FastQCFastQC Report
Thu 26 May 2016
SRR1548019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548019_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1109464
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155771.4040113063605488No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.12510545632846132No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11840.106718199058284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG750.045.01
CTATGCG351.2118835E-745.01
TCTTGCG351.2118835E-745.01
GCCGACC207.033181E-445.014
CTACGCG406.8139343E-945.01
TCCGTCG207.033181E-445.013
CCGGTAT207.033181E-445.029
CCCGGTA253.8907187E-545.028
GGCTCGC207.033181E-445.08
CGTTTTT64000.043.3828121
TATAGCG850.042.352941
GCGATCG800.042.18759
CTCGTCC3000.041.2537
TACGGGA5250.040.7142874
GACCGAT5700.040.6578949
CGACGGT1000.040.528
CGACATA501.0822987E-940.544
TCGATAG501.0822987E-940.51
CGTTTGG1500.040.52
ACGGGAT6950.040.4676255