FastQCFastQC Report
Thu 26 May 2016
SRR1548016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548016_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2153031
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54850.25475713076123846No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25830.11997040451345103No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3250.042.92307331
TCGTTAG1050.042.8571431
AACGGGA9800.040.4081654
TACGCGG2150.039.767442
GCGATCG403.4600998E-739.3759
GGGCGAT31650.038.1753547
TAAGGGA33150.038.0090524
CGTTAGG4050.037.7777752
TAACGGG6850.037.773723
TAGGGAG35300.037.733715
TATGGGA30900.037.7184454
TAGGGAT33900.037.4999965
TCGATCA1700.037.05882317
CGTTTTT32650.037.0061261
AGGGATG44850.036.8227426
ATAGGGA35150.036.8065454
TTAGGGA32100.036.5887874
GGCGATA7200.036.5625048
TATAGCG800.036.56251
TACGGGA9300.036.532264
Produced by FastQC (version 0.11.5)