FastQCFastQC Report
Thu 26 May 2016
SRR1548017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548017_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1679297
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165550.9858291892381157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG406.8175723E-945.0000041
CGGTCTA406.8175723E-945.00000431
CTATGCG551.8189894E-1245.01
CGTTTTT89800.042.5445441
CGGGTAC501.0822987E-940.56
TACGGGA8850.040.169494
CACGACC1800.040.027
CGAGGGA13650.039.230774
CGACAGG1850.038.918922
CGTTAGG2950.038.8983042
TAGGGAC23700.038.734185
GGGCGAT30850.038.7277157
ACGGGAT13050.038.620695
ACGTCGG356.2500094E-638.571432
TAGGGCG6200.038.467745
TGGGCGA8600.038.45936
AGGGCGA15050.038.4219256
ACGGGAC6100.038.3606575
GGCGATT9250.038.189198
TTAGGGA31500.038.1428574