##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548008_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2117398
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85077061563296	31.0	31.0	31.0	30.0	33.0
2	31.18941880553396	31.0	31.0	33.0	30.0	34.0
3	31.38375827312579	31.0	31.0	34.0	30.0	34.0
4	35.2466876798788	37.0	35.0	37.0	33.0	37.0
5	35.05687830063125	35.0	35.0	37.0	32.0	37.0
6	35.077601376784145	37.0	35.0	37.0	32.0	37.0
7	35.49769197855103	35.0	35.0	37.0	35.0	37.0
8	35.5933934007683	37.0	35.0	37.0	35.0	37.0
9	37.427883184927914	39.0	37.0	39.0	35.0	39.0
10	36.55147922119507	38.0	35.0	39.0	32.0	39.0
11	36.09541522189026	38.0	35.0	39.0	31.0	39.0
12	35.31070823718545	37.0	35.0	39.0	30.0	39.0
13	34.8290897601679	37.0	33.0	39.0	27.0	39.0
14	35.74683314143113	38.0	34.0	40.0	27.0	41.0
15	36.1070521460774	38.0	34.0	40.0	30.0	41.0
16	36.331993796159246	38.0	34.0	40.0	31.0	41.0
17	36.29232482509193	38.0	34.0	40.0	31.0	41.0
18	36.22532561190669	38.0	35.0	40.0	30.0	41.0
19	36.16145287754121	37.0	34.0	40.0	30.0	41.0
20	36.031509900358834	37.0	34.0	40.0	30.0	41.0
21	35.86486905154345	37.0	34.0	40.0	30.0	41.0
22	35.745691173789716	37.0	34.0	40.0	29.0	41.0
23	35.716076524111195	37.0	34.0	40.0	29.0	41.0
24	35.58172200030415	37.0	34.0	40.0	29.0	41.0
25	35.434206039677	37.0	34.0	40.0	29.0	41.0
26	35.1601271938483	36.0	34.0	40.0	27.0	41.0
27	35.23611904800137	37.0	34.0	40.0	28.0	41.0
28	35.32029358675128	37.0	34.0	40.0	29.0	41.0
29	35.35511698792575	37.0	34.0	40.0	29.0	41.0
30	35.30113375000826	36.0	34.0	40.0	29.0	41.0
31	35.189694143472316	36.0	34.0	40.0	28.0	41.0
32	35.05017856822383	36.0	34.0	40.0	27.0	41.0
33	34.93001079626976	36.0	33.0	40.0	27.0	41.0
34	34.813720897063284	36.0	34.0	40.0	26.0	41.0
35	34.700816284893065	36.0	33.0	40.0	25.0	41.0
36	34.56126198286765	36.0	33.0	40.0	25.0	41.0
37	34.47041415926529	36.0	33.0	40.0	24.0	41.0
38	34.41585332563835	36.0	33.0	40.0	24.0	41.0
39	34.40442514822438	36.0	33.0	40.0	24.0	41.0
40	34.21108360355493	36.0	33.0	40.0	23.0	41.0
41	34.18624132071533	36.0	33.0	40.0	23.0	41.0
42	34.13381943309666	36.0	33.0	40.0	23.0	41.0
43	34.088045799608764	35.0	33.0	40.0	23.0	41.0
44	33.933601051857046	35.0	33.0	40.0	23.0	41.0
45	33.80743865820219	35.0	33.0	40.0	23.0	41.0
46	33.775357301744876	35.0	33.0	40.0	23.0	41.0
47	33.72250233541356	35.0	33.0	40.0	23.0	41.0
48	33.64164129747927	35.0	32.0	39.0	23.0	41.0
49	33.51988100489374	35.0	32.0	39.0	23.0	41.0
50	33.363447495463774	35.0	32.0	39.0	23.0	41.0
51	33.01541325721475	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	17.0
15	90.0
16	268.0
17	649.0
18	1455.0
19	2614.0
20	4517.0
21	7058.0
22	10443.0
23	15177.0
24	21315.0
25	29099.0
26	37174.0
27	44270.0
28	52143.0
29	62159.0
30	73794.0
31	89585.0
32	109227.0
33	138290.0
34	213512.0
35	251205.0
36	166969.0
37	198548.0
38	264912.0
39	322868.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.62310628422242	24.328066806523857	30.13788621695118	12.910940692302534
2	28.24962524759162	27.32145775144777	30.67453544397416	13.754381556986454
3	27.956387981853197	26.69224208202709	31.415775399806744	13.935594536312964
4	24.527981985436842	29.48760695910736	30.729886398305844	15.254524657149954
5	22.421056409801086	34.10265807373011	28.954830409776527	14.521455106692272
6	20.66304964867257	41.960793388866904	26.857917122808278	10.518239839652251
7	90.91573714530759	3.3803753474783678	4.477807195435152	1.226080311778891
8	91.61456655763347	2.3633724032987655	4.049167893801732	1.9728931452660292
9	87.33502156892563	5.039345460796694	5.569996760174516	2.055636210103155
10	46.87914128567232	33.10124029587257	10.169226569591546	9.85039184886356
11	38.989363360124074	23.017968279936035	22.864666916659033	15.128001443280855
12	36.6070998461319	23.307002273545173	23.76204190237263	16.323855977950295
13	22.73512112507899	36.74042385985063	24.8397325396548	15.68472247541558
14	16.918737053685703	38.8762528348473	27.594528756520976	16.610481354946025
15	15.806522911611326	26.36717329477028	42.119337035361326	15.706966758257067
16	19.038555812369708	23.384691966271813	40.29417237571774	17.282579845640736
17	19.424076153845427	22.937822742819254	26.76563404707098	30.87246705626434
18	21.84053257819267	25.368258589079616	32.31598405212435	20.475224780603362
19	27.23658943665763	27.009046008355536	25.414352899171533	20.3400116558153
20	29.470416048376357	27.656774966255753	24.36646298900821	18.506345996359684
21	23.384928105155478	29.197864548847217	28.641521338926363	18.775686007070945
22	23.59764201156325	24.357678622535772	26.655215505068014	25.38946386083297
23	19.97031261954531	30.529121119411656	26.185251898792767	23.315314362250273
24	20.06009262311573	26.422099199111358	35.596850474025196	17.920957703747714
25	19.10231331095996	27.460260187267583	32.38531442836916	21.0521120734033
26	17.976261430302664	35.102422879401985	26.062223540401945	20.859092149893407
27	18.303738834172886	34.03573631409872	28.79255576892016	18.86796908280824
28	15.985563413208098	30.68539783262287	34.69295805512237	18.63608069904666
29	16.14864092626894	27.7609594417299	34.327981796525734	21.76241783547543
30	19.126777299307925	31.318344496405494	29.947085998947763	19.607792205338818
31	26.344315050831256	28.057786018500064	25.14288763850726	20.45501129216142
32	26.85480008954386	27.953459859695723	26.33704197321429	18.85469807754612
33	25.4402337208215	28.335485345693158	25.04408712958074	21.180193803904604
34	18.756653213047333	29.148133699946822	28.450201615378873	23.64501147162697
35	18.75764499635874	28.538139735656685	29.77640481383283	22.927810454151746
36	27.129665750132947	26.876241500180882	26.76218641936943	19.231906330316736
37	19.603683388763002	32.02019648644232	28.500924247590675	19.875195877204003
38	19.976263319413732	32.39452384483219	25.17641935998806	22.45279347576601
39	19.673155448338004	31.170899377443444	27.735739808954197	21.420205365264348
40	22.90769142126327	26.850407906307648	26.946091381969755	23.295809290459328
41	17.280737962348127	25.63164789992245	29.13675180575404	27.95086233197538
42	20.966251975301763	27.134577438913233	26.137079566524573	25.76209101926043
43	21.391443649233636	26.46743786477554	27.611672439475242	24.52944604651558
44	19.375762138247037	30.33312584596755	28.170282582679306	22.120829433106103
45	17.900177481984965	34.525488358825314	24.26341198017567	23.310922179014053
46	21.565808600933785	31.550846841264608	26.13311243327896	20.750232124522643
47	20.279654557149858	28.178122393617073	27.83874359001	23.703479459223065
48	21.76865190200425	26.09995853401203	30.535213502610283	21.59617606137344
49	21.100567772331893	25.386205144238357	30.532332608229535	22.980894475200223
50	19.01361954625441	31.852254512377925	27.701641354152596	21.432484587215065
51	18.203238125283956	32.397026916999074	25.683126176561988	23.71660878115498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2045.0
1	2328.5
2	2612.0
3	7076.0
4	11540.0
5	8854.5
6	6169.0
7	6313.5
8	6458.0
9	7275.0
10	8092.0
11	8863.0
12	9634.0
13	10012.5
14	10391.0
15	10323.5
16	10256.0
17	10152.0
18	10048.0
19	10274.5
20	10501.0
21	10584.0
22	10667.0
23	11392.5
24	12118.0
25	15539.0
26	20464.5
27	21969.0
28	25668.5
29	29368.0
30	32890.0
31	36412.0
32	42466.5
33	48521.0
34	57365.5
35	66210.0
36	69734.5
37	73259.0
38	80002.5
39	86746.0
40	115064.0
41	143382.0
42	165955.0
43	188528.0
44	194927.0
45	201326.0
46	198513.0
47	195700.0
48	189977.0
49	184254.0
50	170531.0
51	156808.0
52	143397.0
53	129986.0
54	117905.5
55	105825.0
56	95050.5
57	84276.0
58	75907.5
59	67539.0
60	61077.5
61	54616.0
62	47948.0
63	41280.0
64	34675.5
65	28071.0
66	22287.5
67	16504.0
68	13251.5
69	9999.0
70	8271.5
71	6544.0
72	5543.5
73	4543.0
74	3848.0
75	2268.5
76	1384.0
77	1089.5
78	795.0
79	599.5
80	404.0
81	317.5
82	231.0
83	172.0
84	113.0
85	92.5
86	72.0
87	56.5
88	41.0
89	34.5
90	28.0
91	20.0
92	12.0
93	9.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2117398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.196113790425585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69259420257322	15.892845869789534
2	8.222970971217745	3.3214411486015996
3	2.8598972411756964	1.7327643033512568
4	1.4305431051364856	1.1556564533378086
5	0.8537431994857662	0.8621147402308271
6	0.5988408012220677	0.7256554178298305
7	0.41698215772980496	0.589499337426183
8	0.3247467953163769	0.5246898585028476
9	0.2719954698704338	0.4943926313985199
>10	3.3644244829785577	17.0806433257049
>50	1.9493852714507875	28.80257135691091
>100	1.008615622962217	27.57866425044163
>500	0.004543313578923588	0.6682344505469526
>1k	4.782435346235355E-4	0.2419011608578222
>5k	2.3912176731176774E-4	0.3289256950694005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6811	0.321668387331999	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3569	0.16855593516192988	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049872532230596234	0.0
2	0.0	0.0	0.0	0.21233608419390212	0.0
3	0.0	0.0	0.0	0.3140647152779024	0.0
4	0.0	0.0	0.0	0.5790125427529449	0.0
5	0.0	0.0	0.0	1.0079824388234995	0.0
6	0.0	0.0	0.0	1.5639005987537535	0.0
7	0.0	0.0	0.0	1.8359325927388237	0.0
8	0.0	0.0	0.0	2.457544590105403	0.0
9	0.0	0.0	0.0	2.6510840191593643	0.0
10	0.0	0.0	0.0	2.974405378677037	0.0
11	0.0	0.0	0.0	3.479695361948958	0.0
12	0.0	0.0	0.0	3.8682382811356204	0.0
13	0.0	0.0	0.0	4.041989271738237	0.0
14	0.0	0.0	0.0	4.119678964464876	0.0
15	0.0	0.0	0.0	4.216259767884923	0.0
16	0.0	0.0	0.0	4.412160585775561	0.0
17	0.0	0.0	0.0	4.657178291469058	0.0
18	0.0	0.0	0.0	4.929399196561063	0.0
19	0.0	0.0	0.0	5.087942843055486	0.0
20	0.0	0.0	0.0	5.241433117439423	0.0
21	0.0	0.0	0.0	5.464112084737966	0.0
22	0.0	0.0	0.0	5.720700595731175	0.0
23	0.0	0.0	0.0	5.993771600804384	0.0
24	0.0	0.0	0.0	6.212530662634045	0.0
25	0.0	0.0	0.0	6.390768292026346	0.0
26	0.0	0.0	0.0	6.564708193735896	0.0
27	0.0	0.0	0.0	6.736617301045906	0.0
28	0.0	0.0	0.0	6.917783052595686	0.0
29	0.0	0.0	0.0	7.118642787043343	0.0
30	0.0	0.0	0.0	7.3589377150634885	0.0
31	0.0	0.0	0.0	7.5699514215088515	0.0
32	0.0	0.0	0.0	7.761271145056338	0.0
33	0.0	0.0	0.0	7.9553300796543684	0.0
34	0.0	0.0	0.0	8.159542986250106	0.0
35	0.0	0.0	0.0	8.400546330921253	0.0
36	0.0	0.0	0.0	8.599894776513437	0.0
37	0.0	0.0	0.0	8.817189777264359	0.0
38	0.0	0.0	0.0	9.055076088671095	0.0
39	0.0	0.0	0.0	9.380333787034841	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCGTA	25	3.8919236E-5	45.0	37
TATAGCG	160	0.0	45.0	1
TCGGTAA	20	7.0346345E-4	45.0	23
TACCGGT	25	3.8919236E-5	45.0	20
CGGTCTA	240	0.0	42.187504	31
TCGGTAC	70	0.0	41.785717	36
CGACGGT	270	0.0	41.666664	28
TCGATAG	65	0.0	41.53846	1
TACGGGA	825	0.0	40.636364	4
GACCGAT	1020	0.0	40.588234	9
CGTTTTT	4085	0.0	39.76744	1
TAGTAGG	1280	0.0	39.55078	2
TATTGCG	205	0.0	39.512196	1
CGTTAGG	160	0.0	39.375	2
TAAGGGA	2670	0.0	39.269665	4
GGACCGA	1095	0.0	39.041096	8
TATGGGA	2590	0.0	39.00579	4
ACGGTCT	260	0.0	38.942307	30
ACGGGAT	965	0.0	38.937824	5
CGCGTAG	35	6.2507825E-6	38.57143	44
>>END_MODULE