FastQCFastQC Report
Thu 26 May 2016
SRR1548015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548015_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2038193
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133150.6532747389476855No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTA253.8918704E-545.00000413
ATCGGTC207.034572E-445.031
CTACGCG302.1663327E-644.9999961
CGACGGT2400.044.06249628
CGTTTTT69500.041.9568331
CGGTCTA2550.040.58823431
CCGCTCG451.929584E-840.019
TACGGGA10500.039.6428534
GCGCGAC2750.039.2727289
GGCGATA6800.038.7132348
AGGGCGA16300.038.512276
GGGCGAT30500.038.3606577
CGTAAGG3350.038.283582
ACGCGAG659.094947E-1238.0769271
TAATGCG1300.038.0769271
GACCGAT9850.037.9187779
TAGGGAC28050.037.7807465
TTAGGGA34900.037.650434
GCGGGAT15350.037.524435
CACGCCG3000.037.526
Produced by FastQC (version 0.11.5)