FastQCFastQC Report
Thu 26 May 2016
SRR1548010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548010_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3333396
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151250.4537414696603704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96500.041.1994821
AGGGCGA31750.039.614176
TACGGGA15100.039.1887444
CGTTAGG5400.039.1666682
TAAGGGA56100.038.6229934
GCGAGAC5300.037.7830221
GGGCGAT58550.037.7754067
TAGGGAC43400.037.638255
ACGGGAT18900.037.6190455
AAGGGAT67800.037.4336285
TTAGGGA56050.037.2925954
ATAGGGA64150.037.248644
AACGGGA17200.036.8895344
AGGGATT70100.036.815266
GACCGAT21650.036.685919
TAGGGAT69750.036.6774185
GGCGATA13550.036.365318
CACGACC5450.036.33027327
AGGGATG78900.036.3022846
GCGCGAC2550.036.176479
Produced by FastQC (version 0.11.5)