FastQCFastQC Report
Thu 26 May 2016
SRR1548007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548007_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2327289
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169190.7269831980471698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95250.041.881891
CGTTAGG2950.039.661022
TTAGGGA40100.039.276814
AGGGCGA19400.039.201036
TAGGGAT48050.039.0998955
TACGGGA12000.039.04
TAAGGGA40050.038.8764084
GGGCGAT38150.038.512457
TACGCGG1950.038.0769232
GTAGGGA40850.038.0599754
AGGGATG61350.037.9584356
GGCGATT11400.037.58
AGGGATC31700.037.476346
TAACGGG7350.037.346943
AAGGGAT50800.037.293315
GCGATCG1700.037.0588239
TAGGGAC30650.036.9249575
AGGGATT48350.036.902796
ATAGCGG4100.036.768292
TATGGGA32800.036.2195134
Produced by FastQC (version 0.11.5)