##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548015_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2038193
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.906395517990692	31.0	31.0	33.0	30.0	33.0
2	31.252343620059534	31.0	31.0	33.0	30.0	34.0
3	31.366176804650003	31.0	31.0	34.0	30.0	34.0
4	35.271773085277005	37.0	35.0	37.0	33.0	37.0
5	35.122577695046544	37.0	35.0	37.0	32.0	37.0
6	35.17358709405831	37.0	35.0	37.0	32.0	37.0
7	35.5757212393527	36.0	35.0	37.0	35.0	37.0
8	35.67528295897395	37.0	35.0	37.0	35.0	37.0
9	37.500004170360704	39.0	37.0	39.0	35.0	39.0
10	36.643322786409335	39.0	37.0	39.0	32.0	39.0
11	36.220530146065656	38.0	35.0	39.0	31.0	39.0
12	35.90976369754974	37.0	35.0	39.0	31.0	39.0
13	35.77509440960694	37.0	35.0	39.0	30.0	39.0
14	36.81727638157918	39.0	35.0	40.0	31.0	41.0
15	36.97794222627592	39.0	35.0	41.0	31.0	41.0
16	37.061225801481996	39.0	35.0	41.0	31.0	41.0
17	36.988946581604395	39.0	35.0	41.0	31.0	41.0
18	36.89174528614317	38.0	35.0	40.0	31.0	41.0
19	36.832299002106275	38.0	35.0	40.0	31.0	41.0
20	36.691321675621495	38.0	35.0	40.0	31.0	41.0
21	36.57041408738034	38.0	35.0	40.0	30.0	41.0
22	36.45472239380667	38.0	35.0	40.0	30.0	41.0
23	36.3754266646976	38.0	35.0	40.0	30.0	41.0
24	36.28834855187904	38.0	35.0	40.0	30.0	41.0
25	36.13323566512101	38.0	34.0	40.0	30.0	41.0
26	35.9141484638599	38.0	34.0	40.0	29.0	41.0
27	35.987968264045655	38.0	34.0	40.0	30.0	41.0
28	36.02402373082431	38.0	34.0	40.0	30.0	41.0
29	36.0055431453253	38.0	34.0	40.0	30.0	41.0
30	35.937179648836	38.0	34.0	40.0	29.0	41.0
31	35.85778235917796	38.0	34.0	40.0	29.0	41.0
32	35.71565499439945	38.0	34.0	40.0	29.0	41.0
33	35.57033362395023	38.0	34.0	40.0	28.0	41.0
34	35.406683272879455	38.0	34.0	40.0	27.0	41.0
35	35.250465485849475	38.0	34.0	40.0	26.0	41.0
36	35.191072189925094	38.0	34.0	40.0	25.0	41.0
37	35.14391865735973	38.0	34.0	40.0	25.0	41.0
38	35.07061941631632	38.0	34.0	40.0	25.0	41.0
39	35.05003745965176	38.0	34.0	40.0	25.0	41.0
40	34.897420411119064	38.0	34.0	40.0	24.0	41.0
41	34.815020952382824	38.0	34.0	40.0	24.0	41.0
42	34.79838023190149	38.0	33.0	40.0	24.0	41.0
43	34.75785708222921	38.0	33.0	40.0	24.0	41.0
44	34.620900474096416	37.0	33.0	40.0	24.0	41.0
45	34.50667772875287	37.0	33.0	40.0	24.0	41.0
46	34.458546859890106	37.0	33.0	40.0	23.0	41.0
47	34.424832682675294	37.0	33.0	40.0	23.0	41.0
48	34.34447768194671	37.0	33.0	40.0	23.0	41.0
49	34.20548740968103	37.0	33.0	40.0	23.0	41.0
50	34.10470156653467	37.0	33.0	40.0	23.0	41.0
51	33.775163588531605	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	6.0
13	7.0
14	25.0
15	55.0
16	178.0
17	428.0
18	984.0
19	1925.0
20	3298.0
21	5235.0
22	8200.0
23	11732.0
24	17809.0
25	25067.0
26	33336.0
27	38662.0
28	43350.0
29	51116.0
30	60815.0
31	74487.0
32	90368.0
33	113576.0
34	161097.0
35	192271.0
36	178495.0
37	221286.0
38	309677.0
39	394662.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.267689566199078	26.47452915401044	29.91782426884991	14.339957010940573
2	30.453004205195484	26.558083557347125	29.863658642729124	13.125253594728273
3	27.807719877361958	26.579033487015213	32.3060671879454	13.307179447677427
4	24.800399177114237	29.494311873311315	31.84428560003886	13.861003349535594
5	22.410978744407423	33.4299058038174	30.281528785546808	13.87758666622837
6	21.601634388892514	41.435477405721635	27.04680076911264	9.916087436273209
7	90.94766786069817	2.516346587393834	5.566940912857614	0.9690446390503746
8	91.78458565994487	1.92067188926662	5.257107643878671	1.0376348069098462
9	86.75856506228801	5.282276997320666	6.32849784097973	1.6306600994115867
10	37.76178212760028	41.7587539550965	11.568678726695657	8.910785190607564
11	27.063236896604003	25.757374301648568	29.724172342854676	17.455216458892757
12	28.40923308047864	24.237106103298363	29.006575922888562	18.34708489333444
13	22.948562771042784	28.63561007225518	29.55254973400458	18.863277422697458
14	17.913367379831055	32.20465382817034	29.91944335006547	19.962535441933124
15	17.548240034187145	29.10813647186503	35.73434900424052	17.609274489707303
16	21.511505534559287	26.981203448348612	34.401452659291834	17.10583835780027
17	20.908176997958485	26.735888112656653	29.612308549779144	22.74362633960572
18	21.766584420611785	27.6788311999894	31.553586927243888	19.000997452154923
19	24.048851114688354	29.011972860273783	27.820034707213694	19.11914131782417
20	25.976489959488624	27.879057576981182	28.675743661174387	17.468708802355813
21	24.29912182016129	27.257330390203478	30.781677691955572	17.66187009767966
22	23.07401703371565	25.011419428876458	30.450551051838566	21.464012485569324
23	20.282377576608297	29.38087806208735	30.137479620428486	20.199264740875865
24	19.78163991339387	28.150670716659317	33.30454966727881	18.763139702668
25	20.468375664129944	29.83814584781716	30.619180813593218	19.074297674459682
26	19.895122787684976	32.27658028459523	28.036648148629695	19.791648779090107
27	18.621151186369495	31.76902285504857	30.831280452832484	18.778545505749456
28	17.44589447613646	30.370234810933017	33.64681362363623	18.537057089294294
29	18.8442900157149	28.059020907244797	33.15647733065514	19.940211746385156
30	19.702108681562542	29.20454539879197	31.811020840519028	19.282325079126462
31	22.48516210192067	29.075362343016586	29.090817209165177	19.34865834589757
32	22.684946911308206	29.496814089735366	29.41222936198878	18.406009636967646
33	22.23665766686472	29.15386325043801	28.803749203338448	19.805729879358825
34	19.00570750660021	29.775394184947157	30.296787399426844	20.92211090902579
35	19.69082417612071	28.764645938829148	30.896043701455163	20.648486183594976
36	22.553605080578727	29.305958758567023	28.3664991490011	19.773937011853146
37	20.059974693269968	30.760433383884646	29.957172848694896	19.222419074150483
38	19.877361957380877	31.4935337330665	28.469678779193135	20.15942553035949
39	20.008262220506104	30.30429404869902	28.58674325738534	21.100700473409535
40	22.25878511014413	28.697086095379582	29.080857406536083	19.9632713879402
41	18.892617136846216	28.433273983376452	30.180655119510273	22.49345376026706
42	20.757209940373656	29.25939790785269	28.28942107052669	21.693971081246968
43	20.767660373674133	29.495293134654077	28.32003642442104	21.417010067250747
44	19.982258794922757	30.30424498563188	29.321266435514204	20.39222978393116
45	19.68478941886269	31.23830765781258	27.552788180510873	21.524114742813854
46	20.441685355606655	31.163339291225117	28.113824353238382	20.28115099992984
47	20.013806347092743	29.717058198119613	29.733249010275276	20.535886444512368
48	20.497813504412978	29.17623600905312	30.18281389446436	20.143136592069546
49	20.255883520353567	28.85065349552275	29.827646351449545	21.06581663267414
50	19.234243273330836	30.71274408262613	29.500935387374994	20.55207725666804
51	19.00050682148354	30.489359937945032	29.071976991384034	21.438156249187394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1505.0
1	1757.0
2	2009.0
3	11780.0
4	21551.0
5	16043.0
6	10535.0
7	9926.5
8	9318.0
9	9803.0
10	10288.0
11	10934.5
12	11581.0
13	12019.5
14	12458.0
15	12382.0
16	12306.0
17	12039.0
18	11772.0
19	11821.0
20	11870.0
21	12334.0
22	12798.0
23	14588.5
24	16379.0
25	18626.0
26	23944.5
27	27016.0
28	34353.5
29	41691.0
30	48631.5
31	55572.0
32	63456.5
33	71341.0
34	78219.0
35	85097.0
36	89902.5
37	94708.0
38	102441.0
39	110174.0
40	121986.0
41	133798.0
42	144047.0
43	154296.0
44	156659.0
45	159022.0
46	159293.0
47	159564.0
48	158813.5
49	158063.0
50	149123.5
51	140184.0
52	127761.0
53	115338.0
54	103369.0
55	91400.0
56	83021.0
57	74642.0
58	68965.0
59	63288.0
60	57828.5
61	52369.0
62	43265.0
63	34161.0
64	27355.5
65	20550.0
66	16399.5
67	12249.0
68	9661.5
69	7074.0
70	5679.0
71	4284.0
72	3676.0
73	3068.0
74	2427.0
75	1534.0
76	1282.0
77	846.0
78	410.0
79	325.5
80	241.0
81	182.5
82	124.0
83	105.0
84	86.0
85	56.5
86	27.0
87	22.0
88	17.0
89	15.5
90	14.0
91	10.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2038193.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.767820072493116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.61108773834383	16.987318210396097
2	7.21721652563623	3.2864057455981923
3	3.021386190198971	2.063711314438969
4	1.7687723800349588	1.6108436519132543
5	1.187075402411805	1.351355958729717
6	0.9213162988487025	1.2585818233225532
7	0.7481128254866579	1.192302874322327
8	0.6120759720429513	1.1148508481736195
9	0.5649018259892363	1.1575424819471174
>10	7.699939669253758	41.77497430224504
>50	1.522038524595392	23.11144548854193
>100	0.12364785503846726	3.8327314731055417
>500	8.831971342297471E-4	0.13955280567834996
>1k	0.0013247957013446205	0.44902221193048336
>5k	0.0	0.0
>10k+	2.2079928355743677E-4	0.669360809656783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13315	0.6532747389476855	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02418809209922711	0.0
2	0.0	0.0	0.0	0.07668557393730623	0.0
3	0.0	0.0	0.0	0.134776245429162	0.0
4	0.0	0.0	0.0	0.1980676020376873	0.0
5	0.0	0.0	0.0	0.36453858883825035	0.0
6	0.0	0.0	0.0	0.6227575111876059	0.0
7	0.0	0.0	0.0	0.7591037747651964	0.0
8	0.0	0.0	0.0	1.0106010569165922	0.0
9	0.0	0.0	0.0	1.131541517412728	0.0
10	0.0	0.0	0.0	1.3055682165526032	0.0
11	0.0	0.0	0.0	1.5531404533329278	0.0
12	0.0	0.0	0.0	1.743063586225642	0.0
13	0.0	0.0	0.0	1.8546330008983447	0.0
14	0.0	0.0	0.0	1.9236647363620618	0.0
15	0.0	0.0	0.0	1.9968668325325423	0.0
16	0.0	0.0	0.0	2.1168750947530484	0.0
17	0.0	0.0	0.0	2.273778783461625	0.0
18	0.0	0.0	0.0	2.445597644580273	0.0
19	0.0	0.0	0.0	2.5671268618820693	0.0
20	0.0	0.0	0.0	2.691501737077892	0.0
21	0.0	0.0	0.0	2.832901496570737	0.0
22	0.0	0.0	0.0	3.0068300695763357	0.0
23	0.0	0.0	0.0	3.1880690395855544	0.0
24	0.0	0.0	0.0	3.3351110517993146	0.0
25	0.0	0.0	0.0	3.4616447019492265	0.0
26	0.0	0.0	0.0	3.5855289464736657	0.0
27	0.0	0.0	0.0	3.7200598765671358	0.0
28	0.0	0.0	0.0	3.857093023084664	0.0
29	0.0	0.0	0.0	4.0066372517224815	0.0
30	0.0	0.0	0.0	4.18331335648783	0.0
31	0.0	0.0	0.0	4.349980595556946	0.0
32	0.0	0.0	0.0	4.5089939961524745	0.0
33	0.0	0.0	0.0	4.669430225695015	0.0
34	0.0	0.0	0.0	4.833202743802967	0.0
35	0.0	0.0	0.0	5.023714633501342	0.0
36	0.0	0.0	0.0	5.193865350337284	0.0
37	0.0	0.0	0.0	5.376821527696347	0.0
38	0.0	0.0	0.0	5.564389633366418	0.0
39	0.0	0.0	0.0	5.806417743560105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGGTA	25	3.8918704E-5	45.000004	13
ATCGGTC	20	7.034572E-4	45.0	31
CTACGCG	30	2.1663327E-6	44.999996	1
CGACGGT	240	0.0	44.062496	28
CGTTTTT	6950	0.0	41.956833	1
CGGTCTA	255	0.0	40.588234	31
CCGCTCG	45	1.929584E-8	40.0	19
TACGGGA	1050	0.0	39.642853	4
GCGCGAC	275	0.0	39.272728	9
GGCGATA	680	0.0	38.713234	8
AGGGCGA	1630	0.0	38.51227	6
GGGCGAT	3050	0.0	38.360657	7
CGTAAGG	335	0.0	38.28358	2
ACGCGAG	65	9.094947E-12	38.076927	1
TAATGCG	130	0.0	38.076927	1
GACCGAT	985	0.0	37.918777	9
TAGGGAC	2805	0.0	37.780746	5
TTAGGGA	3490	0.0	37.65043	4
GCGGGAT	1535	0.0	37.52443	5
CACGCCG	300	0.0	37.5	26
>>END_MODULE