##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548006_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3333086
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.774531770257354	31.0	31.0	31.0	30.0	33.0
2	31.123372154213843	31.0	31.0	33.0	30.0	34.0
3	31.342823737521325	31.0	31.0	34.0	30.0	34.0
4	35.223639294035614	35.0	35.0	37.0	33.0	37.0
5	35.023485442619844	35.0	35.0	37.0	32.0	37.0
6	35.05671530827588	36.0	35.0	37.0	32.0	37.0
7	35.461067611216755	35.0	35.0	37.0	35.0	37.0
8	35.54929665781201	37.0	35.0	37.0	35.0	37.0
9	37.34965584446366	39.0	37.0	39.0	35.0	39.0
10	36.21703760419023	38.0	35.0	39.0	31.0	39.0
11	36.00238127669073	37.0	35.0	39.0	31.0	39.0
12	35.62562322124302	37.0	35.0	39.0	30.0	39.0
13	35.42597730751622	37.0	35.0	39.0	30.0	39.0
14	36.38661888712142	38.0	35.0	40.0	30.0	41.0
15	36.570738948830005	38.0	35.0	40.0	30.0	41.0
16	36.66875982197879	38.0	35.0	40.0	31.0	41.0
17	36.61090832939804	38.0	35.0	40.0	31.0	41.0
18	36.53114111067041	38.0	35.0	40.0	30.0	41.0
19	36.5066805957002	38.0	35.0	40.0	30.0	41.0
20	36.4007517357788	38.0	35.0	40.0	30.0	41.0
21	36.304265776520616	38.0	34.0	40.0	30.0	41.0
22	36.17163253513411	38.0	34.0	40.0	30.0	41.0
23	36.09252296520402	38.0	34.0	40.0	30.0	41.0
24	35.99688216865692	38.0	34.0	40.0	30.0	41.0
25	35.83325632761951	38.0	34.0	40.0	29.0	41.0
26	35.59733022190247	38.0	34.0	40.0	29.0	41.0
27	35.697035420028165	38.0	34.0	40.0	29.0	41.0
28	35.738956030537466	38.0	34.0	40.0	29.0	41.0
29	35.77179976754275	38.0	34.0	40.0	29.0	41.0
30	35.71023219922918	38.0	34.0	40.0	29.0	41.0
31	35.6378329272032	38.0	34.0	40.0	29.0	41.0
32	35.53470177487169	38.0	34.0	40.0	28.0	41.0
33	35.42767243329455	38.0	34.0	40.0	27.0	41.0
34	35.328497074482925	38.0	34.0	40.0	27.0	41.0
35	35.20277544593809	38.0	34.0	40.0	26.0	41.0
36	35.13022976304842	38.0	34.0	40.0	26.0	41.0
37	35.08829895178222	38.0	34.0	40.0	26.0	41.0
38	35.02330002886214	38.0	33.0	40.0	26.0	41.0
39	35.00773007357146	38.0	34.0	40.0	26.0	41.0
40	34.85224653669302	38.0	33.0	40.0	25.0	41.0
41	34.783662647768466	37.0	33.0	40.0	24.0	41.0
42	34.74507768476421	37.0	33.0	40.0	24.0	41.0
43	34.720694875559765	37.0	33.0	40.0	24.0	41.0
44	34.59348573664166	37.0	33.0	40.0	24.0	41.0
45	34.46504770653983	37.0	33.0	40.0	24.0	41.0
46	34.39262353266612	37.0	33.0	40.0	24.0	41.0
47	34.34401032556616	37.0	33.0	40.0	24.0	41.0
48	34.25471920016465	37.0	33.0	40.0	23.0	41.0
49	34.11933925497272	36.0	33.0	40.0	24.0	41.0
50	34.00614685609672	36.0	33.0	40.0	23.0	41.0
51	33.68204990810318	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	11.0
14	31.0
15	134.0
16	346.0
17	896.0
18	1752.0
19	3393.0
20	5825.0
21	9260.0
22	13831.0
23	20264.0
24	29209.0
25	40495.0
26	52691.0
27	64403.0
28	75469.0
29	90662.0
30	109427.0
31	132157.0
32	160872.0
33	198634.0
34	281131.0
35	321708.0
36	289787.0
37	356728.0
38	493468.0
39	580461.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.297482273184666	25.709267627657972	30.152417309364353	12.840832789793003
2	28.38717632848357	27.378951518202648	30.605540931137092	13.628331222176685
3	28.453871277248773	26.999813386153253	31.168832727388374	13.377482609209604
4	25.06304967828613	29.41466256796254	30.52567500508538	14.996612748665953
5	22.74756786953592	33.547259206633136	29.493268400515316	14.21190452331563
6	20.913531784058378	43.366087763712066	25.45817899688157	10.262201455347988
7	92.62887306238123	2.4024882646292354	3.9722047375915297	0.9964339353980065
8	93.55966212692982	1.552225175107993	3.605067496008204	1.283045201953985
9	88.30990259477252	3.6621617324005444	6.102002768605431	1.9259329042214932
10	34.643480546256534	28.53340717881267	23.860020413514683	12.963091861416117
11	32.82009525106763	23.883932187768334	24.475066049900903	18.820906511263136
12	31.76974731525079	24.82867228748373	24.362617706233802	19.038962691031674
13	24.84643360537352	28.609252806558246	26.614134768799847	19.93017881926839
14	20.36458105191405	31.22373680127065	28.036120280124788	20.375561866690507
15	20.251532663723648	26.760335616902776	34.91629078877653	18.071840930597048
16	23.101594138285062	24.706893251479258	33.87167327815724	18.31983933207844
17	22.849575438497535	24.780548716714783	28.073383045021938	24.296492799765744
18	23.469301422165525	25.96488659458532	30.536265790921686	20.029546192327473
19	25.76477174606356	27.053607377667422	26.857272809642474	20.324348066626545
20	27.136083497395507	27.657042152527715	26.42824097548038	18.778633374596396
21	25.307027781461382	27.238691110880424	28.438900166392344	19.015380941265843
22	24.652949248834265	24.110208977506133	28.55407871264048	22.68276306101913
23	21.852091425183747	27.43082536724225	28.720261043369415	21.996822164204584
24	21.61168358692215	26.52295800348386	32.695405999125136	19.169952410468856
25	21.21025380083202	27.9159913665594	29.93520119192844	20.938553640680137
26	20.995407859263157	31.061694777752507	26.874404080782792	21.06849328220154
27	20.333678758963917	30.364743063935347	28.909485083793218	20.392093093307523
28	18.734290084324257	29.17296463397584	31.72816422978585	20.36458105191405
29	19.827361190200314	26.877974345696448	31.493606825626465	21.801057638476777
30	21.800487596179636	27.901950324714093	29.29918399945276	20.99837807965351
31	24.002260967763807	27.31723693898087	27.37859149148867	21.301910601766654
32	24.669360466546618	26.933538468554367	27.688394478870332	20.708706586028683
33	23.54958737938355	27.594427506520987	27.618099262965313	21.23788585113015
34	20.206349311118885	27.920251682674856	29.247340152639328	22.626058853566935
35	20.831295682139615	27.81347375975297	29.630468580768692	21.72476197733872
36	24.161362773117766	27.67303333907376	27.627279944171857	20.53832394363662
37	21.285259366244976	29.72437554866571	28.647745662728173	20.34261942236114
38	20.99414776576422	29.60733686439534	27.842845939168686	21.555669430671756
39	21.227745098686324	28.839639901280673	28.350963641502197	21.581651358530802
40	23.075612210426012	26.874344076330463	27.973685647475044	22.07635806576848
41	20.00140410418453	26.17115189947094	29.263991388161003	24.56345260818353
42	21.516696538883185	26.72169274960202	28.131497357103896	23.6301133544109
43	21.401397983730394	27.07670909181461	28.26260708544574	23.259285839009255
44	21.064022950502928	28.08412384198908	28.305090237695634	22.54676296981236
45	20.21447991440965	29.849124805060534	26.792167978864036	23.144227301665783
46	21.437760681842594	29.15355319364697	27.55485457020911	21.85383155430133
47	21.06675315308396	27.698505229088	28.48570363921003	22.749037978618013
48	21.60787330419917	27.006653893718912	29.262221256817256	22.123251545264658
49	21.34292364493445	26.446632340119635	29.607156851038347	22.603287163907563
50	20.316247465561947	28.979840304150567	28.614263178327832	22.089649051959658
51	19.855923309509567	29.047735341962373	27.757249587919425	23.33909176060864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1534.0
1	1987.5
2	2441.0
3	9379.5
4	16318.0
5	13008.5
6	9699.0
7	9167.0
8	8635.0
9	9000.5
10	9366.0
11	9466.5
12	9567.0
13	9450.5
14	9334.0
15	9326.0
16	9318.0
17	9264.5
18	9211.0
19	9615.0
20	10019.0
21	11429.0
22	12839.0
23	14523.0
24	16207.0
25	18235.5
26	25625.0
27	30986.0
28	41472.5
29	51959.0
30	59595.5
31	67232.0
32	78266.0
33	89300.0
34	100711.0
35	112122.0
36	122501.5
37	132881.0
38	148760.5
39	164640.0
40	188919.5
41	213199.0
42	233577.0
43	253955.0
44	260210.0
45	266465.0
46	274425.5
47	282386.0
48	282487.5
49	282589.0
50	272433.5
51	262278.0
52	241813.0
53	221348.0
54	201937.5
55	182527.0
56	169699.5
57	156872.0
58	142275.5
59	127679.0
60	114599.0
61	101519.0
62	85269.0
63	69019.0
64	58381.0
65	47743.0
66	38337.5
67	28932.0
68	22603.0
69	16274.0
70	13205.0
71	10136.0
72	8150.5
73	6165.0
74	5244.5
75	3756.5
76	3189.0
77	2313.5
78	1438.0
79	1011.5
80	585.0
81	455.5
82	326.0
83	232.0
84	138.0
85	103.5
86	69.0
87	46.0
88	23.0
89	21.5
90	20.0
91	14.0
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3333086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.352541979425144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84120923635575	15.84266478712147
2	7.131945396518338	2.9030643615521474
3	2.4414577602215846	1.4906961466770932
4	1.2497521562227867	1.0174253289360544
5	0.8444023492790621	0.8592867130613663
6	0.5720588063672378	0.698571052277343
7	0.47764424304709624	0.6804892155492742
8	0.3969092886291526	0.646249036707888
9	0.3317545418782785	0.6076843416398359
>10	5.9776544569644114	31.903674611553534
>50	2.4086805441634627	33.95538543468069
>100	0.32304274926700055	8.605760301939707
>500	0.002881780462243115	0.4030905485195281
>1k	4.5501796772259704E-4	0.1344355444020941
>5k	1.5167265590753236E-4	0.25152257538192024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8148	0.24445813879389852	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0002226165181457E-5	0.0	0.0	0.02802207923827948	0.0
2	3.0002226165181457E-5	0.0	0.0	0.10932811214592122	0.0
3	3.0002226165181457E-5	0.0	0.0	0.1671424019662259	0.0
4	3.0002226165181457E-5	0.0	0.0	0.295791947762524	0.0
5	3.0002226165181457E-5	0.0	0.0	0.5540211083662407	0.0
6	3.0002226165181457E-5	0.0	0.0	0.9654416357693741	0.0
7	3.0002226165181457E-5	0.0	0.0	1.1723969918567958	0.0
8	3.0002226165181457E-5	0.0	0.0	1.646642180849819	0.0
9	3.0002226165181457E-5	0.0	0.0	1.8123444759601162	0.0
10	3.0002226165181457E-5	0.0	0.0	2.112726764325913	0.0
11	3.0002226165181457E-5	0.0	0.0	2.4431712833092214	0.0
12	3.0002226165181457E-5	0.0	0.0	2.6817489857747443	0.0
13	3.0002226165181457E-5	0.0	0.0	2.80748831563302	0.0
14	3.0002226165181457E-5	0.0	0.0	2.874273271076714	0.0
15	3.0002226165181457E-5	0.0	0.0	2.9527290924986636	0.0
16	3.0002226165181457E-5	0.0	0.0	3.1163912362297284	0.0
17	3.0002226165181457E-5	0.0	0.0	3.304205172023764	0.0
18	3.0002226165181457E-5	0.0	0.0	3.5015298135121626	0.0
19	3.0002226165181457E-5	0.0	0.0	3.6233688539689646	0.0
20	3.0002226165181457E-5	0.0	0.0	3.7632392323510406	0.0
21	3.0002226165181457E-5	0.0	0.0	3.950693141431094	0.0
22	3.0002226165181457E-5	0.0	0.0	4.173819697421549	0.0
23	6.0004452330362914E-5	0.0	0.0	4.406276945749375	0.0
24	6.0004452330362914E-5	0.0	0.0	4.602401498191166	0.0
25	6.0004452330362914E-5	0.0	0.0	4.774974303093289	0.0
26	6.0004452330362914E-5	0.0	0.0	4.936896317706774	0.0
27	6.0004452330362914E-5	0.0	0.0	5.101248512639638	0.0
28	6.0004452330362914E-5	0.0	0.0	5.280481811750432	0.0
29	6.0004452330362914E-5	0.0	0.0	5.476096326347415	0.0
30	6.0004452330362914E-5	0.0	0.0	5.70252312721604	0.0
31	6.0004452330362914E-5	0.0	0.0	5.904288098176885	0.0
32	6.0004452330362914E-5	0.0	0.0	6.097802456942305	0.0
33	6.0004452330362914E-5	0.0	0.0	6.294167027193418	0.0
34	6.0004452330362914E-5	0.0	0.0	6.5025924923629335	0.0
35	6.0004452330362914E-5	0.0	0.0	6.723468881390999	0.0
36	6.0004452330362914E-5	0.0	0.0	6.930364233026091	0.0
37	6.0004452330362914E-5	0.0	0.0	7.14112987183649	0.0
38	6.0004452330362914E-5	0.0	0.0	7.373737131295142	0.0
39	6.0004452330362914E-5	0.0	0.0	7.680209871572471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	185	0.0	43.783783	2
CGTTTTT	6610	0.0	41.051437	1
CTACGCG	45	1.9301297E-8	40.0	1
AGGGCGA	2920	0.0	38.989723	6
GTAGGGT	3140	0.0	38.980892	4
CGTAAGG	560	0.0	38.571426	2
GGGCGAT	5480	0.0	38.55383	7
TACGGGT	795	0.0	38.207546	4
CGTTAGG	555	0.0	38.10811	2
TAGGGTC	2970	0.0	37.803032	5
CGAGGGT	1465	0.0	37.78157	4
GGCGATA	960	0.0	37.73437	8
TAGGGTA	2450	0.0	37.653065	5
GACCGAT	1805	0.0	37.645428	9
TATGGGC	1705	0.0	36.81818	4
TAAGGGA	3920	0.0	36.734695	4
ATAGGGT	3110	0.0	36.60772	4
TAGGGCG	1305	0.0	36.37931	5
AGGGTAC	2025	0.0	36.333332	6
GCGATGT	795	0.0	36.226414	9
>>END_MODULE