##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548019_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1109464
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998362272232356	31.0	31.0	33.0	30.0	33.0
2	31.31511973349293	31.0	31.0	33.0	30.0	34.0
3	31.305669224057745	31.0	31.0	34.0	30.0	34.0
4	35.28920812212023	37.0	35.0	37.0	33.0	37.0
5	35.22266517886114	37.0	35.0	37.0	33.0	37.0
6	35.295887022922784	37.0	35.0	37.0	33.0	37.0
7	35.67640049609541	36.0	35.0	37.0	35.0	37.0
8	35.80236943244666	37.0	35.0	37.0	35.0	37.0
9	37.66067939112941	39.0	37.0	39.0	35.0	39.0
10	36.87807535891205	39.0	37.0	39.0	32.0	39.0
11	36.470230669945124	38.0	35.0	39.0	32.0	39.0
12	36.17308718444222	38.0	35.0	39.0	32.0	39.0
13	36.02595217149903	38.0	35.0	39.0	31.0	39.0
14	37.09840066915195	39.0	36.0	41.0	31.0	41.0
15	37.269553586236235	39.0	36.0	41.0	32.0	41.0
16	37.359048152981984	39.0	35.0	41.0	32.0	41.0
17	37.30314728553608	39.0	35.0	41.0	32.0	41.0
18	37.180607031864035	39.0	36.0	40.0	32.0	41.0
19	37.08063443248271	38.0	36.0	40.0	32.0	41.0
20	36.90530382238631	38.0	35.0	40.0	31.0	41.0
21	36.79650714218758	38.0	35.0	40.0	31.0	41.0
22	36.70040578153054	38.0	35.0	40.0	31.0	41.0
23	36.61074807294333	38.0	35.0	40.0	31.0	41.0
24	36.50898722265887	38.0	35.0	40.0	31.0	41.0
25	36.34448887030133	38.0	35.0	40.0	30.0	41.0
26	36.133825883489685	38.0	35.0	40.0	30.0	41.0
27	36.14817966153025	38.0	35.0	40.0	30.0	41.0
28	36.1680613341217	38.0	35.0	40.0	30.0	41.0
29	36.11129518398074	38.0	35.0	40.0	30.0	41.0
30	36.00549093976911	38.0	35.0	40.0	30.0	41.0
31	35.8901496578528	38.0	34.0	40.0	30.0	41.0
32	35.69519605863732	38.0	34.0	40.0	29.0	41.0
33	35.4936744229646	38.0	34.0	40.0	29.0	41.0
34	35.22894659042565	38.0	34.0	40.0	27.0	41.0
35	35.00164764246519	38.0	34.0	40.0	25.0	41.0
36	34.87837640518304	38.0	34.0	40.0	25.0	41.0
37	34.8109285204387	38.0	34.0	40.0	24.0	41.0
38	34.723211388562405	37.0	34.0	40.0	24.0	41.0
39	34.68332816567279	37.0	34.0	40.0	24.0	41.0
40	34.523064290504244	37.0	33.0	40.0	23.0	41.0
41	34.463196642703146	37.0	33.0	40.0	23.0	41.0
42	34.42087170020839	37.0	33.0	40.0	23.0	41.0
43	34.39854470266723	37.0	33.0	40.0	23.0	41.0
44	34.26714161072373	37.0	33.0	40.0	23.0	41.0
45	34.15320641318691	37.0	33.0	40.0	23.0	41.0
46	34.09487644484183	37.0	33.0	40.0	23.0	41.0
47	34.05103184961387	36.0	33.0	40.0	23.0	41.0
48	33.95089610839108	36.0	33.0	40.0	23.0	41.0
49	33.824557624222145	36.0	33.0	40.0	22.0	41.0
50	33.721873805729615	36.0	33.0	40.0	22.0	41.0
51	33.43612050503667	35.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	13.0
15	19.0
16	67.0
17	215.0
18	422.0
19	856.0
20	1496.0
21	2542.0
22	4041.0
23	6432.0
24	10048.0
25	15232.0
26	20034.0
27	21734.0
28	22609.0
29	25313.0
30	30350.0
31	37254.0
32	46899.0
33	61240.0
34	88017.0
35	112054.0
36	113522.0
37	128034.0
38	161797.0
39	199195.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.786397756033544	24.945739564330164	29.18454316679045	15.083319512845842
2	32.59916500219926	25.69006294931607	28.844559174520306	12.866212873964363
3	27.342662763280284	25.811292660239538	33.944319058572425	12.901725517907748
4	23.871346884621765	28.18433045146125	33.67860516429555	14.265717499621438
5	21.07278830137796	32.61313571238003	32.88101281339458	13.43306317284743
6	19.765490362913983	41.30201610867951	29.094679953563162	9.837813574843347
7	88.72689875471399	2.4313542395246714	7.893992053820583	0.9477549519407569
8	89.582897687532	1.9102918165889116	7.49893642335398	1.0078740725251112
9	84.83646157063231	5.279035642436348	8.416136080125177	1.4683667068061694
10	34.63275960283524	44.96585738699048	12.506760021055213	7.894622989119069
11	25.81480787118825	27.312468002567	31.176315770498185	15.696408355746557
12	26.62907494069208	25.913414044980275	30.62226444481299	16.83524656951465
13	21.434584628252924	30.678778220834563	31.44987128919911	16.436765861713404
14	17.113759436989394	34.65285939877274	30.94656518823504	17.286815976002824
15	16.161948472415506	31.4091308956397	36.50916118053402	15.919759451410773
16	20.083842287807446	29.995385158959643	34.87927503731532	15.041497515917596
17	19.848954089542335	29.4813531579213	29.806915771940325	20.862776980596035
18	20.392279515153263	31.134313506341798	31.889002256945698	16.58440472155924
19	22.17980934937952	32.68262872882761	28.119163848489	17.018398073303867
20	24.089199829827738	32.116319231628964	27.824787464938023	15.969693473605274
21	22.066691663722303	31.79724623782295	30.218736254623856	15.917325843830895
22	21.55103725763071	28.786603260673623	30.468406365596362	19.193953116099305
23	18.162644303916124	33.354124153645365	30.018098829705153	18.465132712733357
24	17.843571310110107	32.98728034438251	33.19557912649712	15.973569219010262
25	18.855050727197998	34.05617487363267	30.462908215138118	16.625866184031207
26	17.955066590714075	36.99209708471838	27.704368956541174	17.348467368026363
27	16.852371956187852	36.03460770245812	30.383590634756963	16.72942970659706
28	15.986638593050337	34.47673831688095	32.890206442029665	16.646416648039054
29	16.590894341772245	32.59240498114405	32.56338195741367	18.253318719670037
30	18.27296784753719	34.08618936711781	31.153782366980813	16.487060418364184
31	21.53751721552029	33.692756141704464	28.10870834925694	16.66101829351831
32	21.12542633199455	34.086549901574095	28.236067145937138	16.55195662049422
33	20.2278758030905	33.87104043033393	28.10357073325498	17.797513033320595
34	17.832845410035837	34.293496679477656	28.81950202980899	19.054155880677516
35	17.957950866364296	33.81065090890736	29.33858151323522	18.89281671149312
36	21.21709221750323	34.41112104583835	27.12571115421501	17.246075582443414
37	18.202212960492634	35.39736305098678	28.205782251609783	18.194641736910796
38	18.25836620205793	36.57495871880476	26.679369497342858	18.487305581794452
39	18.029967624005828	35.58105535646042	27.083708890058624	19.305268129475134
40	20.448162355876352	34.073029859463674	27.18429800336018	18.294509781299798
41	17.727479215188595	33.11608127888782	28.19487608430738	20.9615634216162
42	18.323262404187968	34.46114520164692	26.846837752283985	20.36875464188112
43	18.671268288110294	34.50251653050482	26.952564481587505	19.87365069979738
44	18.769874461902326	35.2143918144257	27.41918620162529	18.596547522046684
45	17.88773678100416	36.80452903383976	25.97614704037265	19.331587144783427
46	19.555479042132056	36.18828551444662	26.174440991325543	18.081794452095785
47	18.710296143002385	34.89270494581167	27.068926977351225	19.328071933834718
48	18.803854834406525	34.732717780838314	28.13034041663362	18.333086968121542
49	18.962940663239188	33.7215988982067	27.910414398304045	19.405046040250067
50	17.86475270941644	35.94420368754642	27.629738324091633	18.561305278945508
51	17.606069237036984	35.883633898891716	26.742553160805578	19.767743703265722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	779.0
1	903.5
2	1028.0
3	10813.0
4	20598.0
5	14671.0
6	8744.0
7	8668.0
8	8592.0
9	10635.0
10	12678.0
11	15129.0
12	17580.0
13	18596.0
14	19612.0
15	18882.0
16	18152.0
17	16567.0
18	14982.0
19	13683.5
20	12385.0
21	11615.0
22	10845.0
23	10708.0
24	10571.0
25	11710.0
26	14556.5
27	16264.0
28	19646.5
29	23029.0
30	26046.0
31	29063.0
32	32639.0
33	36215.0
34	40056.5
35	43898.0
36	47985.5
37	52073.0
38	55111.5
39	58150.0
40	64176.0
41	70202.0
42	76407.0
43	82612.0
44	84024.5
45	85437.0
46	86560.0
47	87683.0
48	86145.5
49	84608.0
50	77415.5
51	70223.0
52	62936.0
53	55649.0
54	48560.0
55	41471.0
56	37519.0
57	33567.0
58	29149.5
59	24732.0
60	21134.0
61	17536.0
62	14170.5
63	10805.0
64	8819.0
65	6833.0
66	5129.0
67	3425.0
68	2847.5
69	2270.0
70	1827.0
71	1384.0
72	1420.5
73	1457.0
74	1116.0
75	557.5
76	340.0
77	260.5
78	181.0
79	130.0
80	79.0
81	59.5
82	40.0
83	31.0
84	22.0
85	20.5
86	19.0
87	15.5
88	12.0
89	9.5
90	7.0
91	5.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1109464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.611641952546808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.07422224345619	22.46772865615875
2	9.597284314138998	6.067718309107116
3	4.232073533117877	4.013483797373162
4	2.5066387645761576	3.1695586852062307
5	1.6872418538253524	2.6668242685239187
6	1.2345848119559981	2.341635182136321
7	1.0235187193539215	2.2648575101561788
8	0.8181505637141838	2.069046614672569
9	0.7074051340381855	2.0126014031347657
>10	6.782224660670876	43.15640232876701
>50	0.3003784831109082	6.1157907119671755
>100	0.03395519528882597	1.6460320128508077
>500	0.001160861376232776	0.24540940984220022
>1k	8.70646032174582E-4	0.33384853922084734
>5k	0.0	0.0
>10k+	2.90215344058194E-4	1.429062570882973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15577	1.4040113063605488	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.12510545632846132	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1184	0.106718199058284	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022533403517374154	0.0
2	0.0	0.0	0.0	0.07625303750279414	0.0
3	0.0	0.0	0.0	0.12726866306612922	0.0
4	0.0	0.0	0.0	0.19946568793579603	0.0
5	0.0	0.0	0.0	0.39181082036010184	0.0
6	0.0	0.0	0.0	0.7485596648471694	0.0
7	0.0	0.0	0.0	1.0010239178558296	0.0
8	0.0	0.0	0.0	1.417080680400626	0.0
9	0.0	0.0	0.0	1.6496254046999272	0.0
10	0.0	0.0	0.0	1.8896512189669967	0.0
11	0.0	0.0	0.0	2.14689255352134	0.0
12	0.0	0.0	0.0	2.336894211979839	0.0
13	0.0	0.0	0.0	2.46389247420376	0.0
14	0.0	0.0	0.0	2.5515023470793103	0.0
15	0.0	0.0	0.0	2.633523935882552	0.0
16	0.0	0.0	0.0	2.7462810870834926	0.0
17	0.0	0.0	0.0	2.89536208475444	0.0
18	0.0	0.0	0.0	3.0553492497277963	0.0
19	0.0	6.309352984864763E-4	0.0	3.1805448396703273	0.0
20	0.0	6.309352984864763E-4	0.0	3.3026758867344954	0.0
21	0.0	6.309352984864763E-4	0.0	3.443104057454771	0.0
22	0.0	6.309352984864763E-4	0.0	3.597953606426166	0.0
23	0.0	6.309352984864763E-4	0.0	3.770379210141113	0.0
24	0.0	6.309352984864763E-4	0.0	3.9163956649336975	0.0
25	0.0	6.309352984864763E-4	0.0	4.040689918735533	0.0
26	0.0	6.309352984864763E-4	0.0	4.164443370852952	0.0
27	0.0	6.309352984864763E-4	0.0	4.297120050763252	0.0
28	0.0	6.309352984864763E-4	0.0	4.436376484500624	0.0
29	0.0	6.309352984864763E-4	0.0	4.609523157128127	0.0
30	0.0	6.309352984864763E-4	0.0	4.7960096046379155	0.0
31	0.0	6.309352984864763E-4	0.0	4.965100264632291	0.0
32	0.0	6.309352984864763E-4	0.0	5.139959475927115	0.0
33	0.0	6.309352984864763E-4	0.0	5.304994123288362	0.0
34	0.0	6.309352984864763E-4	0.0	5.486703489252468	0.0
35	0.0	6.309352984864763E-4	0.0	5.680851293958163	0.0
36	0.0	6.309352984864763E-4	0.0	5.862109991851922	0.0
37	0.0	6.309352984864763E-4	0.0	6.047334568764737	0.0
38	0.0	6.309352984864763E-4	0.0	6.260230165196888	0.0
39	0.0	6.309352984864763E-4	0.0	6.555598018502629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	75	0.0	45.0	1
CTATGCG	35	1.2118835E-7	45.0	1
TCTTGCG	35	1.2118835E-7	45.0	1
GCCGACC	20	7.033181E-4	45.0	14
CTACGCG	40	6.8139343E-9	45.0	1
TCCGTCG	20	7.033181E-4	45.0	13
CCGGTAT	20	7.033181E-4	45.0	29
CCCGGTA	25	3.8907187E-5	45.0	28
GGCTCGC	20	7.033181E-4	45.0	8
CGTTTTT	6400	0.0	43.382812	1
TATAGCG	85	0.0	42.35294	1
GCGATCG	80	0.0	42.1875	9
CTCGTCC	300	0.0	41.25	37
TACGGGA	525	0.0	40.714287	4
GACCGAT	570	0.0	40.657894	9
CGACGGT	100	0.0	40.5	28
CGACATA	50	1.0822987E-9	40.5	44
TCGATAG	50	1.0822987E-9	40.5	1
CGTTTGG	150	0.0	40.5	2
ACGGGAT	695	0.0	40.467625	5
>>END_MODULE