FastQCFastQC Report
Thu 26 May 2016
SRR1548020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548020_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2046457
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126410.6177017156969338No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG207.03458E-445.01
TACGTCG207.03458E-445.012
CGTTTTT70750.041.9151951
CGTTAGG3300.040.9090922
GTAGCGG2100.040.7142872
TACGGGA10150.039.6798024
AGGGCGA18000.039.06
TACGTAG1100.038.8636361
GGGCGAT35200.038.6079567
TACTCGC356.250679E-638.5714345
ACGGGAT12500.038.525
AACGGGA10250.038.1951184
CGAGGGA16850.038.056384
CTACGGG4800.037.9687543
TAGGGAC27600.037.8260845
TTAGGGA35550.037.7848134
TAAGGGA39100.037.6918144
GCGATCT2150.037.6744169
GGACCGA11500.037.5652168
TATGGGA29850.037.5376854
Produced by FastQC (version 0.11.5)