FastQCFastQC Report
Thu 26 May 2016
SRR1548012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548012_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2047632
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141770.6923607366948749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT207.034579E-445.00000426
CGCTATA207.034579E-445.00000442
TACCGCG406.8193913E-945.0000041
CGTTTTT87700.042.8192671
TATTGCG1500.040.51
AATCGCG451.929584E-840.01
TAGTACG900.040.01
ACGGGAC8800.038.8636365
CGACGGT700.038.5714328
GCGATGC2400.038.4375049
ACGGGAT12050.038.278015
TTTAGCG1650.038.1818161
AACGGGA10150.038.128084
AGGGCGA15950.038.0877766
TTACGAG1300.038.076921
TCGAACG659.094947E-1238.076921
GTTAGCG950.037.8947371
TAAGGGA34300.037.718664
AGGGATT51500.037.66026
AAGGGAT50550.037.5667655