##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548012_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2047632
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.914789864585043	31.0	31.0	33.0	30.0	33.0
2	31.254821178805567	31.0	31.0	33.0	30.0	34.0
3	31.340744821335083	31.0	31.0	34.0	30.0	34.0
4	35.25228361346179	37.0	35.0	37.0	33.0	37.0
5	35.11632510138541	37.0	35.0	37.0	32.0	37.0
6	35.17069033888902	37.0	35.0	37.0	32.0	37.0
7	35.594435914265844	36.0	35.0	37.0	35.0	37.0
8	35.71006606655884	37.0	35.0	37.0	35.0	37.0
9	37.547181329457636	39.0	37.0	39.0	35.0	39.0
10	36.69754233182525	39.0	37.0	39.0	32.0	39.0
11	36.23031140361159	38.0	35.0	39.0	31.0	39.0
12	35.85597705056377	37.0	35.0	39.0	31.0	39.0
13	35.643062815974744	37.0	35.0	39.0	30.0	39.0
14	36.6973176820835	39.0	35.0	40.0	31.0	41.0
15	36.902390175578425	39.0	35.0	41.0	31.0	41.0
16	37.00752771982466	39.0	35.0	41.0	31.0	41.0
17	36.94092248997867	38.0	35.0	41.0	31.0	41.0
18	36.82362065058565	38.0	35.0	40.0	31.0	41.0
19	36.76392877235753	38.0	35.0	40.0	31.0	41.0
20	36.61612731193886	38.0	35.0	40.0	31.0	41.0
21	36.502725099041236	38.0	35.0	40.0	31.0	41.0
22	36.370984630050714	38.0	35.0	40.0	30.0	41.0
23	36.31201749142424	38.0	35.0	40.0	30.0	41.0
24	36.217713436789424	38.0	34.0	40.0	30.0	41.0
25	36.06983188385413	38.0	34.0	40.0	30.0	41.0
26	35.8671455613118	38.0	34.0	40.0	29.0	41.0
27	35.898952546160636	38.0	34.0	40.0	30.0	41.0
28	35.94015282042867	38.0	34.0	40.0	30.0	41.0
29	35.953967802808315	38.0	34.0	40.0	30.0	41.0
30	35.885616653773724	38.0	34.0	40.0	30.0	41.0
31	35.790072141869246	38.0	34.0	40.0	29.0	41.0
32	35.625824366878426	38.0	34.0	40.0	29.0	41.0
33	35.429519562108815	38.0	34.0	40.0	27.0	41.0
34	35.249114098627096	38.0	34.0	40.0	27.0	41.0
35	35.060719894981126	38.0	34.0	40.0	25.0	41.0
36	34.97407053611196	38.0	34.0	40.0	25.0	41.0
37	34.924190967908295	38.0	34.0	40.0	25.0	41.0
38	34.87837658329231	38.0	34.0	40.0	25.0	41.0
39	34.84344696703314	38.0	34.0	40.0	24.0	41.0
40	34.697449541714526	38.0	33.0	40.0	24.0	41.0
41	34.639108980519936	38.0	33.0	40.0	24.0	41.0
42	34.613500375067396	37.0	33.0	40.0	24.0	41.0
43	34.57696109457168	37.0	33.0	40.0	24.0	41.0
44	34.4379107183322	37.0	33.0	40.0	23.0	41.0
45	34.335859177821014	37.0	33.0	40.0	23.0	41.0
46	34.2890778225775	37.0	33.0	40.0	23.0	41.0
47	34.26053363104308	37.0	33.0	40.0	23.0	41.0
48	34.17669581252881	37.0	33.0	40.0	23.0	41.0
49	34.04974135977559	37.0	33.0	40.0	23.0	41.0
50	33.949516319338635	36.0	33.0	40.0	23.0	41.0
51	33.632398302038645	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	12.0
14	18.0
15	80.0
16	173.0
17	450.0
18	999.0
19	1949.0
20	3471.0
21	5589.0
22	8420.0
23	13050.0
24	18805.0
25	27751.0
26	36195.0
27	41246.0
28	45276.0
29	51444.0
30	61564.0
31	74427.0
32	91381.0
33	114896.0
34	166100.0
35	200966.0
36	173192.0
37	216119.0
38	302048.0
39	391979.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.359670096970547	25.70701180680904	30.57697867585582	14.356339420364597
2	30.56335318064965	26.843690663166036	29.396834978160136	13.196121178024175
3	27.20479070457973	25.736216273236597	33.326300819678536	13.732692202505136
4	23.963192604921197	28.710871875415112	32.92539870445471	14.400536815208984
5	22.170634176453582	33.531659985778695	30.955025121701556	13.34268071606617
6	21.25215859099682	41.46404236698782	27.26700891566453	10.016790126350829
7	90.75209803324034	2.5255026293787166	5.908776577041188	0.8136227603397486
8	92.02976902099596	1.662798784156528	5.545918407213796	0.7615137876337154
9	87.50366276752854	4.869625010744118	6.3910409682989915	1.2356712534283503
10	39.000904459395045	41.997292482242905	11.084853137673175	7.916949920688873
11	27.260122912710877	25.60093805918251	31.261476671589428	15.877462356517185
12	28.970928369941472	24.474270767403517	29.73947467122999	16.815326191425022
13	21.904521906280035	30.23404596138369	30.775891371105747	17.085540761230533
14	17.26887448525907	33.878108957078226	30.805095837533308	18.047920720129397
15	16.609087961118014	28.19110074466506	38.87173085788852	16.328080436328403
16	20.606095235862696	26.491283590020082	36.86849004117928	16.03413113293795
17	20.990148620455237	25.735044187627466	30.28923165881369	22.985575533103606
18	21.534826570399368	27.180616438891363	33.05730717238254	18.22724981832673
19	24.65081616227916	27.728175765957946	29.31234714050181	18.308660931261088
20	26.104397665205465	28.728355485751344	28.768206396461864	16.399040452581325
21	23.502807145033874	28.032331981527932	31.252490681919408	17.21237019151879
22	23.053165803230268	25.423367089398873	30.80372840432265	20.719738703048204
23	19.714431108714848	29.84681817826641	30.642859654469163	19.795891058549582
24	19.382340186127195	28.134791798526297	35.1265266415059	17.35634137384061
25	19.426684091672723	29.806039366448662	32.569426537580966	18.197850004297646
26	19.200569242910838	33.828099971088555	28.473182681263037	18.49814810473757
27	18.46362041616853	32.80692038413152	31.37248294615439	17.356976253545557
28	17.144975268993647	30.794009861146925	34.834530814130666	17.226484055728765
29	18.092606483977587	28.66393961414942	34.44593559780273	18.797518304070262
30	19.46174898614595	30.32815466841698	31.893328488712815	18.316767856724255
31	22.901038858544894	29.234598795095994	29.30956343718012	18.55479890917899
32	23.91904404697719	29.192501386967972	29.556971174507918	17.331483391546918
33	22.978884877751472	29.484546051243584	29.429506864514714	18.10706220649023
34	19.54569961790009	30.240834290536583	30.45723059612274	19.75623549544059
35	19.24652476616892	29.96353837017589	31.191981762347922	19.597955101307267
36	23.194890488134586	29.439225407690444	29.06337662236183	18.302507481813137
37	19.907092680716065	32.375641716871	29.75031646311447	17.966949139298467
38	20.05458011986529	32.50935715011291	28.01499488189284	19.421067848128963
39	20.18575603428741	31.77548504809458	29.05058135446213	18.98817756315588
40	21.821743360135024	29.65454730146823	28.3806855919423	20.143023746454443
41	18.431534572618517	29.816490463130098	29.33608187408675	22.41589309016464
42	20.364254905178274	29.73434679669003	28.32540222071153	21.57599607742016
43	20.656104221852363	29.721111996686904	28.494866265031998	21.12791751642873
44	19.87559287997062	31.779880369128826	29.142785422380584	19.20174132851997
45	18.694716628769232	33.930217929784256	27.01100588386976	20.364059557576752
46	20.33373184244044	32.59491939957962	27.502646960000625	19.568701797979323
47	20.04012439735265	30.81203067738734	28.919893809043813	20.227951116216193
48	20.893744579104055	29.480492588511996	30.104042132570697	19.521720699813248
49	20.34755268524813	28.86885924814615	30.41327738578026	20.370310680825458
50	19.191290231838533	32.157584956671904	28.867003445931687	19.784121365557876
51	18.405944036819115	33.05882111629433	27.93231400954859	20.602920837337958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1446.0
1	1685.5
2	1925.0
3	12646.5
4	23368.0
5	17818.0
6	12268.0
7	11552.5
8	10837.0
9	11708.0
10	12579.0
11	13306.0
12	14033.0
13	14398.5
14	14764.0
15	14414.5
16	14065.0
17	13880.5
18	13696.0
19	13734.0
20	13772.0
21	14782.5
22	15793.0
23	16774.0
24	17755.0
25	20682.5
26	28009.5
27	32409.0
28	38862.0
29	45315.0
30	50258.0
31	55201.0
32	62550.0
33	69899.0
34	76526.5
35	83154.0
36	90442.0
37	97730.0
38	106359.0
39	114988.0
40	128301.5
41	141615.0
42	157432.5
43	173250.0
44	173533.5
45	173817.0
46	169492.0
47	165167.0
48	156002.5
49	146838.0
50	139039.5
51	131241.0
52	119820.0
53	108399.0
54	97391.5
55	86384.0
56	77345.5
57	68307.0
58	61151.0
59	53995.0
60	48674.0
61	43353.0
62	35887.5
63	28422.0
64	22636.5
65	16851.0
66	12990.5
67	9130.0
68	7176.0
69	5222.0
70	3941.0
71	2660.0
72	2243.0
73	1826.0
74	1530.5
75	966.0
76	697.0
77	483.5
78	270.0
79	219.0
80	168.0
81	114.5
82	61.0
83	62.5
84	64.0
85	50.0
86	36.0
87	23.5
88	11.0
89	6.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2047632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.264333963072573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03038717359458	17.222676506220687
2	7.72767253049125	3.595583090132211
3	3.1312411964202287	2.1853872273735333
4	1.796727996464905	1.6719872060224727
5	1.2144330983714298	1.4126488588160955
6	0.8771972215833445	1.2244445468636578
7	0.7368566810247646	1.1999735937196956
8	0.5681716739938961	1.0574508457721596
9	0.517125261569129	1.0827517307296928
>10	7.95490268078095	45.57575645182391
>50	1.3637230784617345	20.193282467478305
>100	0.08070060286900822	2.657560525688421
>500	0.0	0.0
>1k	6.456032810809686E-4	0.21072401065228463
>5k	0.0	0.0
>10k+	2.152010936936562E-4	0.709772938706923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14177	0.6923607366948749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.883690038053713E-5	0.0	0.0	0.011525508489806761	0.0
2	4.883690038053713E-5	0.0	0.0	0.04180438672573978	0.0
3	4.883690038053713E-5	0.0	0.0	0.0831204044476742	0.0
4	4.883690038053713E-5	0.0	0.0	0.13112707752174219	0.0
5	4.883690038053713E-5	0.0	0.0	0.2362240871406581	0.0
6	4.883690038053713E-5	0.0	0.0	0.4036858185455199	0.0
7	4.883690038053713E-5	0.0	0.0	0.5069758628503559	0.0
8	4.883690038053713E-5	0.0	0.0	0.6902607499785117	0.0
9	4.883690038053713E-5	0.0	0.0	0.7863717699274089	0.0
10	4.883690038053713E-5	0.0	0.0	0.9165709463419208	0.0
11	4.883690038053713E-5	0.0	0.0	1.0931163412175626	0.0
12	4.883690038053713E-5	0.0	0.0	1.2301038467849692	0.0
13	4.883690038053713E-5	0.0	0.0	1.3053126733709963	0.0
14	4.883690038053713E-5	0.0	0.0	1.3535146940465865	0.0
15	4.883690038053713E-5	0.0	0.0	1.400837650515327	0.0
16	4.883690038053713E-5	0.0	0.0	1.4779511162161951	0.0
17	4.883690038053713E-5	0.0	0.0	1.585782992256421	0.0
18	4.883690038053713E-5	0.0	0.0	1.6977660048289926	0.0
19	4.883690038053713E-5	0.0	0.0	1.7799585081694367	0.0
20	4.883690038053713E-5	0.0	0.0	1.8596114926900928	0.0
21	4.883690038053713E-5	0.0	0.0	1.9626085155926456	0.0
22	4.883690038053713E-5	0.0	0.0	2.0825519429272448	0.0
23	4.883690038053713E-5	0.0	0.0	2.2141673894527925	0.0
24	4.883690038053713E-5	0.0	0.0	2.316090000546973	0.0
25	4.883690038053713E-5	0.0	0.0	2.404631300936887	0.0
26	4.883690038053713E-5	0.0	0.0	2.4912191253115794	0.0
27	4.883690038053713E-5	0.0	0.0	2.587769677363901	0.0
28	4.883690038053713E-5	0.0	0.0	2.693208545285481	0.0
29	4.883690038053713E-5	0.0	0.0	2.809049672988115	0.0
30	4.883690038053713E-5	0.0	0.0	2.9457441571532383	0.0
31	4.883690038053713E-5	0.0	0.0	3.075796822866609	0.0
32	4.883690038053713E-5	0.0	0.0	3.1965216406072967	0.0
33	4.883690038053713E-5	0.0	0.0	3.31866272845902	0.0
34	4.883690038053713E-5	0.0	0.0	3.4489107417739127	0.0
35	4.883690038053713E-5	0.0	0.0	3.597326082030365	0.0
36	4.883690038053713E-5	0.0	0.0	3.744569336677684	0.0
37	4.883690038053713E-5	0.0	0.0	3.8972334872672434	0.0
38	4.883690038053713E-5	0.0	0.0	4.050190659259085	0.0
39	4.883690038053713E-5	0.0	0.0	4.254182392148589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAAT	20	7.034579E-4	45.000004	26
CGCTATA	20	7.034579E-4	45.000004	42
TACCGCG	40	6.8193913E-9	45.000004	1
CGTTTTT	8770	0.0	42.819267	1
TATTGCG	150	0.0	40.5	1
AATCGCG	45	1.929584E-8	40.0	1
TAGTACG	90	0.0	40.0	1
ACGGGAC	880	0.0	38.863636	5
CGACGGT	70	0.0	38.57143	28
GCGATGC	240	0.0	38.437504	9
ACGGGAT	1205	0.0	38.27801	5
TTTAGCG	165	0.0	38.181816	1
AACGGGA	1015	0.0	38.12808	4
AGGGCGA	1595	0.0	38.087776	6
TTACGAG	130	0.0	38.07692	1
TCGAACG	65	9.094947E-12	38.07692	1
GTTAGCG	95	0.0	37.894737	1
TAAGGGA	3430	0.0	37.71866	4
AGGGATT	5150	0.0	37.6602	6
AAGGGAT	5055	0.0	37.566765	5
>>END_MODULE