##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548018_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1591052
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.986069594205595	31.0	31.0	33.0	30.0	33.0
2	31.34513642545938	31.0	31.0	33.0	30.0	34.0
3	31.534362170438175	31.0	31.0	34.0	30.0	34.0
4	35.398772007451676	37.0	35.0	37.0	33.0	37.0
5	35.23507276946322	37.0	35.0	37.0	33.0	37.0
6	35.267404836548394	37.0	35.0	37.0	32.0	37.0
7	35.59447334216606	36.0	35.0	37.0	35.0	37.0
8	35.67900609156709	37.0	35.0	37.0	35.0	37.0
9	37.517101892332875	39.0	37.0	39.0	35.0	39.0
10	36.72959086189515	39.0	37.0	39.0	32.0	39.0
11	36.366170307444385	38.0	35.0	39.0	32.0	39.0
12	35.63196614566966	37.0	35.0	39.0	31.0	39.0
13	35.22181613171662	37.0	35.0	39.0	30.0	39.0
14	36.1831876016623	38.0	35.0	40.0	30.0	41.0
15	36.46863207487876	38.0	35.0	40.0	30.0	41.0
16	36.646530723068764	38.0	35.0	40.0	31.0	41.0
17	36.58926295306502	38.0	35.0	40.0	31.0	41.0
18	36.50874390026222	38.0	35.0	40.0	31.0	41.0
19	36.46575096225642	38.0	35.0	40.0	31.0	41.0
20	36.350258193949664	38.0	35.0	40.0	30.0	41.0
21	36.2010506256238	38.0	34.0	40.0	30.0	41.0
22	36.08475147261058	38.0	34.0	40.0	30.0	41.0
23	36.054049773357505	38.0	34.0	40.0	30.0	41.0
24	35.94532485424738	38.0	34.0	40.0	30.0	41.0
25	35.80368397764498	37.0	34.0	40.0	30.0	41.0
26	35.57753297818047	37.0	34.0	40.0	29.0	41.0
27	35.64075529900971	37.0	34.0	40.0	29.0	41.0
28	35.69638641603166	37.0	34.0	40.0	29.0	41.0
29	35.70994788353869	37.0	34.0	40.0	30.0	41.0
30	35.666494244059905	37.0	34.0	40.0	29.0	41.0
31	35.545552879478485	37.0	34.0	40.0	29.0	41.0
32	35.40399622388206	37.0	34.0	40.0	29.0	41.0
33	35.266627992045514	37.0	34.0	40.0	28.0	41.0
34	35.11302144744484	37.0	34.0	40.0	27.0	41.0
35	34.97429750881807	37.0	34.0	40.0	26.0	41.0
36	34.89790905639791	37.0	33.0	40.0	26.0	41.0
37	34.83380555758077	37.0	33.0	40.0	26.0	41.0
38	34.774417806583315	37.0	33.0	40.0	26.0	41.0
39	34.76718045670412	37.0	33.0	40.0	25.0	41.0
40	34.56038771831467	37.0	33.0	40.0	24.0	41.0
41	34.500284717281396	36.0	33.0	40.0	24.0	41.0
42	34.47331639694994	36.0	33.0	40.0	24.0	41.0
43	34.438985652260264	36.0	33.0	40.0	24.0	41.0
44	34.27862508579229	36.0	33.0	40.0	24.0	41.0
45	34.15238973961882	36.0	33.0	40.0	23.0	41.0
46	34.118442389060824	36.0	33.0	40.0	23.0	41.0
47	34.06649625530781	36.0	33.0	40.0	23.0	41.0
48	33.989143660923716	35.0	33.0	40.0	23.0	41.0
49	33.86848387104884	36.0	33.0	40.0	24.0	41.0
50	33.72272810693805	35.0	33.0	39.0	23.0	41.0
51	33.3838906585077	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	10.0
14	20.0
15	54.0
16	171.0
17	431.0
18	866.0
19	1705.0
20	2911.0
21	4575.0
22	6894.0
23	9837.0
24	14067.0
25	19397.0
26	24777.0
27	29992.0
28	34671.0
29	41665.0
30	50527.0
31	61168.0
32	76669.0
33	98354.0
34	155871.0
35	179761.0
36	130203.0
37	161773.0
38	218683.0
39	265969.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37515052933531	24.6098179066429	29.821526889127448	12.193504674894347
2	30.737713160852064	27.509723126585428	28.886170910818755	12.86639280174375
3	29.728632376565944	26.201092107611817	29.97834137413485	14.091934141687387
4	26.544889796185167	28.77674645454705	29.5024926903709	15.175871058896881
5	23.466863433753264	33.60675829576909	28.74217813119873	14.184200139278918
6	22.476826653057223	41.75300367304148	25.6297091484125	10.140460525488797
7	90.75668174264575	3.314913654613426	4.715433562196584	1.2129710405442438
8	91.0321598539834	2.8304543157608926	4.476409319117162	1.6609765111385424
9	85.90555179843274	5.596485847099906	6.072083124875868	2.4258792295914904
10	43.42617337459744	35.503867881125196	10.982293476266019	10.087665268011353
11	39.64986688052936	23.443608379864393	21.691434346583264	15.215090393022981
12	37.91447419694642	21.956730515407415	24.11366818934893	16.015127098297228
13	25.430218496944164	32.021706393002866	25.047515731729696	17.50055937832327
14	18.975872567332807	34.95473435186279	27.668988819975716	18.400404260828683
15	18.4021641027446	26.38279578543002	39.25578799435845	15.959252117466935
16	22.191040896212066	22.94155062185271	37.82459655624078	17.042811925694444
17	22.437795873422115	22.33566219080206	26.80553495423154	28.421006981544288
18	23.454418837347866	25.193205501768638	31.30739912963247	20.04497653125102
19	27.95955129059264	26.963480766184887	25.550139153214353	19.52682879000812
20	30.50956222675312	26.5215090393023	24.882027740136714	18.08690099380787
21	26.26991449682349	27.245746839198215	27.988525830708237	18.49581283327006
22	25.194211125720596	23.472017256507016	27.65912113494719	23.6746504828252
23	22.558910708135247	28.65110631205014	26.91709636140114	21.872886618413478
24	22.67996269135138	25.566354839439565	32.44054876899058	19.313133700218472
25	21.38541040770509	26.27802233993609	30.910680480587686	21.42588677177113
26	20.024738349218	32.37656594504768	25.854717507661597	21.74397819807272
27	20.540560585072015	31.907253816971416	28.360543841433216	19.191641756523357
28	17.561965290889297	29.98946608910331	33.18867013774534	19.25989848226205
29	19.646246634302337	26.752111181784127	32.75254360008347	20.84909858383007
30	21.61104728192416	28.628542624628235	29.67319735621463	20.087212737232978
31	26.816848223690993	27.39036813378821	25.756103508873373	20.036680133647423
32	28.46713998034005	26.785171069204527	26.61396359138482	18.133725359070603
33	27.387665519417343	27.729766217571772	25.261650781998323	19.62091748101256
34	21.689737356164347	27.287794490689176	29.03135786888172	21.99111028426475
35	20.961351357466633	27.70952803553875	29.82184114661243	21.507279460382186
36	27.4462431146185	26.983153284744937	26.17903123216589	19.391572368470673
37	22.837845651807733	30.771275860248437	27.024509569768934	19.366368918174892
38	22.29110047943122	30.70169925307281	25.05977177364411	21.947428493851866
39	21.73455047352318	29.360385455660783	27.35240582960205	21.55265824121399
40	24.597121904249516	25.57867373285097	27.237010481115636	22.587193881783875
41	19.408228015174867	25.3199769712115	28.638347458159757	26.633447555453877
42	22.672671917699734	26.638538526710633	26.019514132787613	24.66927542280202
43	22.22108391177661	26.183053727973693	27.016841687135308	24.579020673114393
44	20.86374298263036	28.437788331242476	28.350424750416703	22.34804393571046
45	20.804725426950217	31.41782920985612	24.692970437169873	23.084474926023788
46	22.12473256688028	30.179340461531112	26.715405907537903	20.980521064050702
47	22.43006513929149	27.44599170863052	27.365981752953395	22.757961399124603
48	22.852112941626043	25.54731083584949	29.97167911545317	21.628897107071296
49	22.19179511417603	24.614846026402656	30.916714224299398	22.27664463512192
50	21.311622750230665	29.71071969992181	27.616947780462237	21.360709769385284
51	20.104056938428158	30.3302469058208	26.17375170641814	23.391944449332893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1047.0
1	1607.5
2	2168.0
3	5316.5
4	8465.0
5	6602.5
6	4740.0
7	4675.5
8	4611.0
9	4904.0
10	5197.0
11	5315.5
12	5434.0
13	5332.0
14	5230.0
15	5190.5
16	5151.0
17	5794.0
18	6437.0
19	5992.0
20	5547.0
21	6840.5
22	8134.0
23	8188.5
24	8243.0
25	9324.5
26	12811.5
27	15217.0
28	17681.5
29	20146.0
30	24464.0
31	28782.0
32	32797.0
33	36812.0
34	42524.0
35	48236.0
36	50105.0
37	51974.0
38	60206.5
39	68439.0
40	81782.5
41	95126.0
42	110481.5
43	125837.0
44	130701.5
45	135566.0
46	133141.0
47	130716.0
48	127685.5
49	124655.0
50	116846.0
51	109037.0
52	102950.0
53	96863.0
54	91995.0
55	87127.0
56	82121.0
57	77115.0
58	73156.5
59	69198.0
60	64036.0
61	58874.0
62	52577.0
63	46280.0
64	39027.0
65	31774.0
66	26080.5
67	20387.0
68	16187.5
69	11988.0
70	9470.0
71	6952.0
72	6166.0
73	5380.0
74	4475.0
75	2763.0
76	1956.0
77	1581.0
78	1206.0
79	815.5
80	425.0
81	348.5
82	272.0
83	199.5
84	127.0
85	118.5
86	110.0
87	73.0
88	36.0
89	25.0
90	14.0
91	24.5
92	35.0
93	21.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1591052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.305006837474437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19582270180923	15.288758987578408
2	6.566671849874234	2.5353928992254597
3	2.1229740192316764	1.2295208387114431
4	1.104039818462584	0.8525398497705687
5	0.7056806729482892	0.6811585108170143
6	0.5488746023571699	0.6357616770852735
7	0.42086111640474866	0.5687308710874558
8	0.3462099065478682	0.5346867690486385
9	0.3033510210722997	0.5270574182360038
>10	5.612914803631479	28.776633977941124
>50	2.562769273240858	34.89077755596487
>100	0.5071731188394918	12.74686921259539
>500	0.0016606847375219076	0.2181971626279756
>1k	9.964108425131444E-4	0.5139142693103803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4352	0.27352971493075023	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2441	0.1534205041695683	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02224942993692224	0.0
2	0.0	0.0	0.0	0.09842544429723227	0.0
3	0.0	0.0	0.0	0.14499840357197627	0.0
4	0.0	0.0	0.0	0.2861000143301413	0.0
5	0.0	0.0	0.0	0.4910587460372131	0.0
6	0.0	0.0	0.0	0.752395270550554	0.0
7	0.0	0.0	0.0	0.8559116861045396	0.0
8	0.0	0.0	0.0	1.1134771207980632	0.0
9	0.0	0.0	0.0	1.2008407016238314	0.0
10	0.0	0.0	0.0	1.3738080213594528	0.0
11	0.0	0.0	0.0	1.688254060835221	0.0
12	0.0	0.0	0.0	1.9195475697840172	0.0
13	0.0	0.0	0.0	2.03035475898965	0.0
14	0.0	0.0	0.0	2.082018689521147	0.0
15	0.0	0.0	0.0	2.1545493170556336	0.0
16	0.0	0.0	0.0	2.295085264340826	0.0
17	0.0	0.0	0.0	2.44611741162451	0.0
18	0.0	0.0	0.0	2.6168849289652383	0.0
19	0.0	0.0	0.0	2.727126454697898	0.0
20	0.0	0.0	0.0	2.8479898834230433	0.0
21	0.0	0.0	0.0	3.002290308550569	0.0
22	0.0	0.0	0.0	3.1836797288837824	0.0
23	0.0	0.0	0.0	3.3531902162845713	0.0
24	0.0	0.0	0.0	3.48926370728298	0.0
25	0.0	0.0	0.0	3.6071731156492683	0.0
26	0.0	0.0	0.0	3.7246425635365785	0.0
27	0.0	0.0	0.0	3.8472658341776382	0.0
28	0.0	0.0	0.0	3.9715860952376163	0.0
29	0.0	0.0	0.0	4.111493527552839	0.0
30	0.0	0.0	0.0	4.284963659264436	0.0
31	6.285149699695547E-5	0.0	0.0	4.440521114331901	0.0
32	6.285149699695547E-5	0.0	0.0	4.58463959694592	0.0
33	6.285149699695547E-5	0.0	0.0	4.733597644828705	0.0
34	6.285149699695547E-5	0.0	0.0	4.891040644806078	0.0
35	6.285149699695547E-5	0.0	0.0	5.0764525609470965	0.0
36	6.285149699695547E-5	0.0	0.0	5.241186334576117	0.0
37	6.285149699695547E-5	0.0	0.0	5.415913496227653	0.0
38	6.285149699695547E-5	0.0	0.0	5.604782244703505	0.0
39	6.285149699695547E-5	0.0	0.0	5.880826019514132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	30	2.166029E-6	45.000004	43
CGTTGAC	25	3.8914834E-5	45.000004	25
TAACGCG	25	3.8914834E-5	45.000004	1
TATAGCG	115	0.0	45.000004	1
CGGTCTA	200	0.0	45.000004	31
TTCGATC	25	3.8914834E-5	45.000004	38
TTATCCG	20	7.0341054E-4	45.0	1
CGTACCG	20	7.0341054E-4	45.0	45
GGCGAAC	20	7.0341054E-4	45.0	10
ATCGCCG	20	7.0341054E-4	45.0	11
CGACGGT	230	0.0	43.04348	28
CGTAAGG	215	0.0	42.906975	2
TCACGAC	230	0.0	41.08696	25
CACGACG	220	0.0	40.90909	26
ACGGTCT	220	0.0	40.90909	30
CGCATAC	50	1.0822987E-9	40.500004	26
TCGTAAG	95	0.0	40.263157	1
ATTAGCG	140	0.0	40.178574	1
AATCGAG	45	1.9290383E-8	40.0	1
GACGGTC	250	0.0	39.6	29
>>END_MODULE