##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548017_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1679297
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38673742643499	31.0	31.0	33.0	30.0	33.0
2	31.775747232324	31.0	31.0	34.0	30.0	34.0
3	31.851273479319026	31.0	31.0	34.0	30.0	34.0
4	35.772554825025	37.0	35.0	37.0	35.0	37.0
5	35.75265899956946	37.0	35.0	37.0	35.0	37.0
6	35.81170334967549	37.0	35.0	37.0	35.0	37.0
7	35.91822470950642	37.0	35.0	37.0	35.0	37.0
8	36.03475144658747	37.0	35.0	37.0	35.0	37.0
9	37.97062044415014	39.0	38.0	39.0	35.0	39.0
10	37.43366480140202	39.0	37.0	39.0	35.0	39.0
11	37.07354267887098	39.0	37.0	39.0	33.0	39.0
12	36.82478977810358	39.0	35.0	39.0	33.0	39.0
13	36.71802069556487	39.0	35.0	39.0	33.0	39.0
14	37.924498763470666	40.0	37.0	41.0	33.0	41.0
15	38.04851732600011	40.0	37.0	41.0	33.0	41.0
16	38.130961348707224	40.0	37.0	41.0	33.0	41.0
17	38.07858347868185	40.0	36.0	41.0	33.0	41.0
18	37.947269601505866	39.0	36.0	41.0	33.0	41.0
19	37.85188504475385	39.0	36.0	41.0	34.0	41.0
20	37.697060138855726	39.0	35.0	41.0	33.0	41.0
21	37.61080142464377	39.0	35.0	41.0	33.0	41.0
22	37.50665248612961	39.0	35.0	41.0	33.0	41.0
23	37.414299555111455	39.0	35.0	41.0	33.0	41.0
24	37.326035239746155	39.0	35.0	41.0	33.0	41.0
25	37.203550056958356	39.0	35.0	41.0	33.0	41.0
26	37.060050723606366	39.0	35.0	41.0	32.0	41.0
27	37.13794760545633	39.0	35.0	41.0	32.0	41.0
28	37.17528406231894	39.0	35.0	41.0	33.0	41.0
29	37.16042486826333	39.0	35.0	41.0	33.0	41.0
30	37.09638318891774	39.0	35.0	41.0	32.0	41.0
31	37.00169058838311	39.0	35.0	41.0	32.0	41.0
32	36.8290385798343	39.0	35.0	41.0	31.0	41.0
33	36.61680572287094	39.0	35.0	41.0	31.0	41.0
34	36.38706137151439	39.0	35.0	41.0	30.0	41.0
35	36.14342311098037	39.0	35.0	41.0	30.0	41.0
36	36.07027107176396	39.0	35.0	41.0	30.0	41.0
37	36.01926579991508	39.0	35.0	41.0	29.0	41.0
38	35.95176314850798	39.0	35.0	41.0	29.0	41.0
39	35.910988348100425	39.0	35.0	41.0	29.0	41.0
40	35.776349269962374	39.0	35.0	40.0	28.0	41.0
41	35.700951648219466	38.0	35.0	40.0	28.0	41.0
42	35.672655283728844	38.0	35.0	40.0	28.0	41.0
43	35.63907396964325	38.0	35.0	40.0	28.0	41.0
44	35.50037366826714	38.0	35.0	40.0	27.0	41.0
45	35.39624438083317	38.0	35.0	40.0	27.0	41.0
46	35.340706855309094	38.0	35.0	40.0	26.0	41.0
47	35.31609953450759	38.0	35.0	40.0	26.0	41.0
48	35.23713792140401	38.0	34.0	40.0	26.0	41.0
49	35.12624092105208	38.0	34.0	40.0	26.0	41.0
50	35.022150935778484	38.0	34.0	40.0	26.0	41.0
51	34.71301800693981	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	6.0
15	27.0
16	78.0
17	142.0
18	325.0
19	759.0
20	1318.0
21	2244.0
22	3802.0
23	6080.0
24	10312.0
25	17076.0
26	23774.0
27	25620.0
28	25402.0
29	26246.0
30	29830.0
31	36541.0
32	47859.0
33	67059.0
34	113646.0
35	152586.0
36	139977.0
37	195227.0
38	307530.0
39	445762.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.6772381538227	25.197865535399632	30.54843782844845	15.576458482329214
2	32.24194409922724	26.20352445100539	28.703320496612573	12.851210953154801
3	27.233300601382602	25.742498200139703	33.786459452973475	13.23774174550422
4	24.216978890571472	28.38693810564778	33.28005707150075	14.116025932279996
5	22.034279820663052	32.31524858318689	32.01297924071799	13.637492355432066
6	21.139977026100805	40.59430821349648	28.379315868485445	9.886398891917272
7	89.52996402661351	2.322043093032382	7.277628674379814	0.8703642059742858
8	90.63227052748859	1.6413415851990447	6.8988987653762255	0.8274891219361434
9	85.62714040458597	5.057771198305005	7.857156893628703	1.4579315034803255
10	35.98053232989757	43.3801763476026	12.415790655256338	8.223500667243496
11	25.620066015719672	25.607560782875215	32.68992917869799	16.082444022707122
12	27.627870471989173	23.61982424788468	31.62394740179968	17.128357878326465
13	22.406816661972243	28.126888811210883	31.893405395233838	17.57288913158304
14	17.631187336129344	31.84135980710976	31.888224655912563	18.639228200848333
15	17.300334604301682	28.44880923386393	37.85464989218703	16.396206269647358
16	20.991522047618734	26.751313198320485	36.37474490813716	15.88241984592362
17	20.68758534077057	25.692655914945362	31.54224654721589	22.077512197068177
18	20.954184995268854	27.06054974194559	33.75674463778593	18.22852062499963
19	24.048277344626946	27.849510836975234	30.054659777275848	18.04755204112197
20	25.630784786729212	28.28254918576047	30.082826325539795	16.003839701970527
21	23.573554886360185	27.458811633677666	31.90227815568062	17.06535532428153
22	22.815142288707715	25.265215146576214	31.89054705629796	20.029095508418106
23	19.586708009363445	29.19691990160168	31.722738741270902	19.493633347763975
24	19.262762929964143	27.91733683797446	35.07807135962251	17.741828872438884
25	19.514177658865584	29.51705386241981	32.34782173731032	18.62094674140429
26	19.180764331741198	32.831119212384706	29.651098048766833	18.33701840710726
27	18.300991426769656	31.8919762257659	32.08074569298939	17.726286654475057
28	17.348985914939405	30.15148600872865	35.04162753819009	17.457900538141853
29	18.320761604409462	28.028633410290137	34.61013745632845	19.040467528971945
30	19.47350587775718	29.208829647167832	32.406000844400964	18.911663630674024
31	21.83020633038706	29.046142522734215	30.54772324371448	18.575927903164242
32	22.684730574758365	28.622334226762746	30.998566662121114	17.694368536357775
33	21.865101884895882	29.64526227343942	29.73476401136904	18.754871830295652
34	19.327790140755326	29.231934553566163	31.616742005732164	19.823533299946348
35	19.376798743760038	29.016904097369316	31.93711416146161	19.669182997409035
36	21.605112139186815	29.612391375676843	30.083779105185087	18.698717379951255
37	19.587660789008734	31.554156292782036	30.53176418465584	18.326418733553385
38	19.61207576741934	31.38747940358376	29.272904078313722	19.727540750683172
39	19.89046607002811	30.96992372403452	29.635079440980363	19.504530764957003
40	21.471663440118096	29.208234159889525	29.989870761395988	19.33023163859639
41	18.094416889924773	29.580771001198713	30.714757425279743	21.610054683596765
42	19.738795460243185	30.061448332248553	29.29154283012475	20.90821337738351
43	19.873077841501534	30.04036808259647	29.459767986246625	20.626786089655376
44	19.848484216907433	31.372413575442582	29.241164606379932	19.537937601270055
45	19.227867375455325	32.27725649483087	27.936392430880304	20.5584836988335
46	19.530553559019044	32.02083967279165	28.672533804324075	19.77607296386524
47	19.265680817627853	30.678373152575155	29.90846765045135	20.147478379345642
48	20.075603064853926	29.243606104220994	31.164171674218437	19.516619156706646
49	19.505602642057955	29.386225307375646	30.572376417036413	20.535795633529982
50	18.504052588672522	31.674027881905342	30.121890290996767	19.700029238425365
51	18.230962122840687	31.91001949029862	29.317267880547632	20.541750506313058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1091.0
1	1306.5
2	1522.0
3	14232.0
4	26942.0
5	20014.5
6	13087.0
7	12084.5
8	11082.0
9	11746.5
10	12411.0
11	12941.5
12	13472.0
13	13812.0
14	14152.0
15	13716.0
16	13280.0
17	12806.5
18	12333.0
19	12406.5
20	12480.0
21	12541.0
22	12602.0
23	13948.5
24	15295.0
25	17709.5
26	23973.5
27	27823.0
28	31321.5
29	34820.0
30	41288.5
31	47757.0
32	52921.5
33	58086.0
34	64191.0
35	70296.0
36	75995.5
37	81695.0
38	87612.0
39	93529.0
40	103000.0
41	112471.0
42	120116.0
43	127761.0
44	128861.5
45	129962.0
46	128145.5
47	126329.0
48	122885.5
49	119442.0
50	112344.5
51	105247.0
52	95467.5
53	85688.0
54	77232.0
55	68776.0
56	63745.0
57	58714.0
58	54346.5
59	49979.0
60	44442.0
61	38905.0
62	32477.0
63	26049.0
64	20700.5
65	15352.0
66	11997.5
67	8643.0
68	6641.5
69	4640.0
70	3746.0
71	2852.0
72	2185.5
73	1519.0
74	1508.0
75	1191.5
76	886.0
77	618.5
78	351.0
79	261.5
80	172.0
81	140.5
82	109.0
83	80.5
84	52.0
85	31.0
86	10.0
87	9.5
88	9.0
89	6.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1679297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.54484754722455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.92546593292393	15.992947399648887
2	8.159039927271284	3.8420670243864095
3	3.9715362613156517	2.8052764740285356
4	2.474326225131664	2.3303053501129853
5	1.6136234040364208	1.8996258523335525
6	1.410889500392209	1.9931506915628627
7	1.0847881361601368	1.7878819900849803
8	0.9187733072019363	1.7305901958823116
9	0.8590913553457178	1.820445749167809
>10	11.132850962631373	57.34516545733216
>50	0.4154108773897187	5.942902598457617
>100	0.03239025658072877	1.1828596381657013
>500	0.001036487782428377	0.1563682892609205
>1k	5.182438912141885E-4	0.16039494048652359
>5k	0.0	0.0
>10k+	2.5912194560709427E-4	1.0100183490887782
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16555	0.9858291892381157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009408698997258972	0.0
2	0.0	0.0	0.0	0.04942544409952498	0.0
3	0.0	0.0	0.0	0.09367014887777445	0.0
4	0.0	0.0	0.0	0.15405255889815797	0.0
5	0.0	0.0	0.0	0.3058422661387473	0.0
6	0.0	0.0	0.0	0.577146270135658	0.0
7	0.0	0.0	0.0	0.7294123671988934	0.0
8	0.0	0.0	0.0	0.9551615944052779	0.0
9	0.0	0.0	0.0	1.1045098038048065	0.0
10	0.0	0.0	0.0	1.2900636397254328	0.0
11	0.0	0.0	0.0	1.5141455025525563	0.0
12	0.0	0.0	0.0	1.6847526077876636	0.0
13	0.0	0.0	0.0	1.7878314556626969	0.0
14	0.0	0.0	0.0	1.855836102845417	0.0
15	0.0	0.0	0.0	1.9143129535752164	0.0
16	0.0	0.0	0.0	1.9990507932783779	0.0
17	0.0	0.0	0.0	2.10677444192421	0.0
18	0.0	0.0	0.0	2.2240258870229628	0.0
19	0.0	0.0	0.0	2.3125152965794613	0.0
20	0.0	0.0	0.0	2.395049833352885	0.0
21	0.0	0.0	0.0	2.5024161896317327	0.0
22	0.0	0.0	0.0	2.624074240589961	0.0
23	0.0	0.0	0.0	2.754783698178464	0.0
24	0.0	0.0	0.0	2.86834312215171	0.0
25	0.0	0.0	0.0	2.967610851445575	0.0
26	0.0	0.0	0.0	3.066461739644625	0.0
27	0.0	0.0	0.0	3.165133981660183	0.0
28	0.0	0.0	0.0	3.26928470663617	0.0
29	0.0	0.0	0.0	3.388560808481168	0.0
30	0.0	0.0	0.0	3.531239560363652	0.0
31	0.0	0.0	0.0	3.660281653572894	0.0
32	0.0	0.0	0.0	3.7870608951245672	0.0
33	0.0	0.0	0.0	3.915090659960686	0.0
34	0.0	0.0	0.0	4.047169738289296	0.0
35	0.0	0.0	0.0	4.190741721089242	0.0
36	0.0	0.0	0.0	4.332586790782095	0.0
37	0.0	0.0	0.0	4.475920578670718	0.0
38	0.0	0.0	0.0	4.636821241269412	0.0
39	0.0	0.0	0.0	4.826841231777345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	40	6.8175723E-9	45.000004	1
CGGTCTA	40	6.8175723E-9	45.000004	31
CTATGCG	55	1.8189894E-12	45.0	1
CGTTTTT	8980	0.0	42.544544	1
CGGGTAC	50	1.0822987E-9	40.5	6
TACGGGA	885	0.0	40.16949	4
CACGACC	180	0.0	40.0	27
CGAGGGA	1365	0.0	39.23077	4
CGACAGG	185	0.0	38.91892	2
CGTTAGG	295	0.0	38.898304	2
TAGGGAC	2370	0.0	38.73418	5
GGGCGAT	3085	0.0	38.727715	7
ACGGGAT	1305	0.0	38.62069	5
ACGTCGG	35	6.2500094E-6	38.57143	2
TAGGGCG	620	0.0	38.46774	5
TGGGCGA	860	0.0	38.4593	6
AGGGCGA	1505	0.0	38.421925	6
ACGGGAC	610	0.0	38.360657	5
GGCGATT	925	0.0	38.18919	8
TTAGGGA	3150	0.0	38.142857	4
>>END_MODULE