FastQCFastQC Report
Thu 26 May 2016
SRR1548005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548005_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2780983
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179130.6441247573250178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127150.042.0625271
TTTACGG3000.039.02
AGGGCGA25950.038.9306346
GGGCGAT50150.038.359927
AAGGGAT55900.038.0769235
TAAGGGA41550.037.9061364
ACGGGAT15050.037.674425
TACGGGA13650.037.4175834
TTAGGGA46100.037.3861164
AGGGATT56450.037.347216
GACCGAT14950.037.3244139
TAGGGAC30550.037.0458265
ATAGGGA43050.036.8989564
CACGACC4650.036.77419327
TAGGGAT52300.036.739965
TGTTACG1350.036.6666641
GGCGATA12950.036.6602338
AGGGACC35650.036.6058926
GGCACCG12900.036.4534878
AGGGATC39000.036.3461536
Produced by FastQC (version 0.11.5)