21.00628738310185	29.963917767078748	30.06708702588054	18.962707823938867
38	20.58496789689556	31.20212892376278	24.542400224703727	23.670502954637936
39	20.88511301805192	29.957615979891898	27.74532857518392	21.411942426872262
40	24.183648482709696	25.85092132128672	25.27952927450225	24.68590092150134
41	17.62159748504676	23.358204386764086	28.63343044087303	30.386767687316123
42	22.839567301286646	24.98622609372018	25.297444355219138	26.876762249774032
43	23.16104847334704	26.04942761767237	26.24118199921498	24.548341909765607
44	20.690927947165815	30.146219467841078	27.09318362687658	22.069668958116523
45	18.608727435100594	35.670816243486655	22.007281264966743	23.71317505644601
46	23.401866769415804	30.462569184620758	25.998563192521402	20.137000853442036
47	22.14475025117123	27.20139431542786	27.69266363940814	22.96119179399278
48	23.81175301315453	23.88926499555274	29.995876830669175	22.303105160623556
49	22.00935185218528	23.122067418319837	30.85769124123601	24.010889488258872
50	20.305798724518272	30.407383533970233	27.572209478608134	21.714608262903358
51	18.83064036960882	31.49696253857594	25.079672595147983	24.592724496667255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1361.0
1	1469.5
2	1578.0
3	3856.5
4	6135.0
5	4889.5
6	3644.0
7	3747.0
8	3850.0
9	4244.5
10	4639.0
11	4844.0
12	5049.0
13	5083.0
14	5117.0
15	4888.0
16	4659.0
17	4815.5
18	4972.0
19	4959.0
20	4946.0
21	4780.5
22	4615.0
23	5376.5
24	6138.0
25	5751.5
26	6743.0
27	8121.0
28	9945.5
29	11770.0
30	12938.0
31	14106.0
32	16764.5
33	19423.0
34	21647.0
35	23871.0
36	26483.5
37	29096.0
38	34984.0
39	40872.0
40	55036.5
41	69201.0
42	83213.5
43	97226.0
44	102276.0
45	107326.0
46	104718.0
47	102110.0
48	99431.5
49	96753.0
50	93671.0
51	90589.0
52	82394.5
53	74200.0
54	66888.0
55	59576.0
56	53788.0
57	48000.0
58	42685.5
59	37371.0
60	36133.5
61	34896.0
62	30591.0
63	26286.0
64	23458.5
65	20631.0
66	16581.5
67	12532.0
68	10818.5
69	9105.0
70	7866.5
71	6628.0
72	5085.5
73	3543.0
74	3032.0
75	2047.5
76	1574.0
77	1144.0
78	714.0
79	504.5
80	295.0
81	238.5
82	182.0
83	140.5
84	99.0
85	85.5
86	72.0
87	46.0
88	20.0
89	16.5
90	13.0
91	9.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1110796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.530443289321564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69384983878916	15.955171013628233
2	6.275066070308352	2.451096440458064
3	1.9350629812813456	1.1337791345154251
4	0.9330204469090161	0.7288921170453599
5	0.5567007272464511	0.5436305991305442
6	0.36353856300200393	0.4260041572915255
7	0.2927629968269392	0.40024537747182554
8	0.23870638187736473	0.3729633163244007
9	0.21117721521252236	0.3711946163134521
>10	4.059083434698658	22.287860579995616
>50	2.8823277704722647	40.13212586426456
>100	0.5554143176874496	14.248845207440022
>500	0.002349468348931682	0.32900375536137055
>1k	9.397873395726728E-4	0.6191878207596003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3511	0.31607964018595675	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3236	0.2913226190947753	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037540646527355157	0.0
2	0.0	0.0	0.0	0.19562547938595384	0.0
3	0.0	0.0	0.0	0.2495507725991091	0.0
4	0.0	0.0	0.0	0.3977327970212352	0.0
5	0.0	0.0	0.0	0.6221664464042003	0.0
6	0.0	0.0	0.0	0.9015156698439678	0.0
7	0.0	0.0	0.0	1.026381081674763	0.0
8	0.0	0.0	0.0	1.373339479076266	0.0
9	0.0	0.0	0.0	1.4787593761590787	0.0
10	0.0	0.0	0.0	1.7365924976323286	0.0
11	0.0	0.0	0.0	2.1430577711839076	0.0
12	0.0	0.0	0.0	2.443653019996471	0.0
13	0.0	0.0	0.0	2.565727640358806	0.0
14	0.0	0.0	0.0	2.616141937853575	0.0
15	0.0	0.0	0.0	2.6955444564078372	0.0
16	0.0	0.0	0.0	2.865242582796481	0.0
17	0.0	0.0	0.0	3.0461038750589666	0.0
18	0.0	0.0	0.0	3.258294052193202	0.0
19	0.0	0.0	0.0	3.372896553462562	0.0
20	0.0	0.0	0.0	3.488039207919366	0.0
21	0.0	0.0	0.0	3.6434232748407447	0.0
22	0.0	0.0	0.0	3.7995275460120492	0.0
23	0.0	0.0	0.0	3.9688655702757303	0.0
24	0.0	0.0	0.0	4.094541211887691	0.0
25	0.0	0.0	0.0	4.199060853658097	0.0
26	0.0	0.0	0.0	4.309162078365424	0.0
27	0.0	0.0	0.0	4.407830060605187	0.0
28	0.0	0.0	0.0	4.516130774687701	0.0
29	0.0	0.0	0.0	4.6261419738637874	0.0
30	0.0	0.0	0.0	4.774594074879636	0.0
31	0.0	0.0	0.0	4.916474312114916	0.0
32	0.0	0.0	0.0	5.032427196352885	0.0
33	0.0	0.0	0.0	5.161883910276955	0.0
34	0.0	0.0	0.0	5.289720164638691	0.0
35	0.0	0.0	0.0	5.47103158455738	0.0
36	0.0	0.0	0.0	5.608950698418071	0.0
37	0.0	0.0	0.0	5.751371088840795	0.0
38	0.0	0.0	0.0	5.8968523473257015	0.0
39	0.0	0.0	0.0	6.045664550466513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	70	0.0	45.000004	6
CAAGTAG	85	0.0	45.000004	1
TAGTTCG	35	1.2118835E-7	45.000004	1
CCACTAG	35	1.2118835E-7	45.000004	20
CTATGCG	20	7.0331857E-4	45.0	1
GTACCTC	20	7.0331857E-4	45.0	33
GTACCGA	20	7.0331857E-4	45.0	27
CGTATCA	20	7.0331857E-4	45.0	22
CGTTGGC	20	7.0331857E-4	45.0	28
GACCGAA	25	3.8907212E-5	45.0	9
CGTTACC	25	3.8907212E-5	45.0	21
CTAACGG	80	0.0	45.0	2
CACTCGA	20	7.0331857E-4	45.0	25
CGAATAC	20	7.0331857E-4	45.0	32
GCGATCG	20	7.0331857E-4	45.0	9
CGTACGT	20	7.0331857E-4	45.0	12
CGTAATT	20	7.0331857E-4	45.0	43
ATACGCC	20	7.0331857E-4	45.0	41
TCGAAAC	20	7.0331857E-4	45.0	43
GTACGTA	20	7.0331857E-4	45.0	13
>>END_MODULE