##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548016_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2153031
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89982958907698	31.0	31.0	31.0	30.0	33.0
2	31.248756288228083	31.0	31.0	33.0	30.0	34.0
3	31.476045166093755	31.0	31.0	34.0	30.0	34.0
4	35.340008573959224	37.0	35.0	37.0	33.0	37.0
5	35.16048584530367	37.0	35.0	37.0	32.0	37.0
6	35.187905329742115	37.0	35.0	37.0	32.0	37.0
7	35.5185930903921	35.0	35.0	37.0	35.0	37.0
8	35.594734585800204	37.0	35.0	37.0	35.0	37.0
9	37.42299576736238	39.0	37.0	39.0	35.0	39.0
10	36.576498898529564	39.0	35.0	39.0	32.0	39.0
11	36.24363095561559	38.0	35.0	39.0	31.0	39.0
12	35.63864431120592	37.0	35.0	39.0	30.0	39.0
13	35.31555374725213	37.0	35.0	39.0	30.0	39.0
14	36.283588113687166	38.0	35.0	40.0	30.0	41.0
15	36.535088440435835	38.0	35.0	40.0	30.0	41.0
16	36.69058736265293	38.0	35.0	40.0	31.0	41.0
17	36.646370628198106	38.0	35.0	40.0	31.0	41.0
18	36.56256830486881	38.0	35.0	40.0	31.0	41.0
19	36.512691642619174	38.0	35.0	40.0	31.0	41.0
20	36.382288039512666	38.0	35.0	40.0	30.0	41.0
21	36.261458845692424	38.0	34.0	40.0	30.0	41.0
22	36.16157314966668	38.0	34.0	40.0	30.0	41.0
23	36.09996279663414	38.0	34.0	40.0	30.0	41.0
24	35.9688587855911	38.0	34.0	40.0	30.0	41.0
25	35.78825061041852	38.0	34.0	40.0	29.0	41.0
26	35.55649547080372	37.0	34.0	40.0	29.0	41.0
27	35.646993006603246	38.0	34.0	40.0	29.0	41.0
28	35.689678875966024	38.0	34.0	40.0	29.0	41.0
29	35.68539282527748	38.0	34.0	40.0	29.0	41.0
30	35.62578337237132	38.0	34.0	40.0	29.0	41.0
31	35.550729645787726	38.0	34.0	40.0	29.0	41.0
32	35.43085631372702	38.0	34.0	40.0	28.0	41.0
33	35.32966780320395	37.0	34.0	40.0	27.0	41.0
34	35.20827800435758	37.0	34.0	40.0	27.0	41.0
35	35.09503393123462	37.0	34.0	40.0	26.0	41.0
36	35.00459631096812	37.0	33.0	40.0	26.0	41.0
37	34.944844268382575	37.0	33.0	40.0	26.0	41.0
38	34.88173509810124	37.0	33.0	40.0	26.0	41.0
39	34.86747845247003	37.0	33.0	40.0	26.0	41.0
40	34.65270356070117	37.0	33.0	40.0	24.0	41.0
41	34.60136105796897	37.0	33.0	40.0	24.0	41.0
42	34.55379137597183	37.0	33.0	40.0	24.0	41.0
43	34.51000241055517	37.0	33.0	40.0	24.0	41.0
44	34.318639164972545	36.0	33.0	40.0	24.0	41.0
45	34.1862941128112	36.0	33.0	40.0	23.0	41.0
46	34.14387252203986	36.0	33.0	40.0	23.0	41.0
47	34.0954459085819	36.0	33.0	40.0	23.0	41.0
48	34.016934266157804	36.0	33.0	40.0	23.0	41.0
49	33.89705257379016	36.0	33.0	40.0	24.0	41.0
50	33.762235657545105	35.0	32.0	40.0	23.0	41.0
51	33.42672214194779	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	6.0
13	14.0
14	41.0
15	85.0
16	227.0
17	577.0
18	1214.0
19	2322.0
20	4094.0
21	6296.0
22	9557.0
23	13499.0
24	18742.0
25	25724.0
26	33206.0
27	40146.0
28	47815.0
29	57883.0
30	69852.0
31	85273.0
32	104744.0
33	131961.0
34	196519.0
35	225860.0
36	184588.0
37	227916.0
38	305289.0
39	359522.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.14239321217391	24.95988213825068	29.68443092551849	12.213293724056923
2	28.66229051044783	26.9260869908515	30.476430669135745	13.935191829564925
3	29.3888940753756	26.683173628247804	30.482747345486434	13.445184950890162
4	26.032509517977214	29.473193836967514	29.907093766880273	14.587202878175
5	23.33579962387908	33.941731447433874	28.517378523579083	14.205090405107962
6	22.260478367473578	42.50101368721584	25.12286167732838	10.115646267982207
7	91.88910888881767	3.094103150395884	3.900036738904363	1.1167512218820816
8	92.4750270664937	2.5467817230685483	3.6647869909908404	1.31340421944691
9	87.04310342024802	5.604796215196158	5.106893491083036	2.245206873472793
10	40.0688146153028	38.09703622474549	11.332210265435101	10.501938894516615
11	36.965700911877256	23.4880036562409	23.021452083133035	16.52484334874881
12	35.344776735681	21.735079522775102	25.133219168697522	17.786924572846374
13	25.200148070325046	30.187814295288828	25.867857917512566	18.74417971687356
14	20.014807032504407	32.53079031374838	27.72254556483395	19.73185708891326
15	19.00307055495253	25.788899463128956	36.80411475728868	18.403915224629834
16	22.66321293097963	24.11762766072574	34.36100084021085	18.858158568083784
17	22.933157952672303	24.015864146870154	25.76827737268994	27.282700527767602
18	23.894825480915046	25.28765261624194	30.020840387342307	20.796681515500705
19	27.675913630597982	26.44676272659335	24.974233998488643	20.90308964432003
20	28.98137555845689	26.168132274918477	25.49703185880742	19.353460307817212
21	26.169990120903975	26.59381123634541	27.79068206635204	19.445516576398575
22	25.846214011781534	23.285730674569944	26.906347377255603	23.961707936392926
23	22.77524104390508	27.616276774463532	26.7490807145833	22.859401467048084
24	22.958796227272156	25.08830574199814	32.22252721860484	19.73037081212486
25	21.928063274518575	27.047775902901538	29.46687716061682	21.557283661963066
26	21.300900915964515	30.719251139440168	25.648399860475767	22.33144808411955
27	20.618281854743383	30.609731118595136	28.19095498392731	20.581032042734172
28	18.982448464513517	28.415522117424224	32.11644421283298	20.485585205229278
29	20.148107481963798	26.264090020069382	31.498989099553143	22.088813398413677
30	21.619475056327566	28.180690384857442	29.62474762323441	20.575086935580583
31	26.35094431989135	26.723395993833808	25.915279436292373	21.010380249982465
32	27.251256484463067	25.98155809182497	26.969235463864667	19.7979499598473
33	26.179836704627107	27.05163093332144	25.74384669798066	21.02468566407079
34	21.588959936015783	27.38395313397717	27.967595450320964	23.05949147968608
35	21.726068969745445	27.0628709015337	28.851697908669223	22.35936222005164
36	27.366396489414225	26.599431220451542	25.76107821949614	20.27309407063809
37	22.3600589122962	29.93830557943662	27.471364787594794	20.23027072067239
38	22.2528147527834	29.793579377166424	25.737808698527797	22.215797171522382
39	22.065358092846783	28.800189128721325	27.197146720135475	21.93730605829642
40	24.393796466469826	26.303011893465538	26.499200429533992	22.80399121053064
41	20.644523929288525	25.153376797640163	28.260670654533076	25.941428618538236
42	22.568695016467483	25.9770063691605	26.681037105364485	24.773261509007536
43	22.285466395978506	26.096001404531567	27.481629386664657	24.136902812825266
44	21.230349214665278	27.960396297127165	28.072517302351898	22.73673718585566
45	20.62854645381325	30.656502391280014	25.467399215338748	23.247551939567987
46	22.323923807878288	28.983233404442387	26.85609264334791	21.83675014433141
47	21.78064319556941	27.016889213392652	27.978185172438298	23.22428241859964
48	22.668136222841195	26.13371567803715	29.296884252943872	21.901263846177784
49	22.581421261468133	25.512405534337407	29.158706957772555	22.747466246421904
50	21.164720805227606	28.765911870288907	28.314176618915383	21.755190705568104
51	20.58363302711387	29.530647724069	26.6728625830283	23.212856665788834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1485.0
1	1912.0
2	2339.0
3	6317.5
4	10296.0
5	7990.5
6	5685.0
7	5534.0
8	5383.0
9	5797.0
10	6211.0
11	6374.5
12	6538.0
13	6664.5
14	6791.0
15	6604.0
16	6417.0
17	6490.0
18	6563.0
19	6963.0
20	7363.0
21	8047.0
22	8731.0
23	9750.5
24	10770.0
25	12606.0
26	18225.0
27	22008.0
28	25261.5
29	28515.0
30	33937.0
31	39359.0
32	44680.0
33	50001.0
34	57393.5
35	64786.0
36	67794.5
37	70803.0
38	80673.0
39	90543.0
40	108915.0
41	127287.0
42	144819.0
43	162351.0
44	166087.0
45	169823.0
46	169510.5
47	169198.0
48	165843.5
49	162489.0
50	155598.0
51	148707.0
52	141406.0
53	134105.0
54	127667.0
55	121229.0
56	115071.5
57	108914.0
58	104431.5
59	99949.0
60	90563.0
61	81177.0
62	73124.5
63	65072.0
64	56464.5
65	47857.0
66	39818.0
67	31779.0
68	26692.0
69	21605.0
70	18184.0
71	14763.0
72	12169.5
73	9576.0
74	7635.5
75	4523.5
76	3352.0
77	2394.5
78	1437.0
79	1104.5
80	772.0
81	583.0
82	394.0
83	320.5
84	247.0
85	186.5
86	126.0
87	80.5
88	35.0
89	32.0
90	29.0
91	23.0
92	17.0
93	12.5
94	8.0
95	7.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2153031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.598674152943623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86494776444293	15.456484133242368
2	6.454158496068427	2.5298589859179557
3	2.0971413501360456	1.2330356992194182
4	1.1148741225522574	0.8740021859780251
5	0.7612901485117243	0.7460138778263671
6	0.5462120811881436	0.6423019558564569
7	0.4367996951698006	0.5992486427016618
8	0.3522176105528914	0.5522398544123613
9	0.31248834248089447	0.5511921480788852
>10	6.082910056927118	32.291727192892246
>50	2.680601807348622	36.19834791820788
>100	0.29320577457179825	7.4782677404191205
>500	0.0021826731111013894	0.3130837627613317
>1k	7.27557703700463E-4	0.27349081082870846
>5k	2.42519234566821E-4	0.260705091657189
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5485	0.25475713076123846	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2583	0.11997040451345103	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01574524472708475	0.0
2	0.0	0.0	0.0	0.0696692244561272	0.0
3	0.0	0.0	0.0	0.10636168266968753	0.0
4	0.0	0.0	0.0	0.20022935108690956	0.0
5	0.0	0.0	0.0	0.35763535220811965	0.0
6	0.0	0.0	0.0	0.570172932948945	0.0
7	0.0	0.0	0.0	0.6706824007643178	0.0
8	0.0	0.0	0.0	0.9043529795901685	0.0
9	0.0	0.0	0.0	0.9845654800139896	0.0
10	0.0	0.0	0.0	1.1373268661714577	0.0
11	0.0	0.0	0.0	1.3659812608364672	0.0
12	0.0	0.0	0.0	1.5484681827618831	0.0
13	0.0	0.0	0.0	1.635647605631317	0.0
14	0.0	0.0	0.0	1.6800036785350514	0.0
15	0.0	0.0	0.0	1.739408303921309	0.0
16	0.0	0.0	0.0	1.8579853239456376	0.0
17	0.0	0.0	0.0	2.001550372474897	0.0
18	0.0	0.0	0.0	2.164762142300784	0.0
19	0.0	0.0	0.0	2.26647921000673	0.0
20	0.0	0.0	0.0	2.3884932451042276	0.0
21	0.0	0.0	0.0	2.5403721544185847	0.0
22	0.0	0.0	0.0	2.706928047018366	0.0
23	0.0	0.0	0.0	2.88207647730107	0.0
24	0.0	0.0	0.0	3.0147731268151734	0.0
25	0.0	0.0	0.0	3.136322700416297	0.0
26	0.0	0.0	0.0	3.2553641819369994	0.0
27	0.0	0.0	0.0	3.375288140300813	0.0
28	0.0	0.0	0.0	3.4996244828801815	0.0
29	0.0	0.0	0.0	3.6491346385630306	0.0
30	0.0	0.0	0.0	3.836173283152913	0.0
31	0.0	0.0	0.0	3.9990599299313385	0.0
32	0.0	0.0	0.0	4.144250593697908	0.0
33	0.0	0.0	0.0	4.293017610986558	0.0
34	0.0	0.0	0.0	4.457344088403743	0.0
35	0.0	0.0	0.0	4.645450994435286	0.0
36	0.0	0.0	0.0	4.810288379498484	0.0
37	0.0	0.0	0.0	4.9783769950363	0.0
38	0.0	0.0	0.0	5.171128516031585	0.0
39	0.0	0.0	0.0	5.454635813418386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	325	0.0	42.923073	31
TCGTTAG	105	0.0	42.857143	1
AACGGGA	980	0.0	40.408165	4
TACGCGG	215	0.0	39.76744	2
GCGATCG	40	3.4600998E-7	39.375	9
GGGCGAT	3165	0.0	38.175354	7
TAAGGGA	3315	0.0	38.009052	4
CGTTAGG	405	0.0	37.777775	2
TAACGGG	685	0.0	37.77372	3
TAGGGAG	3530	0.0	37.73371	5
TATGGGA	3090	0.0	37.718445	4
TAGGGAT	3390	0.0	37.499996	5
TCGATCA	170	0.0	37.058823	17
CGTTTTT	3265	0.0	37.006126	1
AGGGATG	4485	0.0	36.822742	6
ATAGGGA	3515	0.0	36.806545	4
TTAGGGA	3210	0.0	36.588787	4
GGCGATA	720	0.0	36.562504	8
TATAGCG	80	0.0	36.5625	1
TACGGGA	930	0.0	36.53226	4
>>END_MODULE