FastQCFastQC Report
Thu 26 May 2016
SRR1548018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548018_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1591052
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43520.27352971493075023No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24410.1534205041695683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT302.166029E-645.00000443
CGTTGAC253.8914834E-545.00000425
TAACGCG253.8914834E-545.0000041
TATAGCG1150.045.0000041
CGGTCTA2000.045.00000431
TTCGATC253.8914834E-545.00000438
TTATCCG207.0341054E-445.01
CGTACCG207.0341054E-445.045
GGCGAAC207.0341054E-445.010
ATCGCCG207.0341054E-445.011
CGACGGT2300.043.0434828
CGTAAGG2150.042.9069752
TCACGAC2300.041.0869625
CACGACG2200.040.9090926
ACGGTCT2200.040.9090930
CGCATAC501.0822987E-940.50000426
TCGTAAG950.040.2631571
ATTAGCG1400.040.1785741
AATCGAG451.9290383E-840.01
GACGGTC2500.039.629