FastQCFastQC Report
Thu 26 May 2016
SRR1548011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548011_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3115588
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172500.5536675580981824No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA207.035147E-445.010
CGTTTTT105050.041.722991
TATCACG850.039.7058831
CGAGGGA26450.039.640834
CGTAAGG5000.039.6000022
GCGTAAG1900.039.078951
TACGGGA14950.038.829434
AGGGATT79900.038.4949956
GCGCGAC1650.038.1818169
TAGGGAC44850.038.1772545
AAGGGAC46600.038.1437765
GGGCGAT52150.038.0105487
TAAGGGA51350.037.989294
ACGGGAC11750.037.9148985
ATAGGGA55300.037.8390584
TAACGGG10250.037.75613
GGCGATT16300.037.6840488
AAGGGAT74550.037.4849135
TTAGGGA55250.037.425344
GGACCGA24650.037.4239358
Produced by FastQC (version 0.11.5)