##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548010_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3333396
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91138466596828	31.0	31.0	31.0	30.0	33.0
2	31.259802615710825	31.0	31.0	33.0	30.0	34.0
3	31.407780833720327	31.0	31.0	34.0	30.0	34.0
4	35.30798230993258	37.0	35.0	37.0	33.0	37.0
5	35.159737696931295	37.0	35.0	37.0	32.0	37.0
6	35.19863466566829	37.0	35.0	37.0	32.0	37.0
7	35.558492900333476	36.0	35.0	37.0	35.0	37.0
8	35.65112665881881	37.0	35.0	37.0	35.0	37.0
9	37.4879495265489	39.0	37.0	39.0	35.0	39.0
10	36.62784829645203	39.0	37.0	39.0	32.0	39.0
11	36.2607517978662	38.0	35.0	39.0	31.0	39.0
12	35.91779854538735	37.0	35.0	39.0	31.0	39.0
13	35.76647389029086	37.0	35.0	39.0	30.0	39.0
14	36.79284309454982	39.0	35.0	40.0	31.0	41.0
15	36.9436496593864	39.0	35.0	40.0	31.0	41.0
16	37.004740810872754	39.0	35.0	41.0	31.0	41.0
17	36.93161838557435	38.0	35.0	40.0	31.0	41.0
18	36.82636866426911	38.0	35.0	40.0	31.0	41.0
19	36.77594291227325	38.0	35.0	40.0	31.0	41.0
20	36.663948417769745	38.0	35.0	40.0	31.0	41.0
21	36.556536037122505	38.0	35.0	40.0	30.0	41.0
22	36.45426285985823	38.0	35.0	40.0	30.0	41.0
23	36.36601561890637	38.0	35.0	40.0	30.0	41.0
24	36.263654543294585	38.0	35.0	40.0	30.0	41.0
25	36.09811495543884	38.0	34.0	40.0	30.0	41.0
26	35.88660363185172	38.0	34.0	40.0	29.0	41.0
27	35.98169404415197	38.0	34.0	40.0	30.0	41.0
28	36.01402683629548	38.0	34.0	40.0	30.0	41.0
29	36.0165743884015	38.0	34.0	40.0	30.0	41.0
30	35.93411043872375	38.0	34.0	40.0	29.0	41.0
31	35.87196240710674	38.0	34.0	40.0	29.0	41.0
32	35.75935892405223	38.0	34.0	40.0	29.0	41.0
33	35.62816179055834	38.0	34.0	40.0	28.0	41.0
34	35.46590384100779	38.0	34.0	40.0	27.0	41.0
35	35.31585896185152	38.0	34.0	40.0	26.0	41.0
36	35.25705346739481	38.0	34.0	40.0	26.0	41.0
37	35.22325610278527	38.0	34.0	40.0	26.0	41.0
38	35.164717303314696	38.0	34.0	40.0	26.0	41.0
39	35.12746790360341	38.0	34.0	40.0	26.0	41.0
40	34.97993457722995	38.0	34.0	40.0	25.0	41.0
41	34.89908309723777	38.0	34.0	40.0	25.0	41.0
42	34.856303901486655	38.0	33.0	40.0	25.0	41.0
43	34.80718102499673	38.0	33.0	40.0	24.0	41.0
44	34.670277398784904	37.0	33.0	40.0	24.0	41.0
45	34.565036377316105	37.0	33.0	40.0	24.0	41.0
46	34.508513239951085	37.0	33.0	40.0	24.0	41.0
47	34.45345887497315	37.0	33.0	40.0	24.0	41.0
48	34.37211270428116	37.0	33.0	40.0	23.0	41.0
49	34.23566896942337	37.0	33.0	40.0	24.0	41.0
50	34.13368348675045	37.0	33.0	40.0	24.0	41.0
51	33.80265951000121	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	3.0
13	11.0
14	31.0
15	107.0
16	296.0
17	707.0
18	1588.0
19	3042.0
20	5304.0
21	8215.0
22	12882.0
23	18769.0
24	27540.0
25	39129.0
26	51984.0
27	61763.0
28	70445.0
29	83412.0
30	99865.0
31	122459.0
32	150357.0
33	189325.0
34	268280.0
35	308071.0
36	294106.0
37	369963.0
38	515490.0
39	630188.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.076685758307743	25.12275769215539	29.835129099572928	13.96542744996394
2	29.729681081995658	26.55907068947104	30.354299339172425	13.356948889360881
3	28.083792024709936	26.28001593570041	32.13752581451469	13.498666225074968
4	24.947950978521604	28.872357199684647	31.856731093455444	14.322960728338307
5	22.669493813516304	33.28407425940392	30.253051242636637	13.793380684443132
6	21.404597593565242	41.552548812082335	26.74578717920103	10.297066415151395
7	91.45103072062246	2.5867613688862647	4.996646063054015	0.9655618474372681
8	92.33622407898731	1.9026242306644636	4.6385727948314575	1.1225788955167644
9	87.33411811857937	5.023705554335579	5.8439501337374855	1.798226193347565
10	36.78245848978039	40.677975254065224	11.960025151527152	10.579541104627232
11	28.901066659946796	25.36275318024021	28.07752214258372	17.658658017229275
12	29.562344227928516	23.161964555066366	28.137611012912956	19.138080204092166
13	24.55825830474387	26.308215405550378	29.345478305007866	19.788047984697887
14	19.687519874626357	29.52388495096292	29.879318268816547	20.909276905594172
15	19.491353562553023	27.175919092721056	34.59066969540972	18.742057649316195
16	23.699104456836213	26.170847988057826	32.468059600479506	17.661987954626454
17	23.246142972512114	25.792105108423964	28.59363243971013	22.368119479353787
18	23.47776861794998	26.082019658030433	30.892969212178812	19.547242511840775
19	25.592308864593345	27.308216605527818	28.01086339576816	19.08861113411068
20	27.19004882708205	27.667489851190798	27.23660795177051	17.905853369956645
21	25.53548993278926	26.44564282191495	29.591383681986777	18.42748356330901
22	24.998770023123566	23.854531534807148	29.988306219843068	21.158392222226222
23	22.43792816695046	27.331526167308056	29.635962843898533	20.59458282184295
24	22.085494792697897	26.384923963429486	32.05787731190654	19.47170393196608
25	22.068815106276002	27.972674113726665	29.557274323242723	20.401236456754614
26	21.55744472003926	30.688103063662403	27.475853453955068	20.278598762343268
27	20.27616880802641	29.611603301857926	29.993646119452954	20.11858177066271
28	19.190549217674707	28.98194513943138	31.643945093832237	20.183560549061678
29	20.61111251108479	26.761356886490535	31.467578409525903	21.159952192898775
30	21.784930443307665	27.23222803411296	30.810920754689814	20.171920767889564
31	23.647625424642015	27.141869732849024	28.585592590859292	20.62491225164967
32	24.239514297131215	27.159509401223257	28.970965345851496	19.63001095579403
33	23.94785978023613	27.58181146194452	28.141990930570504	20.32833782724885
34	20.987455435837806	27.800807344821916	30.035915324791894	21.17582189454838
35	20.95790599136736	28.23993908914512	30.028895456765415	20.773259462722102
36	23.2766523989349	27.95170450795525	28.471084743606824	20.300558349503028
37	21.726131548726883	29.206190923610638	28.80746841959371	20.260209108068768
38	21.448066776344604	29.422096864578943	28.007743454423057	21.122092904653393
39	21.391757834952703	28.65111135910645	28.737689731433047	21.2194410745078
40	22.938438757351364	27.289196963097094	28.511163990116984	21.26120028943456
41	20.41944611441305	27.054961366726303	29.45158631017737	23.074006208683276
42	21.746771160702178	27.64271031704604	28.220679451226317	22.389839071025463
43	21.83784944843037	27.205018545651345	28.33824724095187	22.618884764966417
44	21.121762910857274	28.389486277657983	28.611092111468306	21.87765870001644
45	20.75834974302483	28.82231814041896	27.924795013853736	22.49453710270247
46	21.72295160850976	28.94480583765025	28.076892154427497	21.25535039941249
47	21.01019500833384	27.984463892078832	29.29942917073159	21.705911928855738
48	21.888908488520418	27.079740900871062	29.577733938601952	21.453616672006568
49	21.624613457267003	26.790486338856827	29.437126582020255	22.147773621855908
50	20.867127697999276	28.480744562002236	29.15330191792394	21.498825822074544
51	20.29062853618352	28.635541651816947	28.529493645519466	22.54433616648007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1848.0
1	2338.0
2	2828.0
3	15478.5
4	28129.0
5	21514.0
6	14899.0
7	13939.5
8	12980.0
9	13166.0
10	13352.0
11	13664.5
12	13977.0
13	14013.5
14	14050.0
15	13576.5
16	13103.0
17	12718.5
18	12334.0
19	13065.0
20	13796.0
21	14741.5
22	15687.0
23	18165.0
24	20643.0
25	23751.5
26	33051.5
27	39243.0
28	48900.0
29	58557.0
30	66045.0
31	73533.0
32	84953.0
33	96373.0
34	108500.5
35	120628.0
36	128861.5
37	137095.0
38	151437.0
39	165779.0
40	183126.0
41	200473.0
42	216548.0
43	232623.0
44	241775.0
45	250927.0
46	254840.5
47	258754.0
48	256895.5
49	255037.0
50	244531.5
51	234026.0
52	222236.0
53	210446.0
54	193493.5
55	176541.0
56	164176.5
57	151812.0
58	141855.5
59	131899.0
60	121676.5
61	111454.0
62	95732.5
63	80011.0
64	67006.0
65	54001.0
66	44345.0
67	34689.0
68	28443.5
69	22198.0
70	17510.0
71	12822.0
72	10547.0
73	8272.0
74	6704.0
75	4278.0
76	3420.0
77	2472.5
78	1525.0
79	1122.0
80	719.0
81	606.5
82	494.0
83	340.5
84	187.0
85	165.5
86	144.0
87	90.5
88	37.0
89	35.5
90	34.0
91	24.5
92	15.0
93	10.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3333396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.93890134232022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85116868093777	15.123889690289074
2	6.655811140484412	2.6541912336646902
3	2.3590794551929712	1.4111235754736156
4	1.4041767650241364	1.1199096793997847
5	0.963501020481287	0.9605575895300619
6	0.7291848414656729	0.8723486768579677
7	0.5890497426593546	0.8221503293222792
8	0.5267418654254462	0.840212327007015
9	0.44856975335130483	0.8049589251498522
>10	8.104002096082741	41.80558540222853
>50	2.2481070393862184	29.528312088166402
>100	0.11859660452121751	3.302010990502831
>500	0.0013922272990189886	0.16239297415316367
>1k	4.640757663396611E-4	0.1258429885175509
>5k	0.0	0.0
>10k+	1.5469192211322038E-4	0.4665135297372444
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15125	0.4537414696603704	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02456953809268386	0.0
2	0.0	0.0	0.0	0.0749385911544863	0.0
3	0.0	0.0	0.0	0.11723779592943653	0.0
4	0.0	0.0	0.0	0.18350655007685857	0.0
5	0.0	0.0	0.0	0.33164376509721616	0.0
6	0.0	0.0	0.0	0.5944988234221197	0.0
7	0.0	0.0	0.0	0.7342061969234979	0.0
8	0.0	0.0	0.0	1.0241207465299653	0.0
9	0.0	0.0	0.0	1.1805078064532386	0.0
10	0.0	0.0	0.0	1.398033716966121	0.0
11	0.0	0.0	0.0	1.6414491407561538	0.0
12	0.0	0.0	0.0	1.8290356141304545	0.0
13	0.0	0.0	0.0	1.940903511013993	0.0
14	0.0	0.0	0.0	2.0107721974826873	0.0
15	0.0	0.0	0.0	2.0780909318904803	0.0
16	0.0	0.0	0.0	2.1817089838711032	0.0
17	0.0	0.0	0.0	2.3029067053539394	0.0
18	0.0	0.0	0.0	2.4441740495278688	0.0
19	0.0	0.0	0.0	2.5486920845888097	0.0
20	0.0	0.0	0.0	2.6554900767865566	0.0
21	0.0	0.0	0.0	2.788387578313528	0.0
22	0.0	0.0	0.0	2.949484549690466	0.0
23	0.0	0.0	0.0	3.124621257120366	0.0
24	0.0	0.0	0.0	3.260488702812387	0.0
25	0.0	0.0	0.0	3.3895762759660117	0.0
26	0.0	0.0	0.0	3.509334024520339	0.0
27	0.0	0.0	0.0	3.6392015830102395	0.0
28	0.0	0.0	0.0	3.7694591341682777	0.0
29	0.0	0.0	0.0	3.9176563480606563	0.0
30	0.0	0.0	0.0	4.088323139524977	0.0
31	0.0	0.0	0.0	4.243630219751869	0.0
32	0.0	0.0	0.0	4.402597231172054	0.0
33	0.0	0.0	0.0	4.558444301247136	0.0
34	0.0	0.0	0.0	4.721611233708806	0.0
35	0.0	0.0	0.0	4.895637962006314	0.0
36	0.0	0.0	0.0	5.066364752342656	0.0
37	0.0	0.0	0.0	5.251281275912013	0.0
38	0.0	0.0	0.0	5.449487549634067	0.0
39	0.0	0.0	0.0	5.717112518284656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9650	0.0	41.199482	1
AGGGCGA	3175	0.0	39.61417	6
TACGGGA	1510	0.0	39.188744	4
CGTTAGG	540	0.0	39.166668	2
TAAGGGA	5610	0.0	38.622993	4
GCGAGAC	530	0.0	37.78302	21
GGGCGAT	5855	0.0	37.775406	7
TAGGGAC	4340	0.0	37.63825	5
ACGGGAT	1890	0.0	37.619045	5
AAGGGAT	6780	0.0	37.433628	5
TTAGGGA	5605	0.0	37.292595	4
ATAGGGA	6415	0.0	37.24864	4
AACGGGA	1720	0.0	36.889534	4
AGGGATT	7010	0.0	36.81526	6
GACCGAT	2165	0.0	36.68591	9
TAGGGAT	6975	0.0	36.677418	5
GGCGATA	1355	0.0	36.36531	8
CACGACC	545	0.0	36.330273	27
AGGGATG	7890	0.0	36.302284	6
GCGCGAC	255	0.0	36.17647	9
>>END_MODULE