##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548013_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2460510
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.82981129928348	31.0	31.0	31.0	30.0	33.0
2	31.17214398640932	31.0	31.0	33.0	30.0	34.0
3	31.312207225331335	31.0	31.0	34.0	30.0	34.0
4	35.21655469800976	37.0	35.0	37.0	33.0	37.0
5	35.051909563464484	35.0	35.0	37.0	32.0	37.0
6	35.085521294365805	37.0	35.0	37.0	32.0	37.0
7	35.48511731307737	35.0	35.0	37.0	35.0	37.0
8	35.564563444164015	37.0	35.0	37.0	35.0	37.0
9	37.367090562525654	39.0	37.0	39.0	35.0	39.0
10	36.49327009441132	38.0	35.0	39.0	32.0	39.0
11	36.132687125839766	38.0	35.0	39.0	31.0	39.0
12	35.717684951493794	37.0	35.0	39.0	30.0	39.0
13	35.54423351256447	37.0	35.0	39.0	30.0	39.0
14	36.51536226229521	38.0	35.0	40.0	30.0	41.0
15	36.683089278239066	38.0	35.0	40.0	31.0	41.0
16	36.77067721732487	38.0	35.0	40.0	31.0	41.0
17	36.688889701728506	38.0	35.0	40.0	31.0	41.0
18	36.595858582163864	38.0	35.0	40.0	30.0	41.0
19	36.535664557347864	38.0	35.0	40.0	30.0	41.0
20	36.41013570357365	38.0	35.0	40.0	30.0	41.0
21	36.29188460928832	38.0	34.0	40.0	30.0	41.0
22	36.17924048266416	38.0	34.0	40.0	30.0	41.0
23	36.0744552958533	38.0	34.0	40.0	30.0	41.0
24	35.97408301530984	38.0	34.0	40.0	29.0	41.0
25	35.808931888104496	38.0	34.0	40.0	29.0	41.0
26	35.54832656644354	38.0	34.0	40.0	28.0	41.0
27	35.641166668698766	38.0	34.0	40.0	29.0	41.0
28	35.657946929701566	38.0	34.0	40.0	29.0	41.0
29	35.64378563793685	38.0	34.0	40.0	29.0	41.0
30	35.55971729438206	38.0	34.0	40.0	28.0	41.0
31	35.48131769429915	38.0	34.0	40.0	27.0	41.0
32	35.35759212520981	38.0	34.0	40.0	27.0	41.0
33	35.20506602289769	38.0	34.0	40.0	27.0	41.0
34	35.0277117345591	38.0	34.0	40.0	25.0	41.0
35	34.88844020142166	38.0	33.0	40.0	25.0	41.0
36	34.818823739793785	38.0	33.0	40.0	24.0	41.0
37	34.766114748568384	38.0	33.0	40.0	24.0	41.0
38	34.69230362810962	37.0	33.0	40.0	24.0	41.0
39	34.65233102080463	37.0	33.0	40.0	24.0	41.0
40	34.473799740704166	37.0	33.0	40.0	23.0	41.0
41	34.38457067843658	37.0	33.0	40.0	23.0	41.0
42	34.34535888901082	37.0	33.0	40.0	23.0	41.0
43	34.311105014814004	37.0	33.0	40.0	23.0	41.0
44	34.1609259868889	37.0	33.0	40.0	23.0	41.0
45	34.04077406716494	37.0	33.0	40.0	23.0	41.0
46	33.973604252776866	36.0	33.0	40.0	23.0	41.0
47	33.91856403753693	36.0	33.0	40.0	23.0	41.0
48	33.811216780261006	36.0	32.0	40.0	23.0	41.0
49	33.66427935671873	36.0	32.0	40.0	22.0	41.0
50	33.55491056732141	36.0	32.0	40.0	22.0	41.0
51	33.2225786523932	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	14.0
14	29.0
15	109.0
16	333.0
17	742.0
18	1658.0
19	3121.0
20	5150.0
21	8261.0
22	12314.0
23	17483.0
24	24434.0
25	34064.0
26	44381.0
27	51642.0
28	59453.0
29	68324.0
30	81480.0
31	96579.0
32	116689.0
33	144462.0
34	201760.0
35	227760.0
36	216389.0
37	266545.0
38	358326.0
39	418967.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72728418092184	25.24399413129798	29.101324522151913	13.927397165628264
2	30.92911632141304	26.377620899732168	29.070802394625506	13.622460384229285
3	28.398990453198724	26.499790693799252	31.58780090306481	13.513417949937208
4	25.6904869315711	28.635892558859748	30.893148168469136	14.780472341100015
5	23.35584899065641	33.00921353703094	29.501607390337774	14.133330081974876
6	21.84839728349001	41.724276674348	26.20871282782838	10.218613214333613
7	90.66250492784016	2.7108607565098293	5.421111883308745	1.2055224323412626
8	91.23108623821891	2.157642114846109	5.259681935858826	1.351589711076159
9	85.66658944690329	5.5131253276759695	6.635006563679887	2.1852786617408584
10	38.24556697595214	39.37200011379755	12.065303534633063	10.317129375617249
11	31.85888291451772	24.901869937533274	26.333321140739113	16.90592600720989
12	30.742650913834936	22.70208208867267	27.5455088579197	19.009758139572693
13	25.355922146221722	25.99977240490793	28.924125486179697	19.72017996269066
14	20.51485261185689	28.896326371362036	29.960536636713524	20.628284380067548
15	19.885592824251884	26.472154146904504	34.96584041519847	18.676412613645137
16	24.443469036906983	24.890855960756102	32.602184100044305	18.063490902292614
17	23.930526598144287	24.63306387700111	28.556884548325346	22.879524976529257
18	24.171818037723884	25.717473206774205	29.89522497368432	20.215483781817593
19	26.516169412032465	26.588877915554093	27.09141600725053	19.803536665162913
20	28.055809567935103	26.97538315227331	26.618180783658673	18.350626496132914
21	26.531247586882394	25.95978069587199	28.800736432690783	18.708235284554828
22	25.641960406582378	23.78145181283555	28.802524679842794	21.774063100739276
23	23.25700769352695	26.812368167575013	28.69283197385908	21.237792165038954
24	22.61726227489423	25.546980097622036	31.58832924881427	20.24742837866946
25	23.272898707991434	26.95274556900805	28.666292760443973	21.10806296255654
26	22.219661777436386	29.541721025315894	26.779570089127862	21.45904710811986
27	21.072541871400645	29.08474259401506	29.003946336328646	20.838769198255648
28	19.995610666081422	28.210655514507156	30.993127441058967	20.800606378352455
29	21.3806893692771	26.396763272654855	30.75695689105104	21.465590467017
30	22.62949551109323	26.495157507996307	30.110546187578997	20.764800793331464
31	24.659115386647485	26.50385489187201	27.955830295345272	20.881199426135232
32	25.66943438555421	26.221271199873197	27.774282567435204	20.335011847137384
33	24.820789185981766	26.45797009563058	27.719131399587894	21.00210931879976
34	21.696396275568887	27.059186916533562	29.552247298324332	21.69216950957322
35	22.08123519107827	26.988347944125408	29.43662086315439	21.493796001641936
36	24.285859435645456	27.416633137032566	27.54116016598185	20.756347261340128
37	22.742886637323156	28.789763097894337	27.67320596136573	20.79414430341677
38	22.44209533795839	28.626057199523675	27.438701732567637	21.493145729950296
39	22.518055199938225	27.777452641931955	27.275808673811525	22.42868348431829
40	24.049770169598986	26.110765654274925	28.009477709905674	21.82998646622042
41	20.824869640846817	26.275568886125235	28.74469114126746	24.15487033176049
42	22.284119958870317	26.712145043100822	27.480441046774857	23.52329395125401
43	22.13630507496413	26.44378604435666	28.033944182303667	23.38596469837554
44	22.052663878626788	27.39330464009494	28.164201730535538	22.38982975074273
45	21.40800078032603	28.166152545610462	26.84203681350614	23.583809860557363
46	21.754311098105678	28.338190050030278	27.50884979130343	22.398649060560615
47	21.658314739627148	27.140755371853803	28.603297690316236	22.59763219820281
48	21.878838127054962	26.309626865974938	29.705752059532376	22.105782947437728
49	22.353130042145732	26.082194341823445	29.070111480953138	22.494564135077688
50	20.802760403331018	27.748352983731017	28.79776956809767	22.651117044840298
51	20.51086969774559	28.19000938829755	27.96387740752933	23.33524350642753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1481.0
1	2094.0
2	2707.0
3	12380.0
4	22053.0
5	16765.5
6	11478.0
7	10731.0
8	9984.0
9	10098.5
10	10213.0
11	10331.5
12	10450.0
13	10364.5
14	10279.0
15	10076.0
16	9873.0
17	9864.0
18	9855.0
19	9775.5
20	9696.0
21	10320.5
22	10945.0
23	12221.5
24	13498.0
25	15748.0
26	22691.5
27	27385.0
28	31430.0
29	35475.0
30	41754.0
31	48033.0
32	53543.5
33	59054.0
34	67292.5
35	75531.0
36	82951.0
37	90371.0
38	99243.5
39	108116.0
40	120029.0
41	131942.0
42	146424.0
43	160906.0
44	167126.5
45	173347.0
46	175967.0
47	178587.0
48	181158.0
49	183729.0
50	178786.5
51	173844.0
52	165272.0
53	156700.0
54	147635.5
55	138571.0
56	129568.5
57	120566.0
58	116801.5
59	113037.0
60	105910.0
61	98783.0
62	88379.5
63	77976.0
64	66542.5
65	55109.0
66	45791.0
67	36473.0
68	30564.0
69	24655.0
70	20527.0
71	16399.0
72	13558.5
73	10718.0
74	8784.5
75	5391.5
76	3932.0
77	2953.5
78	1975.0
79	1496.5
80	1018.0
81	718.5
82	419.0
83	332.5
84	246.0
85	195.0
86	144.0
87	94.5
88	45.0
89	38.0
90	31.0
91	23.5
92	16.0
93	14.5
94	13.0
95	7.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2460510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.197368742291548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00100770292299	17.398260317281537
2	6.264764549147249	2.9065210666040917
3	2.4485580608936717	1.704003126763826
4	1.4567417987419553	1.3517030667090482
5	1.0720931996874226	1.2434870639626172
6	0.8658660059802691	1.2051487813283714
7	0.7111393750421988	1.1547593617011656
8	0.6292934086196548	1.1678360997474946
9	0.5852891529124783	1.22194514708768
>10	10.091767274665814	56.526810802202334
>50	0.8276514799785142	11.655777864987993
>100	0.04403097668518504	1.6638343952277128
>500	0.001257910305750259	0.19442356534637462
>1k	3.5940294450007395E-4	0.0990459672519267
>5k	0.0	0.0
>10k+	1.7970147225003697E-4	0.5064433737978357
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12149	0.49375942385928123	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028449386509300918	0.0
2	0.0	0.0	0.0	0.0991257909945499	0.0
3	0.0	0.0	0.0	0.1599668361437263	0.0
4	0.0	0.0	0.0	0.26120601013611	0.0
5	0.0	0.0	0.0	0.4679924080780001	0.0
6	0.0	0.0	0.0	0.833079320953786	0.0
7	0.0	0.0	0.0	1.0225522351057301	0.0
8	0.0	0.0	0.0	1.3887364814611605	0.0
9	0.0	0.0	0.0	1.5831270752811408	0.0
10	0.0	0.0	0.0	1.8571353093464362	0.0
11	0.0	0.0	0.0	2.148904088989681	0.0
12	0.0	0.0	0.0	2.359795326985056	0.0
13	0.0	0.0	0.0	2.488020776180548	0.0
14	0.0	0.0	0.0	2.5747101210724606	0.0
15	0.0	0.0	0.0	2.6579448976025297	0.0
16	0.0	0.0	0.0	2.7812526671299853	0.0
17	0.0	0.0	0.0	2.9230525378884864	0.0
18	0.0	0.0	0.0	3.081840756591113	0.0
19	0.0	0.0	0.0	3.2000276365468947	0.0
20	0.0	0.0	0.0	3.3213439490186993	0.0
21	0.0	0.0	0.0	3.4718818456336287	0.0
22	0.0	0.0	0.0	3.645585671263275	0.0
23	0.0	0.0	0.0	3.8229066331776744	0.0
24	0.0	0.0	0.0	3.968201714278747	0.0
25	0.0	0.0	0.0	4.098134126664797	0.0
26	0.0	0.0	0.0	4.225790588130103	0.0
27	0.0	0.0	0.0	4.361453519798741	0.0
28	0.0	0.0	0.0	4.499311118426667	0.0
29	0.0	0.0	0.0	4.650824422579059	0.0
30	0.0	0.0	0.0	4.836883410349887	0.0
31	0.0	0.0	0.0	5.0073765195020545	0.0
32	0.0	0.0	0.0	5.181446122958248	0.0
33	0.0	0.0	0.0	5.3509231825922265	0.0
34	0.0	0.0	0.0	5.529178910063361	0.0
35	0.0	0.0	0.0	5.71605073744874	0.0
36	0.0	0.0	0.0	5.8927620696522265	0.0
37	4.064198072757274E-5	0.0	0.0	6.077357946116862	0.0
38	4.064198072757274E-5	0.0	0.0	6.286054517152948	0.0
39	4.064198072757274E-5	0.0	0.0	6.5763601854900005	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7085	0.0	40.236416	1
AGGGCGA	2180	0.0	39.529816	6
GGGCGAT	4370	0.0	38.56407	7
TAGGGAC	2970	0.0	38.560608	5
CGTTAGG	430	0.0	38.197674	2
TATGGGA	3375	0.0	38.066666	4
ACGGGAT	1300	0.0	37.557693	5
ACGCGAA	30	1.1403004E-4	37.500004	14
TAGGGAT	4295	0.0	37.2468	5
TACGGGA	1260	0.0	37.142857	4
AGGGATG	4800	0.0	36.9375	6
ATAGGGA	4030	0.0	36.7928	4
TAAGGGA	4320	0.0	36.666664	4
CGGTAGT	485	0.0	36.649487	12
TTAGGGA	3855	0.0	36.128407	4
TTTAGCG	175	0.0	36.0	1
TGGGCGA	1370	0.0	35.967155	6
GTAGGGA	4040	0.0	35.92203	4
CGAGGGA	1900	0.0	35.881577	4
AGGGACC	3165	0.0	35.829384	6
>>END_MODULE