FastQCFastQC Report
Thu 26 May 2016
SRR1548006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548006_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3333086
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81480.24445813879389852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG1850.043.7837832
CGTTTTT66100.041.0514371
CTACGCG451.9301297E-840.01
AGGGCGA29200.038.9897236
GTAGGGT31400.038.9808924
CGTAAGG5600.038.5714262
GGGCGAT54800.038.553837
TACGGGT7950.038.2075464
CGTTAGG5550.038.108112
TAGGGTC29700.037.8030325
CGAGGGT14650.037.781574
GGCGATA9600.037.734378
TAGGGTA24500.037.6530655
GACCGAT18050.037.6454289
TATGGGC17050.036.818184
TAAGGGA39200.036.7346954
ATAGGGT31100.036.607724
TAGGGCG13050.036.379315
AGGGTAC20250.036.3333326
GCGATGT7950.036.2264149
Produced by FastQC (version 0.11.5)