FastQCFastQC Report
Thu 26 May 2016
SRR1548008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548008_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2117398
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68110.321668387331999No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35690.16855593516192988No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTA253.8919236E-545.037
TATAGCG1600.045.01
TCGGTAA207.0346345E-445.023
TACCGGT253.8919236E-545.020
CGGTCTA2400.042.18750431
TCGGTAC700.041.78571736
CGACGGT2700.041.66666428
TCGATAG650.041.538461
TACGGGA8250.040.6363644
GACCGAT10200.040.5882349
CGTTTTT40850.039.767441
TAGTAGG12800.039.550782
TATTGCG2050.039.5121961
CGTTAGG1600.039.3752
TAAGGGA26700.039.2696654
GGACCGA10950.039.0410968
TATGGGA25900.039.005794
ACGGTCT2600.038.94230730
ACGGGAT9650.038.9378245
CGCGTAG356.2507825E-638.5714344
Produced by FastQC (version 0.11.5)