##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548009_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3391592
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.806509745275964	31.0	31.0	31.0	30.0	33.0
2	31.144937539656894	31.0	31.0	33.0	30.0	34.0
3	31.274935192676477	31.0	31.0	34.0	28.0	34.0
4	35.15633661124333	35.0	35.0	37.0	32.0	37.0
5	34.9787987470191	35.0	35.0	37.0	32.0	37.0
6	35.01174846502763	36.0	35.0	37.0	32.0	37.0
7	35.516359279064226	35.0	35.0	37.0	35.0	37.0
8	35.62742865297477	37.0	35.0	37.0	35.0	37.0
9	37.426454007439574	39.0	37.0	39.0	35.0	39.0
10	36.50583590243166	38.0	35.0	39.0	32.0	39.0
11	36.03099930652036	38.0	35.0	39.0	31.0	39.0
12	35.6385473252679	37.0	35.0	39.0	30.0	39.0
13	35.42257765674645	37.0	35.0	39.0	30.0	39.0
14	36.41897285994306	38.0	35.0	40.0	30.0	41.0
15	36.628917924089926	38.0	35.0	40.0	30.0	41.0
16	36.74396890899613	38.0	35.0	40.0	31.0	41.0
17	36.67272272136507	38.0	35.0	40.0	31.0	41.0
18	36.575465739982874	38.0	35.0	40.0	31.0	41.0
19	36.524916322482184	38.0	35.0	40.0	30.0	41.0
20	36.40859012522733	38.0	35.0	40.0	30.0	41.0
21	36.28096274551892	38.0	34.0	40.0	30.0	41.0
22	36.16113819115035	38.0	34.0	40.0	30.0	41.0
23	36.09728469698006	38.0	34.0	40.0	30.0	41.0
24	36.001075895921446	38.0	34.0	40.0	30.0	41.0
25	35.83551234936278	38.0	34.0	40.0	29.0	41.0
26	35.58572080604035	38.0	34.0	40.0	29.0	41.0
27	35.653523772906645	38.0	34.0	40.0	29.0	41.0
28	35.692457701280105	38.0	34.0	40.0	29.0	41.0
29	35.71205941044796	38.0	34.0	40.0	29.0	41.0
30	35.63976003009796	38.0	34.0	40.0	29.0	41.0
31	35.5714741041965	38.0	34.0	40.0	29.0	41.0
32	35.451485320168224	38.0	34.0	40.0	28.0	41.0
33	35.30799135037469	38.0	34.0	40.0	27.0	41.0
34	35.1740026512623	38.0	34.0	40.0	26.0	41.0
35	35.03850787476796	38.0	34.0	40.0	26.0	41.0
36	34.96257273870206	38.0	34.0	40.0	25.0	41.0
37	34.8933866455635	38.0	33.0	40.0	25.0	41.0
38	34.84823469332396	38.0	33.0	40.0	25.0	41.0
39	34.83478761596324	37.0	33.0	40.0	25.0	41.0
40	34.648306164184845	37.0	33.0	40.0	24.0	41.0
41	34.6018987543313	37.0	33.0	40.0	24.0	41.0
42	34.57732121080602	37.0	33.0	40.0	24.0	41.0
43	34.539646573054775	37.0	33.0	40.0	24.0	41.0
44	34.39906451011796	37.0	33.0	40.0	24.0	41.0
45	34.30220586674341	37.0	33.0	40.0	23.0	41.0
46	34.26166060068546	37.0	33.0	40.0	23.0	41.0
47	34.209246866958054	37.0	33.0	40.0	23.0	41.0
48	34.12744516439477	37.0	33.0	40.0	23.0	41.0
49	33.986677053136106	36.0	33.0	40.0	23.0	41.0
50	33.863513064071384	36.0	33.0	40.0	23.0	41.0
51	33.54417070213634	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	11.0
14	33.0
15	97.0
16	310.0
17	847.0
18	1908.0
19	3542.0
20	6141.0
21	9568.0
22	14638.0
23	21680.0
24	31360.0
25	44801.0
26	58406.0
27	68290.0
28	78545.0
29	92618.0
30	110644.0
31	133826.0
32	162251.0
33	201558.0
34	285106.0
35	335556.0
36	293681.0
37	355238.0
38	479963.0
39	600901.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.26319203489099	26.191092560661776	30.06871109496661	13.477004309480623
2	29.32743679074606	27.63301128201741	29.38729068826675	13.652261238969782
3	27.225709932090886	27.19475101958019	32.143724834826834	13.435814213502095
4	24.35337151402645	29.672466499508193	31.237011999084796	14.737149987380556
5	22.560260786085117	33.85262732073905	29.794857400300508	13.792254492875323
6	21.378780230640952	41.44095162389816	26.874665348898098	10.305602796562795
7	91.1642968847668	2.5632505324932953	5.183642372077774	1.0888102106621316
8	91.94133610410687	1.8598640402501243	4.7428169426039455	1.4559829130390685
9	86.86578456370931	5.249127843207556	6.085608174568168	1.799479418514963
10	39.46488846535786	41.650174903113346	10.3675206215842	8.517416009944592
11	27.656510570846965	25.967097457477195	29.78760416936943	16.58878780230641
12	28.688474321203728	24.273998759284726	29.095097523522877	17.94242939598867
13	22.29828941688741	30.519089560300884	29.944639567495145	17.237981455316557
14	17.478812309971246	34.261402904594654	29.96179965042965	18.297985135004446
15	16.27828465216335	28.782029206343218	38.27426765955339	16.665418481940044
16	20.568836110003797	26.63430624910072	36.526091581770444	16.270766059125037
17	20.097611976912315	26.206513047559966	29.337933336321115	24.357941639206604
18	20.777882481147497	28.11275648721898	32.158054388617494	18.95130664301602
19	24.258224456243557	28.55305119247834	28.418011364574514	18.77071298670359
20	25.84337974614871	29.397964141913295	27.251273148421156	17.50738296351684
21	23.307874296200723	28.298008722747316	30.51805759655053	17.87605938450144
22	22.66808625565811	25.743397201078434	29.968787519253492	21.619729024009963
23	19.287314040132188	30.29114351018637	29.230697560319758	21.190844889361692
24	19.616009236960107	28.350815782086997	33.96673302684993	18.06644195410297
25	19.682674095233153	29.721204673203616	31.015110308079507	19.58101092348372
26	19.02413379911263	33.72843785455326	27.843856218554592	19.40357212777952
27	18.68635732128157	32.71655317031058	30.185853722971395	18.411235785436457
28	17.1037966830916	31.239046441906925	33.356577088281846	18.300579786719627
29	18.041497916022916	27.887523027533973	33.174391259326	20.896587797117107
30	19.37609240734145	29.393187623983074	31.650976886370767	19.579743082304713
31	22.246720714048152	29.28312131883788	29.07911682773164	19.39104113938233
32	22.912130940278193	29.76322034018243	28.828732937216508	18.495915782322875
33	22.23357054740075	29.94363708842337	28.637141495793127	19.18565086838275
34	19.154927833300704	30.50310886450965	29.38820471330278	20.95375858888687
35	18.830419460831376	29.88584711840339	29.998095289763626	21.285638131001605
36	22.709217382279473	29.323397389780375	27.97296962606351	19.994415601876643
37	19.01107208650097	31.817565320356927	29.66034829661115	19.511014296530952
38	19.465519437479507	32.15310096261579	27.166475212820412	21.214904387084296
39	19.474128963625343	31.0393467138736	28.29367447499581	21.192849847505244
40	21.3876846035726	29.283327711587955	27.959819459416106	21.36916822542334
41	17.79199856586523	29.102881478668426	29.083215198054486	24.021904757411857
42	20.189869536194212	29.39528103616237	27.92314051926057	22.491708908382847
43	20.43762929031558	29.30499895034544	28.504313018782916	21.753058740556057
44	19.346194943259682	31.380838261205945	28.691924028597782	20.581042766936587
45	18.454873109737253	32.070602831944406	27.498708571078122	21.97581548724021
46	20.20885766920078	31.257356427306114	27.533677399875927	21.00010850361718
47	19.627508261606938	29.795565032586467	28.889943129951952	21.686983575854644
48	20.731444112381443	28.351169598229976	29.49526358123265	21.422122708155932
49	20.077621364833977	28.23314242986774	29.796921327801222	21.892314877497057
50	18.60727941332566	31.630691427506612	28.362108413983755	21.399920745183973
51	18.032888389877083	31.8410351245079	27.746792656663892	22.379283828951124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2537.0
1	2672.5
2	2808.0
3	16146.5
4	29485.0
5	22246.0
6	15007.0
7	14393.5
8	13780.0
9	15331.0
10	16882.0
11	18349.5
12	19817.0
13	20579.0
14	21341.0
15	21093.0
16	20845.0
17	20180.5
18	19516.0
19	19510.0
20	19504.0
21	20775.0
22	22046.0
23	24446.5
24	26847.0
25	31462.0
26	43570.0
27	51063.0
28	58603.0
29	66143.0
30	76641.0
31	87139.0
32	101123.5
33	115108.0
34	123649.5
35	132191.0
36	142784.5
37	153378.0
38	167175.0
39	180972.0
40	204337.5
41	227703.0
42	254449.5
43	281196.0
44	284130.0
45	287064.0
46	293694.0
47	300324.0
48	289905.0
49	279486.0
50	259641.0
51	239796.0
52	213226.5
53	186657.0
54	163980.5
55	141304.0
56	127626.0
57	113948.0
58	104764.0
59	95580.0
60	82899.5
61	70219.0
62	58655.5
63	47092.0
64	38732.0
65	30372.0
66	23168.0
67	15964.0
68	12935.0
69	9906.0
70	7585.0
71	5264.0
72	4239.0
73	3214.0
74	2486.0
75	1524.0
76	1290.0
77	861.0
78	432.0
79	363.0
80	294.0
81	212.5
82	131.0
83	92.0
84	53.0
85	40.5
86	28.0
87	21.5
88	15.0
89	9.5
90	4.0
91	7.5
92	11.0
93	5.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3391592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.38741383617088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43401577962749	16.34725926639308
2	8.269134435698936	3.53710800486449
3	3.073841445772833	1.972245572025522
4	1.742894813567296	1.491040506027186
5	1.096888812082575	1.1729807478137955
6	0.769795673959285	0.9878363188956782
7	0.5573853733216461	0.8344722151821051
8	0.4308178359214765	0.7371263475884946
9	0.3745325278505345	0.720925395142192
>10	4.672750927422274	24.59930963979491
>50	2.010633174906105	30.76565339028909
>100	0.5637579393698964	15.590734186865213
>500	0.002414857139864031	0.34889438398776945
>1k	9.943529399440128E-4	0.3382610650748716
>5k	0.0	0.0
>10k+	1.420504199920018E-4	0.5561529600557391
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18306	0.5397465261151696	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.845403574486554E-5	0.0	0.0	0.048915081766910645	0.0
2	8.845403574486554E-5	0.0	0.0	0.157890453804585	0.0
3	8.845403574486554E-5	0.0	0.0	0.24377932251284942	0.0
4	8.845403574486554E-5	0.0	0.0	0.4024658626391382	0.0
5	8.845403574486554E-5	0.0	0.0	0.739151407362678	0.0
6	8.845403574486554E-5	0.0	0.0	1.2679001483669026	0.0
7	8.845403574486554E-5	0.0	0.0	1.5691745941139146	0.0
8	8.845403574486554E-5	0.0	0.0	2.1578067173174134	0.0
9	8.845403574486554E-5	0.0	0.0	2.417301373514267	0.0
10	8.845403574486554E-5	0.0	0.0	2.74750028894985	0.0
11	8.845403574486554E-5	0.0	0.0	3.1703105798103075	0.0
12	8.845403574486554E-5	0.0	0.0	3.4981507209593605	0.0
13	8.845403574486554E-5	0.0	0.0	3.675972817485122	0.0
14	8.845403574486554E-5	0.0	0.0	3.7791691925207984	0.0
15	8.845403574486554E-5	0.0	0.0	3.88227711352073	0.0
16	8.845403574486554E-5	0.0	0.0	4.066674293370193	0.0
17	8.845403574486554E-5	0.0	0.0	4.307210301239064	0.0
18	8.845403574486554E-5	0.0	0.0	4.5764643860464345	0.0
19	8.845403574486554E-5	0.0	0.0	4.764783028147254	0.0
20	8.845403574486554E-5	0.0	0.0	4.951833829069063	0.0
21	8.845403574486554E-5	0.0	0.0	5.181283597791244	0.0
22	8.845403574486554E-5	0.0	0.0	5.4595894789231725	0.0
23	8.845403574486554E-5	0.0	0.0	5.745413953093415	0.0
24	8.845403574486554E-5	0.0	0.0	5.967639975563098	0.0
25	8.845403574486554E-5	0.0	0.0	6.156961096735692	0.0
26	8.845403574486554E-5	0.0	0.0	6.341830031442461	0.0
27	8.845403574486554E-5	0.0	0.0	6.541854090940184	0.0
28	8.845403574486554E-5	0.0	0.0	6.7478045708328125	0.0
29	8.845403574486554E-5	0.0	0.0	6.978758058162656	0.0
30	8.845403574486554E-5	0.0	0.0	7.245535429969171	0.0
31	8.845403574486554E-5	0.0	0.0	7.490169808160887	0.0
32	8.845403574486554E-5	0.0	0.0	7.720622055954844	0.0
33	8.845403574486554E-5	0.0	0.0	7.94762459635475	0.0
34	8.845403574486554E-5	0.0	0.0	8.178637053041758	0.0
35	1.1793871432648739E-4	0.0	0.0	8.443586374776212	0.0
36	1.1793871432648739E-4	0.0	0.0	8.693586964469782	0.0
37	1.1793871432648739E-4	0.0	0.0	8.955882665131892	0.0
38	1.1793871432648739E-4	0.0	0.0	9.231151624369913	0.0
39	1.1793871432648739E-4	0.0	0.0	9.560377545412303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10240	0.0	42.033695	1
TATAGCG	290	0.0	41.89655	1
TAACGCG	70	0.0	41.785717	1
CGACCCG	175	0.0	39.857143	32
AGGGCGA	2565	0.0	39.385963	6
TAATGCG	200	0.0	39.375004	1
TACGGGA	1725	0.0	39.0	4
ACGGGAT	2250	0.0	38.8	5
CGTTCGA	35	6.251894E-6	38.57143	14
ACGGGTC	70	0.0	38.57143	5
GGGCGAT	5675	0.0	38.220264	7
TAAACGG	330	0.0	38.181816	2
CGTTAGG	520	0.0	38.07692	2
GCGTAAG	280	0.0	37.76786	1
TAGGGAT	7550	0.0	37.728474	5
TAGGGAC	5060	0.0	37.707508	5
ATAGGGA	6380	0.0	37.69984	4
TAAGGGA	6190	0.0	37.657513	4
TTAGGGA	5775	0.0	37.51948	4
CCGCTCG	30	1.1403914E-4	37.500004	19
>>END_MODULE