##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548011_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3115588
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86530921289978	31.0	31.0	31.0	30.0	33.0
2	31.20210053447375	31.0	31.0	33.0	30.0	34.0
3	31.336657478459923	31.0	31.0	34.0	30.0	34.0
4	35.23285813143458	37.0	35.0	37.0	33.0	37.0
5	35.07211768693421	35.0	35.0	37.0	32.0	37.0
6	35.11683861922693	37.0	35.0	37.0	32.0	37.0
7	35.55696099741044	36.0	35.0	37.0	35.0	37.0
8	35.66541307772401	37.0	35.0	37.0	35.0	37.0
9	37.49067976895533	39.0	37.0	39.0	35.0	39.0
10	36.59125564740909	39.0	35.0	39.0	32.0	39.0
11	36.11280438877027	38.0	35.0	39.0	31.0	39.0
12	35.73053529542417	37.0	35.0	39.0	30.0	39.0
13	35.53536154331061	37.0	35.0	39.0	30.0	39.0
14	36.54914160665659	38.0	35.0	40.0	30.0	41.0
15	36.751061757844745	38.0	35.0	40.0	31.0	41.0
16	36.8441331138777	38.0	35.0	40.0	31.0	41.0
17	36.769854679116754	38.0	35.0	40.0	31.0	41.0
18	36.672994953119606	38.0	35.0	40.0	31.0	41.0
19	36.614056158901626	38.0	35.0	40.0	31.0	41.0
20	36.48943506009139	38.0	35.0	40.0	30.0	41.0
21	36.37776881924054	38.0	35.0	40.0	30.0	41.0
22	36.261934504818996	38.0	34.0	40.0	30.0	41.0
23	36.202229563087286	38.0	34.0	40.0	30.0	41.0
24	36.10377206485582	38.0	34.0	40.0	30.0	41.0
25	35.95614375199802	38.0	34.0	40.0	30.0	41.0
26	35.72985580891954	38.0	34.0	40.0	29.0	41.0
27	35.787400002824505	38.0	34.0	40.0	29.0	41.0
28	35.80924371258331	38.0	34.0	40.0	29.0	41.0
29	35.822743251033195	38.0	34.0	40.0	29.0	41.0
30	35.73827829610334	38.0	34.0	40.0	29.0	41.0
31	35.651269680073234	38.0	34.0	40.0	29.0	41.0
32	35.53748024449959	38.0	34.0	40.0	28.0	41.0
33	35.36918520677317	38.0	34.0	40.0	27.0	41.0
34	35.21743439761612	38.0	34.0	40.0	26.0	41.0
35	35.06757761295781	38.0	34.0	40.0	25.0	41.0
36	34.99120037694329	38.0	34.0	40.0	25.0	41.0
37	34.95414605525506	38.0	34.0	40.0	25.0	41.0
38	34.8792118855253	38.0	33.0	40.0	25.0	41.0
39	34.86400063166246	38.0	33.0	40.0	25.0	41.0
40	34.721663775826585	38.0	33.0	40.0	24.0	41.0
41	34.66592052607726	37.0	33.0	40.0	24.0	41.0
42	34.62944651218326	37.0	33.0	40.0	24.0	41.0
43	34.59872325865936	37.0	33.0	40.0	24.0	41.0
44	34.42748046275695	37.0	33.0	40.0	23.0	41.0
45	34.31081901714861	37.0	33.0	40.0	23.0	41.0
46	34.274745569696634	37.0	33.0	40.0	23.0	41.0
47	34.2399104117746	37.0	33.0	40.0	23.0	41.0
48	34.170143805920425	37.0	33.0	40.0	23.0	41.0
49	34.05541393791477	36.0	33.0	40.0	23.0	41.0
50	33.951135066639104	36.0	33.0	40.0	23.0	41.0
51	33.632258501444994	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	9.0
14	33.0
15	115.0
16	323.0
17	807.0
18	1699.0
19	3237.0
20	5513.0
21	8924.0
22	13554.0
23	19687.0
24	29089.0
25	40742.0
26	53892.0
27	62716.0
28	70621.0
29	82637.0
30	98407.0
31	118429.0
32	143312.0
33	180142.0
34	257907.0
35	299939.0
36	263324.0
37	325849.0
38	453433.0
39	581196.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.485256715586274	25.756422222707236	30.0870975238061	13.67122353790039
2	29.613896317484855	26.725837947764596	30.222577568022473	13.43768816672808
3	27.632633069584294	26.721023447259395	32.256607741460044	13.38973574169627
4	24.2786915343107	29.040842370685727	32.21693625729718	14.463529837706398
5	22.414677422046818	33.847190321698505	30.195584268523305	13.542547987731368
6	21.30724601584035	41.922616212413196	26.58727662322489	10.182861148521564
7	91.56842945858054	2.3438593292823056	5.223989821503999	0.8637213906331647
8	92.71501880222931	1.6472652995197055	4.752874898735006	0.8848409995159823
9	88.06270277071295	4.863897280384954	5.6927295906904245	1.3806703582116764
10	40.473900913728	40.92299752085321	10.454495267025036	8.148606298393753
11	27.69778930975469	24.926947979001074	30.44593829479379	16.92932441645044
12	28.982875784603102	23.895200520736378	29.140470434473364	17.98145326018716
13	22.888969915149243	29.013913264526632	30.043927502609456	18.053189317714665
14	17.89607611789492	32.34381439394426	30.885502190918697	18.874607297242125
15	17.398481442347318	28.295493499140452	37.66971114280835	16.636313915703873
16	21.593933472590084	26.448939975375435	35.47417052575629	16.48295602627819
17	21.360879551468294	25.22804684059638	30.31713435794463	23.09393924999069
18	22.12166692130025	26.461746546719272	32.515820448660094	18.900766083320388
19	24.537840048170683	27.50565864292711	29.057372155753587	18.89912915314862
20	26.24050420017024	28.05001816671524	28.646438489299612	17.063039143814905
21	23.948673573014148	27.382632106684195	30.920519657926533	17.748174662375128
22	23.732181533630246	24.767844785639177	30.691413627219006	20.808560053511567
23	20.305476847388036	28.364982789765524	30.770403532174345	20.55913683067209
24	20.546811709378776	27.394796744627335	34.35528060834744	17.70311093764644
25	20.66380407165517	28.659951187384213	31.445460696343673	19.23078404461694
26	19.62579776273371	32.52904427671438	28.296488495911525	19.548669464640383
27	18.643414982982346	31.78626313877188	30.781348496656168	18.788973381589607
28	17.702725777606023	30.23984557650113	33.53472923891092	18.522699406981925
29	19.207834925542144	27.639598046981824	33.17267880091976	19.979888226556273
30	19.961111674585986	29.092999459492074	31.68374637468112	19.26214249124082
31	23.506670329966607	28.218140524356876	29.344187999183465	18.931001146493053
32	24.09265923478971	28.344793984313714	29.219267759408496	18.34327902148808
33	23.17228722154534	28.977868704077693	29.172727587858216	18.67711648651876
34	20.25258153517089	28.892170595085105	30.26606855591946	20.58917931382455
35	19.87419389213208	29.264941320867845	30.8863367043396	19.97452808266048
36	23.333605085139627	28.901927982775643	29.13803108755073	18.626435844534
37	20.84377010053961	30.74764699311976	29.756084565738472	18.652498340602158
38	20.16852035635007	31.390382810564173	27.990896100511364	20.450200732574398
39	20.29405043285569	30.55696067644374	29.240772528331732	19.908216362368837
40	22.31498516491911	28.583143855991228	28.31767229813441	20.78419868095525
41	18.792183048593074	28.752389597084083	29.676516920722506	22.778910433600334
42	20.295719459697494	28.568925031165865	28.801561695577206	22.33379381355943
43	20.69484155157871	28.18450321416054	29.11463261509545	22.006022619165307
44	19.976325496182422	30.071819508869595	28.976937900646682	20.9749170943013
45	19.228537277714512	31.751309865104115	27.888989173151263	21.13116368403011
46	21.289079300600722	30.7733243291475	28.27938097078304	19.658215399468737
47	20.265259719834585	29.509004399811527	29.294759127330057	20.930976753023828
48	20.961211816196492	28.277936620631483	30.395065072788828	20.365786490383194
49	20.93421851669733	27.869217624409902	30.115021626736272	21.081542232156497
50	19.61905746202643	30.473477237683543	29.383955773356423	20.5235095269336
51	19.22288826378841	30.99032991525195	28.268596489651394	21.51818533130825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1987.0
1	2431.0
2	2875.0
3	16422.0
4	29969.0
5	22891.0
6	15813.0
7	14691.0
8	13569.0
9	14230.0
10	14891.0
11	15724.0
12	16557.0
13	16645.5
14	16734.0
15	16193.0
16	15652.0
17	15408.5
18	15165.0
19	15535.5
20	15906.0
21	16446.5
22	16987.0
23	20884.0
24	24781.0
25	28753.5
26	37958.5
27	43191.0
28	52114.5
29	61038.0
30	72761.5
31	84485.0
32	95902.0
33	107319.0
34	118062.5
35	128806.0
36	139174.0
37	149542.0
38	161514.0
39	173486.0
40	193123.0
41	212760.0
42	227116.0
43	241472.0
44	248719.0
45	255966.0
46	250906.0
47	245846.0
48	240003.5
49	234161.0
50	217912.5
51	201664.0
52	186880.5
53	172097.0
54	158535.5
55	144974.0
56	130953.5
57	116933.0
58	105088.0
59	93243.0
60	84954.0
61	76665.0
62	65699.5
63	54734.0
64	45666.0
65	36598.0
66	28905.5
67	21213.0
68	15979.5
69	10746.0
70	8539.0
71	6332.0
72	5006.5
73	3681.0
74	2953.0
75	1892.5
76	1560.0
77	1048.0
78	536.0
79	425.0
80	314.0
81	249.5
82	185.0
83	153.0
84	121.0
85	78.5
86	36.0
87	31.0
88	26.0
89	21.5
90	17.0
91	9.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3115588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.97708705423148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59718396581214	15.301886121940125
2	7.19568268199188	2.8749755870555527
3	2.594329724436092	1.5548145225732053
4	1.5061073134468996	1.2035054765497366
5	0.9392431275112949	0.9381670861690888
6	0.7281281223654562	0.8727527332277298
7	0.564406318407539	0.7892635909749989
8	0.4511091943420693	0.7209478117068597
9	0.3829133452416715	0.6884543908907877
>10	6.273056103530301	31.997721598605317
>50	2.4438819028685077	33.90966601778832
>100	0.32263482257265763	8.285132252617284
>500	6.616887367718445E-4	0.10498883770227027
>1k	4.962665525788833E-4	0.1876712651278542
>5k	0.0	0.0
>10k+	1.6542218419296113E-4	0.5700527070708725
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17250	0.5536675580981824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015534788296783786	0.0
2	0.0	0.0	0.0	0.05828755278297387	0.0
3	0.0	0.0	0.0	0.09086567286817128	0.0
4	0.0	0.0	0.0	0.15284434270513303	0.0
5	0.0	0.0	0.0	0.2840234331368589	0.0
6	0.0	0.0	0.0	0.49538000531520854	0.0
7	0.0	0.0	0.0	0.6231889453932934	0.0
8	0.0	0.0	0.0	0.8740886150543653	0.0
9	0.0	0.0	0.0	0.99345613091333	0.0
10	0.0	0.0	0.0	1.1544209311372364	0.0
11	0.0	0.0	0.0	1.3611555828305926	0.0
12	0.0	0.0	0.0	1.5127160587343385	0.0
13	0.0	0.0	0.0	1.591705963689679	0.0
14	0.0	0.0	0.0	1.6407817721727007	0.0
15	0.0	0.0	0.0	1.6932919243494327	0.0
16	0.0	0.0	0.0	1.7907695112447475	0.0
17	0.0	0.0	0.0	1.9188031280130748	0.0
18	0.0	0.0	0.0	2.0538017221789273	0.0
19	0.0	0.0	0.0	2.1514718890944504	0.0
20	0.0	0.0	0.0	2.2486285092894183	0.0
21	0.0	0.0	0.0	2.371173595481816	0.0
22	0.0	0.0	0.0	2.5273559918705555	0.0
23	0.0	0.0	0.0	2.6863307985523117	0.0
24	0.0	0.0	0.0	2.814139738630397	0.0
25	0.0	0.0	0.0	2.9259324403611773	0.0
26	0.0	0.0	0.0	3.039843522314247	0.0
27	0.0	0.0	0.0	3.1622281251564712	0.0
28	3.209667003467724E-5	0.0	0.0	3.2934072155881973	0.0
29	3.209667003467724E-5	0.0	0.0	3.4373928773637594	0.0
30	3.209667003467724E-5	0.0	0.0	3.6123197290527504	0.0
31	3.209667003467724E-5	0.0	0.0	3.7713908257446107	0.0
32	3.209667003467724E-5	0.0	0.0	3.923208075008634	0.0
33	3.209667003467724E-5	0.0	0.0	4.073356297430854	0.0
34	3.209667003467724E-5	0.0	0.0	4.2339038409443095	0.0
35	3.209667003467724E-5	0.0	0.0	4.420385493845784	0.0
36	3.209667003467724E-5	0.0	0.0	4.596146858955677	0.0
37	3.209667003467724E-5	0.0	0.0	4.782853188547395	0.0
38	3.209667003467724E-5	0.0	0.0	4.984580759715341	0.0
39	6.419334006935449E-5	0.0	0.0	5.256279071558884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	20	7.035147E-4	45.0	10
CGTTTTT	10505	0.0	41.72299	1
TATCACG	85	0.0	39.705883	1
CGAGGGA	2645	0.0	39.64083	4
CGTAAGG	500	0.0	39.600002	2
GCGTAAG	190	0.0	39.07895	1
TACGGGA	1495	0.0	38.82943	4
AGGGATT	7990	0.0	38.494995	6
GCGCGAC	165	0.0	38.181816	9
TAGGGAC	4485	0.0	38.177254	5
AAGGGAC	4660	0.0	38.143776	5
GGGCGAT	5215	0.0	38.010548	7
TAAGGGA	5135	0.0	37.98929	4
ACGGGAC	1175	0.0	37.914898	5
ATAGGGA	5530	0.0	37.839058	4
TAACGGG	1025	0.0	37.7561	3
GGCGATT	1630	0.0	37.684048	8
AAGGGAT	7455	0.0	37.484913	5
TTAGGGA	5525	0.0	37.42534	4
GGACCGA	2465	0.0	37.423935	8
>>END_MODULE