FastQCFastQC Report
Thu 26 May 2016
SRR1548013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548013_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2460510
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121490.49375942385928123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70850.040.2364161
AGGGCGA21800.039.5298166
GGGCGAT43700.038.564077
TAGGGAC29700.038.5606085
CGTTAGG4300.038.1976742
TATGGGA33750.038.0666664
ACGGGAT13000.037.5576935
ACGCGAA301.1403004E-437.50000414
TAGGGAT42950.037.24685
TACGGGA12600.037.1428574
AGGGATG48000.036.93756
ATAGGGA40300.036.79284
TAAGGGA43200.036.6666644
CGGTAGT4850.036.64948712
TTAGGGA38550.036.1284074
TTTAGCG1750.036.01
TGGGCGA13700.035.9671556
GTAGGGA40400.035.922034
CGAGGGA19000.035.8815774
AGGGACC31650.035.8293846
Produced by FastQC (version 0.11.5)