##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548007_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2327289
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83658926759848	31.0	31.0	31.0	30.0	33.0
2	31.16709098010604	31.0	31.0	33.0	30.0	34.0
3	31.2677097687481	31.0	31.0	34.0	28.0	34.0
4	35.202756941660446	35.0	35.0	37.0	33.0	37.0
5	35.05563597817031	35.0	35.0	37.0	32.0	37.0
6	35.09576206478869	37.0	35.0	37.0	32.0	37.0
7	35.504196083941444	35.0	35.0	37.0	35.0	37.0
8	35.59122824883373	37.0	35.0	37.0	35.0	37.0
9	37.40319229799135	39.0	37.0	39.0	35.0	39.0
10	36.53474536252266	38.0	35.0	39.0	32.0	39.0
11	36.15847967313041	38.0	35.0	39.0	31.0	39.0
12	35.82161175513656	37.0	35.0	39.0	30.0	39.0
13	35.671842646100245	37.0	35.0	39.0	30.0	39.0
14	36.720321799312416	39.0	35.0	40.0	31.0	41.0
15	36.8800733385497	39.0	35.0	40.0	31.0	41.0
16	36.94842582936627	39.0	35.0	41.0	31.0	41.0
17	36.8824671108745	38.0	35.0	40.0	31.0	41.0
18	36.76463258323311	38.0	35.0	40.0	31.0	41.0
19	36.687144140671826	38.0	35.0	40.0	31.0	41.0
20	36.52392891471579	38.0	35.0	40.0	30.0	41.0
21	36.41956069916542	38.0	35.0	40.0	30.0	41.0
22	36.29916138477	38.0	35.0	40.0	30.0	41.0
23	36.19949434728562	38.0	34.0	40.0	30.0	41.0
24	36.08242508773083	38.0	34.0	40.0	30.0	41.0
25	35.91474458049688	38.0	34.0	40.0	29.0	41.0
26	35.68395803013721	38.0	34.0	40.0	29.0	41.0
27	35.75194528913255	38.0	34.0	40.0	29.0	41.0
28	35.75239302037693	38.0	34.0	40.0	29.0	41.0
29	35.72858119468618	38.0	34.0	40.0	29.0	41.0
30	35.65504069327015	38.0	34.0	40.0	29.0	41.0
31	35.58336631161837	38.0	34.0	40.0	28.0	41.0
32	35.435007856781	38.0	34.0	40.0	27.0	41.0
33	35.25198503494839	38.0	34.0	40.0	27.0	41.0
34	35.05048105327701	38.0	34.0	40.0	25.0	41.0
35	34.8775154267476	38.0	33.0	40.0	25.0	41.0
36	34.78813675482503	38.0	33.0	40.0	24.0	41.0
37	34.72385036839	38.0	33.0	40.0	24.0	41.0
38	34.65335804878552	38.0	33.0	40.0	24.0	41.0
39	34.60196950185387	38.0	33.0	40.0	24.0	41.0
40	34.42799884328934	37.0	33.0	40.0	23.0	41.0
41	34.32923629166812	37.0	33.0	40.0	23.0	41.0
42	34.301970232317515	37.0	33.0	40.0	23.0	41.0
43	34.263287885604235	37.0	33.0	40.0	23.0	41.0
44	34.11088309187213	37.0	33.0	40.0	23.0	41.0
45	34.009159154707476	37.0	33.0	40.0	23.0	41.0
46	33.949957654592964	37.0	33.0	40.0	23.0	41.0
47	33.901197487720694	37.0	33.0	40.0	23.0	41.0
48	33.800595456773955	36.0	32.0	40.0	22.0	41.0
49	33.6541160122357	36.0	32.0	40.0	22.0	41.0
50	33.55972764877933	36.0	32.0	40.0	22.0	41.0
51	33.21898268758199	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	8.0
14	37.0
15	117.0
16	297.0
17	739.0
18	1534.0
19	2838.0
20	4788.0
21	7621.0
22	11186.0
23	16360.0
24	23412.0
25	33085.0
26	43464.0
27	50735.0
28	55724.0
29	63566.0
30	74496.0
31	89063.0
32	107722.0
33	132215.0
34	181622.0
35	211049.0
36	208784.0
37	253913.0
38	342069.0
39	410799.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.214215338103692	24.439293959624266	29.12814008058303	15.21835062168901
2	31.20128183478717	26.51651771653628	29.1610968813929	13.121103567283651
3	28.045034372611223	26.26936319468704	32.26720016293636	13.418402269765378
4	24.732983312343247	29.038593831707193	31.548166128057154	14.68025672789241
5	23.041229516402993	32.83786414149682	30.480829841072595	13.64007650102759
6	21.339507040165618	42.088498678075645	26.518107549169873	10.053886732588863
7	90.03192126117555	2.6879772989087303	6.223593202219407	1.0565082376963066
8	90.4836485713635	2.334175085260146	5.9939698077892345	1.1882065355871143
9	85.10859631098673	5.596597586290315	7.280874872007731	2.013931230715223
10	37.00902638219834	41.2191180381981	12.489596264151121	9.282259315452443
11	28.880555874238222	25.61344981220639	28.472828256396177	17.03316605715921
12	29.64539427634471	22.895093819461184	29.000910501446103	18.458601402748005
13	24.269396710077693	26.869589466542404	29.430165312515978	19.430848510863928
14	19.289869027868907	29.859506060484968	29.818814938754922	21.031809972891207
15	18.92068410927908	27.8101258588856	34.71519867107179	18.553991360763533
16	22.467514777924013	26.16151238630011	33.28920473563876	18.081768100137115
17	22.548037652392978	26.210453450345018	29.056210896025377	22.18529800123663
18	22.696794424757734	26.884714360786305	30.455263613586453	19.96322760086951
19	25.41338871107112	27.99089412616998	27.061658436060153	19.534058726698746
20	27.101189409652175	27.066728713107825	28.170115529270323	17.661966347969678
21	25.54727839988931	26.518408328316767	29.393212445897348	18.54110082589657
22	24.61344508567694	24.737580936445795	29.033867302255974	21.61510667562129
23	22.353089796754936	27.24835634938334	29.495950008787048	20.902603845074676
24	21.652747037432825	26.614872497571206	32.078525700933575	19.65385476406239
25	21.64686035984358	28.777861279798085	29.17132337238736	20.40395498797098
26	21.747105752658996	30.908151071912428	27.401796682749758	19.94294649267882
27	20.59722707407632	30.4148732710033	29.34444325565067	19.643456399269706
28	19.887087508255313	29.237709626952217	31.495014155955708	19.380188708836762
29	20.573508489921107	27.223563553989212	31.297703035592058	20.905224920497627
30	21.3098588099716	27.934003898957112	30.253311900670692	20.50282539040059
31	23.133568714500004	28.28376707834738	28.25317354226312	20.32949066488949
32	24.193170680564382	28.284927226485408	27.90087522434902	19.621026868601195
33	23.996074402448514	28.12375257219881	27.763762901814086	20.11641012353859
34	21.541200942384037	28.840981932196648	28.619135827136212	20.99868129828311
35	21.86741741141732	28.052897598880072	28.98032861410852	21.09935637559409
36	23.289200438793806	29.186620140429486	26.77677761550027	20.74740180527644
37	21.51851360101818	30.31651848996837	28.173724879033074	19.991243029980378
38	21.739285494839706	29.792389342277648	27.404675568870047	21.063649594012603
39	21.481174018353542	29.372673527009326	27.88592220390334	21.260230250733795
40	23.31923538503383	27.95346860660623	28.232247907329082	20.495048101030854
41	20.228085123936047	28.21170898844106	29.47261814067784	22.08758774694505
42	21.660438389903447	27.94646474932851	27.939331986702125	22.45376487406592
43	21.917905339646257	28.70223680857856	27.925453177495363	21.454404674279818
44	21.117188282160058	29.108245688438352	28.450312788828548	21.324253240573043
45	20.679425718077987	30.00302068200382	26.838222498366125	22.479331101552063
46	20.993611021235438	30.189847500675675	27.082197354948185	21.7343441231407
47	21.06545426889398	28.83569681290119	28.631424803709383	21.467424114495447
48	21.502443400884033	28.088131727516437	29.053718725951093	21.355706145648433
49	20.8446394066229	28.34903615322377	28.80252516984354	22.00379927030979
50	20.16315979665611	29.500977317385164	28.89654013747326	21.43932274848547
51	19.70438566074089	30.10713323527933	27.88184020119547	22.306640902784313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1551.0
1	1927.5
2	2304.0
3	15203.0
4	28102.0
5	21113.5
6	14125.0
7	13272.5
8	12420.0
9	12929.0
10	13438.0
11	13922.0
12	14406.0
13	14579.0
14	14752.0
15	14055.5
16	13359.0
17	12813.0
18	12267.0
19	12063.5
20	11860.0
21	12213.5
22	12567.0
23	13207.0
24	13847.0
25	17460.5
26	25579.5
27	30085.0
28	35470.5
29	40856.0
30	46235.0
31	51614.0
32	59255.0
33	66896.0
34	74953.0
35	83010.0
36	90821.0
37	98632.0
38	105207.5
39	111783.0
40	122020.5
41	132258.0
42	143409.5
43	154561.0
44	157464.5
45	160368.0
46	162903.0
47	165438.0
48	163747.5
49	162057.0
50	157617.5
51	153178.0
52	142258.5
53	131339.0
54	123943.0
55	116547.0
56	111214.5
57	105882.0
58	101290.5
59	96699.0
60	90707.5
61	84716.0
62	74260.0
63	63804.0
64	53905.5
65	44007.0
66	37308.0
67	30609.0
68	25597.5
69	20586.0
70	17149.0
71	13712.0
72	11540.0
73	9368.0
74	7681.0
75	4759.5
76	3525.0
77	2611.0
78	1697.0
79	1360.0
80	1023.0
81	792.5
82	562.0
83	375.0
84	188.0
85	151.5
86	115.0
87	89.5
88	64.0
89	47.5
90	31.0
91	20.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2327289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.8528420946391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69926097166594	17.57936834448746
2	6.446069788304099	3.0751416958288273
3	2.7088040608071062	1.9383802658324727
4	1.65742509404928	1.5813719620819935
5	1.2357538848821241	1.4738121141965064
6	0.9423685017830575	1.3486900240795738
7	0.8276334541107248	1.3818987065200699
8	0.6936011598777628	1.3235487154578245
9	0.6288588776307262	1.3500064357143915
>10	10.606977037232264	59.040139477237844
>50	0.5170483899740805	7.47475605079784
>100	0.035090718710080175	1.4169896018196098
>500	1.8467682880021465E-4	0.023390907022683264
>1k	7.387073152008586E-4	0.24721237327928153
>5k	0.0	0.0
>10k+	1.8467682880021465E-4	0.74529332564365
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16919	0.7269831980471698	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016757695327052204	0.0
2	0.0	0.0	0.0	0.05315197210144507	0.0
3	0.0	0.0	0.0	0.08563611996619243	0.0
4	0.0	0.0	0.0	0.14304196857373536	0.0
5	0.0	0.0	0.0	0.266361418801017	0.0
6	0.0	0.0	0.0	0.521508072267776	0.0
7	0.0	0.0	0.0	0.669577349439627	0.0
8	0.0	0.0	0.0	0.9517081892278957	0.0
9	0.0	0.0	0.0	1.1198437323426527	0.0
10	0.0	0.0	0.0	1.3263501009114038	0.0
11	0.0	0.0	0.0	1.5315674159934585	0.0
12	0.0	0.0	0.0	1.6860389921492345	0.0
13	0.0	0.0	0.0	1.7855539213221907	0.0
14	0.0	0.0	0.0	1.8554206203011314	0.0
15	0.0	0.0	0.0	1.9205607898288524	0.0
16	0.0	0.0	0.0	2.015993716293937	0.0
17	0.0	0.0	0.0	2.1249187359197763	0.0
18	0.0	0.0	0.0	2.2514608198637984	0.0
19	0.0	0.0	0.0	2.3480968629164662	0.0
20	0.0	0.0	0.0	2.447568823639866	0.0
21	0.0	0.0	0.0	2.57011484177513	0.0
22	0.0	0.0	0.0	2.7105787033754725	0.0
23	0.0	0.0	0.0	2.8598510971349067	0.0
24	0.0	0.0	0.0	2.982440083719727	0.0
25	0.0	0.0	0.0	3.102064247285146	0.0
26	0.0	0.0	0.0	3.213352531636595	0.0
27	0.0	0.0	0.0	3.334480590936493	0.0
28	0.0	0.0	0.0	3.46643669952464	0.0
29	0.0	0.0	0.0	3.6026896530684414	0.0
30	0.0	0.0	0.0	3.7724579972663475	0.0
31	0.0	0.0	0.0	3.9313123552768907	0.0
32	0.0	0.0	0.0	4.090682334682113	0.0
33	0.0	0.0	0.0	4.243950794250305	0.0
34	0.0	0.0	0.0	4.405039511637789	0.0
35	0.0	0.0	0.0	4.582671082104543	0.0
36	0.0	0.0	0.0	4.7527831738989015	0.0
37	0.0	0.0	0.0	4.93445377862397	0.0
38	0.0	0.0	0.0	5.121280597295824	0.0
39	0.0	0.0	0.0	5.389962312372894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9525	0.0	41.88189	1
CGTTAGG	295	0.0	39.66102	2
TTAGGGA	4010	0.0	39.27681	4
AGGGCGA	1940	0.0	39.20103	6
TAGGGAT	4805	0.0	39.099895	5
TACGGGA	1200	0.0	39.0	4
TAAGGGA	4005	0.0	38.876408	4
GGGCGAT	3815	0.0	38.51245	7
TACGCGG	195	0.0	38.076923	2
GTAGGGA	4085	0.0	38.059975	4
AGGGATG	6135	0.0	37.958435	6
GGCGATT	1140	0.0	37.5	8
AGGGATC	3170	0.0	37.47634	6
TAACGGG	735	0.0	37.34694	3
AAGGGAT	5080	0.0	37.29331	5
GCGATCG	170	0.0	37.058823	9
TAGGGAC	3065	0.0	36.924957	5
AGGGATT	4835	0.0	36.90279	6
ATAGCGG	410	0.0	36.76829	2
TATGGGA	3280	0.0	36.219513	4
>>END_MODULE