FastQCFastQC Report
Thu 26 May 2016
SRR1548009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548009_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3391592
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183060.5397465261151696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102400.042.0336951
TATAGCG2900.041.896551
TAACGCG700.041.7857171
CGACCCG1750.039.85714332
AGGGCGA25650.039.3859636
TAATGCG2000.039.3750041
TACGGGA17250.039.04
ACGGGAT22500.038.85
CGTTCGA356.251894E-638.5714314
ACGGGTC700.038.571435
GGGCGAT56750.038.2202647
TAAACGG3300.038.1818162
CGTTAGG5200.038.076922
GCGTAAG2800.037.767861
TAGGGAT75500.037.7284745
TAGGGAC50600.037.7075085
ATAGGGA63800.037.699844
TAAGGGA61900.037.6575134
TTAGGGA57750.037.519484
CCGCTCG301.1403914E-437.50000419
Produced by FastQC (version 0.11.5)