##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548020_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2046457
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.873849780376524	31.0	31.0	31.0	30.0	33.0
2	31.20474898812924	31.0	31.0	33.0	30.0	34.0
3	31.32744836563876	31.0	31.0	34.0	30.0	34.0
4	35.223755006823986	37.0	35.0	37.0	33.0	37.0
5	35.07135893888804	35.0	35.0	37.0	32.0	37.0
6	35.1148594864197	37.0	35.0	37.0	32.0	37.0
7	35.560984667647546	36.0	35.0	37.0	35.0	37.0
8	35.66792949961812	37.0	35.0	37.0	35.0	37.0
9	37.49049552470441	39.0	37.0	39.0	35.0	39.0
10	36.595295185777175	39.0	35.0	39.0	32.0	39.0
11	36.14722420260968	38.0	35.0	39.0	31.0	39.0
12	35.75534399207997	37.0	35.0	39.0	30.0	39.0
13	35.53828690268107	37.0	35.0	39.0	30.0	39.0
14	36.5680373445423	38.0	35.0	40.0	30.0	41.0
15	36.78342716216368	38.0	35.0	40.0	31.0	41.0
16	36.88135494662238	38.0	35.0	40.0	31.0	41.0
17	36.829065550852036	38.0	35.0	40.0	31.0	41.0
18	36.71585085833711	38.0	35.0	40.0	31.0	41.0
19	36.66911251983306	38.0	35.0	40.0	31.0	41.0
20	36.53487808441614	38.0	35.0	40.0	31.0	41.0
21	36.41508030708683	38.0	35.0	40.0	30.0	41.0
22	36.2898770900146	38.0	34.0	40.0	30.0	41.0
23	36.23281212358725	38.0	34.0	40.0	30.0	41.0
24	36.13363339664601	38.0	34.0	40.0	30.0	41.0
25	35.98766795490939	38.0	34.0	40.0	30.0	41.0
26	35.76879553296258	38.0	34.0	40.0	29.0	41.0
27	35.81363937771475	38.0	34.0	40.0	29.0	41.0
28	35.842860612267934	38.0	34.0	40.0	29.0	41.0
29	35.85351610124229	38.0	34.0	40.0	29.0	41.0
30	35.776271380244005	38.0	34.0	40.0	29.0	41.0
31	35.685397738628275	38.0	34.0	40.0	29.0	41.0
32	35.53608553710144	38.0	34.0	40.0	29.0	41.0
33	35.36127756410225	38.0	34.0	40.0	27.0	41.0
34	35.222642840773105	38.0	34.0	40.0	27.0	41.0
35	35.07232353281794	38.0	34.0	40.0	26.0	41.0
36	34.963010217170456	38.0	34.0	40.0	25.0	41.0
37	34.91151487668688	38.0	33.0	40.0	25.0	41.0
38	34.86712840777989	38.0	33.0	40.0	25.0	41.0
39	34.84150803070868	38.0	33.0	40.0	25.0	41.0
40	34.68185160987991	37.0	33.0	40.0	24.0	41.0
41	34.637625906627896	37.0	33.0	40.0	24.0	41.0
42	34.59875140303461	37.0	33.0	40.0	24.0	41.0
43	34.55919132432297	37.0	33.0	40.0	24.0	41.0
44	34.41145257388745	37.0	33.0	40.0	23.0	41.0
45	34.29309728960833	37.0	33.0	40.0	23.0	41.0
46	34.24206274551579	37.0	33.0	40.0	23.0	41.0
47	34.19489928202743	37.0	33.0	40.0	23.0	41.0
48	34.123666414686454	37.0	33.0	40.0	23.0	41.0
49	33.98062896019804	36.0	33.0	40.0	23.0	41.0
50	33.877347532833575	36.0	33.0	40.0	23.0	41.0
51	33.55485065163842	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	7.0
14	19.0
15	64.0
16	186.0
17	490.0
18	1080.0
19	2003.0
20	3435.0
21	5805.0
22	8672.0
23	12871.0
24	18978.0
25	26679.0
26	35206.0
27	40209.0
28	45680.0
29	53152.0
30	63992.0
31	77089.0
32	94668.0
33	119356.0
34	171176.0
35	203985.0
36	176680.0
37	215634.0
38	295019.0
39	374285.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.219636180970326	25.436644894077908	28.37274372244323	13.970975202508532
2	29.175301508900503	27.050214101737787	30.10637408946291	13.6681102998988
3	27.454864675876404	26.81502714203133	32.671685747611605	13.058422434480665
4	24.721701946339454	28.76082908167628	31.900010603692134	14.617458368292127
5	22.445670737279112	34.32092636200028	30.078521073250013	13.1548818274706
6	21.49539423501202	42.089621233184964	26.571826332046065	9.843158199756946
7	91.21134722107526	2.549137362768922	5.310641757926016	0.9288736582298088
8	92.2000315667517	1.8606792129030807	4.9448388116632795	0.9944504086819317
9	87.32282183305098	5.165415154093147	5.945348472995035	1.5664145398608424
10	39.09498220583183	41.050605998562396	11.039371948689858	8.815039846915914
11	28.732682875819037	25.83938973552828	29.0171745607164	16.41075282793628
12	29.472888997912	24.223768200357984	28.84106531434572	17.462277487384295
13	22.420260968102433	30.04343604580991	29.521167559347695	18.01513542673997
14	17.73787575306982	34.29624956693446	29.725667336279237	18.240207343716484
15	17.102436063889932	28.512301993152068	37.87545988017339	16.50980206278461
16	20.692592123851124	26.58462894651586	36.421239244215734	16.301539685417286
17	20.775760252964027	25.899053828152752	29.35834957685405	23.966836342029175
18	21.285519314600794	27.731342510494965	31.917797442115813	19.065340732788425
19	24.636872409241924	27.620174770346996	28.822936421336976	18.920016399074107
20	26.547393861683876	28.483276218361787	27.882237447451864	17.087092472502476
21	23.50740816933852	27.901636828919447	30.65146250324341	17.939492498498623
22	23.239432834406003	25.674323965761314	29.731775453869787	21.3544677459629
23	20.370572164477434	29.93739912443799	29.147350762806155	20.544677948278416
24	19.40964310513243	28.33707231571443	33.92643969553233	18.32684488362081
25	19.493153288830403	29.66116561452305	31.483192659313147	19.3624884373334
26	19.57886239486097	33.91920768430512	27.660292886681713	18.841637034152196
27	18.71282905040272	32.86450680371002	30.370831148663274	18.051832997223983
28	17.458612616829967	31.42264899775563	33.71729774923197	17.401440636182436
29	18.260046509650582	28.331501712471844	33.34426279174202	20.06418898613555
30	19.465007082973155	30.549774561595967	30.891878011607375	19.093340343823495
31	23.380408188395847	29.55835377923895	28.054584093386765	19.00665393897844
32	23.93409683174384	29.547652357220304	28.648635177773095	17.869615633262757
33	22.973021177576662	29.778930121668818	28.53854246632106	18.709506234433462
34	19.872833878258863	30.22751027751866	29.589089826954584	20.31056601726789
35	20.083637232543854	29.531820116425607	29.996232513070147	20.388310137960385
36	23.492846417002657	29.988658447257873	28.303599831318227	18.21489530442125
37	20.160990433710555	32.1051456248531	29.000755940633006	18.733108000803337
38	20.17775110837902	32.14316254873667	27.477195953787447	20.201890389096864
39	20.103134343892883	31.55629461063682	28.462117699028127	19.878453346442168
40	21.56580861459586	30.15450605607643	27.753918113109634	20.525767216218078
41	18.982856712845663	29.373399978597153	28.840283475294132	22.803459833263048
42	20.553669097371703	29.989684611013086	27.713799996774913	21.7428462948403
43	21.005914123775874	29.980644596979072	28.402355876522208	20.611085402722853
44	20.464783770194046	31.101899526840782	28.5752889017458	19.858027801219375
45	19.139175658222968	33.11147021413105	26.445021810866294	21.304332316779686
46	20.484476341305975	32.365155974447546	27.187964369639822	19.962403314606657
47	20.59290764477338	30.37610856226151	28.37699497228625	20.65398882067886
48	21.3069710235788	29.019910997396963	29.539589642000784	20.133528337023453
49	20.212689540996955	28.968211890110567	30.104077437248865	20.715021131643617
50	19.463492269810704	32.30124063198005	28.307802216220523	19.927464881988723
51	19.199035210610337	32.01709100166776	27.21391165316447	21.569962134557432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1492.0
1	1739.5
2	1987.0
3	10941.5
4	19896.0
5	14848.0
6	9800.0
7	9389.5
8	8979.0
9	9925.0
10	10871.0
11	11728.5
12	12586.0
13	13411.0
14	14236.0
15	13848.5
16	13461.0
17	13145.0
18	12829.0
19	12755.0
20	12681.0
21	13527.0
22	14373.0
23	15555.0
24	16737.0
25	19395.5
26	26780.5
27	31507.0
28	36197.0
29	40887.0
30	46787.5
31	52688.0
32	57773.0
33	62858.0
34	70111.5
35	77365.0
36	85808.0
37	94251.0
38	101635.0
39	109019.0
40	123695.5
41	138372.0
42	151658.0
43	164944.0
44	168946.5
45	172949.0
46	170195.5
47	167442.0
48	162483.0
49	157524.0
50	149699.0
51	141874.0
52	126711.5
53	111549.0
54	102448.0
55	93347.0
56	82584.0
57	71821.0
58	65363.0
59	58905.0
60	52492.0
61	46079.0
62	39889.5
63	33700.0
64	26610.5
65	19521.0
66	15315.5
67	11110.0
68	8928.0
69	6746.0
70	5647.0
71	4548.0
72	3303.5
73	2059.0
74	1817.0
75	1293.5
76	1012.0
77	684.5
78	357.0
79	317.5
80	278.0
81	175.0
82	72.0
83	63.0
84	54.0
85	44.0
86	34.0
87	27.5
88	21.0
89	12.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2046457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.541342837438826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65759710197241	17.054238345323785
2	7.509504153371068	3.385486153206161
3	2.9926667537074523	2.0237618188057436
4	1.6440373180685612	1.4823523529650762
5	1.1320162464364287	1.2758583154237089
6	0.7852816086349148	1.0620781178505048
7	0.6126500879481739	0.9666968970278788
8	0.5246061878608728	0.9460262348171017
9	0.4281080729530951	0.868511775951989
>10	6.746165539982576	38.61360825082555
>50	1.8457422757572026	27.56813869241593
>100	0.12007105498405758	3.821055551583551
>500	6.658278528226872E-4	0.08710022147325065
>1k	6.658278528226872E-4	0.21267262577989002
>5k	0.0	0.0
>10k+	2.2194261760756238E-4	0.6324146465498917
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12641	0.6177017156969338	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020669869926414286	0.0
2	0.0	0.0	0.0	0.0774509310481481	0.0
3	0.0	0.0	0.0	0.1288079837494753	0.0
4	0.0	0.0	0.0	0.20132355578446065	0.0
5	0.0	0.0	0.0	0.3730349574899448	0.0
6	0.0	0.0	0.0	0.6500991713972001	0.0
7	0.0	0.0	0.0	0.8149694814012706	0.0
8	0.0	0.0	0.0	1.1599559629154192	0.0
9	0.0	0.0	0.0	1.3124634429162205	0.0
10	0.0	0.0	0.0	1.5204814955799217	0.0
11	0.0	0.0	0.0	1.7863067731205688	0.0
12	0.0	0.0	0.0	1.991637254044429	0.0
13	0.0	0.0	0.0	2.110085870360335	0.0
14	0.0	0.0	0.0	2.183090091802564	0.0
15	0.0	0.0	0.0	2.2558499885411716	0.0
16	0.0	0.0	0.0	2.3779146104706816	0.0
17	0.0	0.0	0.0	2.5401462136756354	0.0
18	0.0	0.0	0.0	2.71498497158748	0.0
19	0.0	0.0	0.0	2.8477021505949063	0.0
20	0.0	0.0	0.0	2.9723077494420846	0.0
21	0.0	0.0	0.0	3.127502801182727	0.0
22	0.0	0.0	0.0	3.3071303232855613	0.0
23	0.0	0.0	0.0	3.4993650000952865	0.0
24	0.0	0.0	0.0	3.6536316179621657	0.0
25	0.0	0.0	0.0	3.789182963531606	0.0
26	0.0	0.0	0.0	3.9240013349901806	0.0
27	4.886494072438366E-5	0.0	0.0	4.061947062655116	0.0
28	4.886494072438366E-5	0.0	0.0	4.210447617516517	0.0
29	4.886494072438366E-5	0.0	0.0	4.371604192025535	0.0
30	4.886494072438366E-5	0.0	0.0	4.570484500773777	0.0
31	4.886494072438366E-5	0.0	0.0	4.745176663863448	0.0
32	4.886494072438366E-5	0.0	0.0	4.919673367190222	0.0
33	4.886494072438366E-5	0.0	0.0	5.082979999091112	0.0
34	4.886494072438366E-5	0.0	0.0	5.26153249249801	0.0
35	4.886494072438366E-5	0.0	0.0	5.458653663380174	0.0
36	4.886494072438366E-5	0.0	0.0	5.6467348202283265	0.0
37	4.886494072438366E-5	0.0	0.0	5.839164956800949	0.0
38	4.886494072438366E-5	0.0	0.0	6.03291444677313	0.0
39	4.886494072438366E-5	0.0	0.0	6.287696247710067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	20	7.03458E-4	45.0	1
TACGTCG	20	7.03458E-4	45.0	12
CGTTTTT	7075	0.0	41.915195	1
CGTTAGG	330	0.0	40.909092	2
GTAGCGG	210	0.0	40.714287	2
TACGGGA	1015	0.0	39.679802	4
AGGGCGA	1800	0.0	39.0	6
TACGTAG	110	0.0	38.863636	1
GGGCGAT	3520	0.0	38.607956	7
TACTCGC	35	6.250679E-6	38.57143	45
ACGGGAT	1250	0.0	38.52	5
AACGGGA	1025	0.0	38.195118	4
CGAGGGA	1685	0.0	38.05638	4
CTACGGG	480	0.0	37.968754	3
TAGGGAC	2760	0.0	37.826084	5
TTAGGGA	3555	0.0	37.784813	4
TAAGGGA	3910	0.0	37.691814	4
GCGATCT	215	0.0	37.674416	9
GGACCGA	1150	0.0	37.565216	8
TATGGGA	2985	0.0	37.537685	4
>>END_MODULE