FastQCFastQC Report
Thu 26 May 2016
SRR1548014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548014_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1186787
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.3710859657208918No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27340.23036989788395054No Hit
TTTCTAGGGTTTGCATATGTTTTTACTTATAAATGTAATGGATTTATAAAT14900.12554906651319908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12460.10498935360768191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG406.8157533E-945.01
GGTACGA207.03338E-445.08
GATCGAC253.890883E-545.011
TAGTTCG207.03338E-445.011
TAGCACG253.890883E-545.01
TACGACG406.8157533E-945.01
CCGAGTC253.890883E-545.028
TTCTCGG253.890883E-545.02
CGGACGG453.8562575E-1045.02
CGACCCA302.1655615E-644.99999633
CGTGTTG302.1655615E-644.99999620
GCTCGAC302.1655615E-644.99999643
CACGACG2700.043.33333626
CGGTCTA2450.043.16326531
TACGCGG850.042.3529432
CGACGGT2550.042.3529428
TACTCGC650.041.5384645
TCATGCA3850.041.49350724
AAGGGTC3550.041.197185
TCACGAC2900.041.1206925
Produced by FastQC (version 0.11.5)