##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548005_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2780983
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92340262418001	31.0	31.0	33.0	30.0	33.0
2	31.25505657531887	31.0	31.0	33.0	30.0	34.0
3	31.32874850367658	31.0	31.0	34.0	30.0	34.0
4	35.28157633469892	37.0	35.0	37.0	33.0	37.0
5	35.1657838253596	37.0	35.0	37.0	32.0	37.0
6	35.21760039525592	37.0	35.0	37.0	32.0	37.0
7	35.571438228856486	36.0	35.0	37.0	35.0	37.0
8	35.66321692725198	37.0	35.0	37.0	35.0	37.0
9	37.50787976769365	39.0	37.0	39.0	35.0	39.0
10	36.67920659709175	39.0	37.0	39.0	32.0	39.0
11	36.29004168669855	38.0	35.0	39.0	31.0	39.0
12	35.905894066954026	37.0	35.0	39.0	31.0	39.0
13	35.75330917161306	37.0	35.0	39.0	30.0	39.0
14	36.785136766388	39.0	35.0	40.0	31.0	41.0
15	36.939050328606825	39.0	35.0	41.0	31.0	41.0
16	37.02511665839022	39.0	35.0	41.0	31.0	41.0
17	36.95464049942053	39.0	35.0	41.0	31.0	41.0
18	36.82791696317453	38.0	35.0	40.0	31.0	41.0
19	36.747544303578984	38.0	35.0	40.0	31.0	41.0
20	36.60313529424668	38.0	35.0	40.0	31.0	41.0
21	36.48449163479245	38.0	35.0	40.0	30.0	41.0
22	36.37831083469406	38.0	35.0	40.0	30.0	41.0
23	36.283509823684646	38.0	35.0	40.0	30.0	41.0
24	36.17117040988744	38.0	34.0	40.0	30.0	41.0
25	36.01608711739698	38.0	34.0	40.0	30.0	41.0
26	35.80853460808642	38.0	34.0	40.0	29.0	41.0
27	35.88171808313823	38.0	34.0	40.0	30.0	41.0
28	35.89587674574062	38.0	34.0	40.0	30.0	41.0
29	35.879363160436434	38.0	34.0	40.0	30.0	41.0
30	35.78421011563177	38.0	34.0	40.0	29.0	41.0
31	35.67584519574553	38.0	34.0	40.0	29.0	41.0
32	35.50551765329022	38.0	34.0	40.0	28.0	41.0
33	35.30453008882111	38.0	34.0	40.0	27.0	41.0
34	35.05670944410664	38.0	34.0	40.0	25.0	41.0
35	34.85268518362032	38.0	33.0	40.0	24.0	41.0
36	34.76046779142483	38.0	33.0	40.0	24.0	41.0
37	34.706936719857694	38.0	33.0	40.0	24.0	41.0
38	34.618590620654636	38.0	33.0	40.0	24.0	41.0
39	34.57316351807976	38.0	33.0	40.0	23.0	41.0
40	34.4199504276006	37.0	33.0	40.0	23.0	41.0
41	34.32599875655479	37.0	33.0	40.0	23.0	41.0
42	34.28949331944855	37.0	33.0	40.0	23.0	41.0
43	34.251292079095776	37.0	33.0	40.0	23.0	41.0
44	34.11091509728754	37.0	33.0	40.0	23.0	41.0
45	33.98231200981811	37.0	33.0	40.0	23.0	41.0
46	33.93177340530309	37.0	33.0	40.0	23.0	41.0
47	33.87487302151793	37.0	33.0	40.0	22.0	41.0
48	33.77226002460281	36.0	33.0	40.0	22.0	41.0
49	33.64378494942256	36.0	32.0	40.0	22.0	41.0
50	33.53406115751157	36.0	32.0	40.0	21.0	41.0
51	33.1966272357652	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	8.0
13	12.0
14	29.0
15	91.0
16	261.0
17	697.0
18	1597.0
19	3087.0
20	5276.0
21	8533.0
22	12856.0
23	18895.0
24	28056.0
25	40511.0
26	52853.0
27	59777.0
28	64746.0
29	72776.0
30	85936.0
31	102213.0
32	123854.0
33	157291.0
34	224568.0
35	259755.0
36	242628.0
37	299859.0
38	411504.0
39	503267.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.80004444471613	25.14912892311819	29.179682148362645	14.871144483803029
2	32.10041197662841	26.09512535675335	28.88273678767544	12.921725878942805
3	27.961695558728692	26.085668269097656	32.5063835341676	13.446252638006056
4	24.53517335417009	28.559793425562113	32.47261849497102	14.432414725296775
5	22.5456610126707	32.390201594184504	31.154595335534236	13.909542057610564
6	21.426632237593683	41.31862726237449	27.438535222976913	9.816205277054912
7	89.52107941688244	2.7092578415617785	6.757754362396318	1.0119083791594554
8	90.28268062048564	2.1476938190560677	6.508993402692501	1.0606321577657971
9	85.27610560726188	5.185504550009835	7.744311993277197	1.7940778494510752
10	38.50814622023939	38.38441299353502	13.43305586549792	9.67438492072767
11	29.912480586900386	25.162253778609937	28.34569646775978	16.579569166729893
12	29.596189548803427	23.131029567602535	29.307802313067	17.96497857052704
13	23.931358084533418	27.287940990649712	30.326794518341178	18.453906406475696
14	19.82367385920734	29.65203311203269	30.800404029798095	19.72388899896188
15	19.34952497012747	26.90789551752024	35.82956098616928	17.91301852618301
16	23.26400413091342	25.25038808219971	33.96662259352179	17.51898519336508
17	22.887554508603614	25.24118270410139	29.744050934507687	22.12721185278731
18	23.238905092192223	25.864883028770763	31.821086284957513	19.075125594079502
19	25.455711164002082	27.354176562747778	28.152347569186865	19.037764704063274
20	27.020481606683678	27.065213990880206	28.4678475201035	17.446456882332615
21	25.26692899597013	26.326913900588387	30.58947142071706	17.81668568272442
22	24.84344564493922	24.066526116844294	30.16192475825994	20.928103479956548
23	22.049289765525355	27.467337988042356	30.109497253309353	20.373874993122936
24	21.57492512539631	26.361613860998073	33.332566218491806	18.730894795113816
25	21.522425703429327	28.292298083087886	30.390872579947448	19.79440363353534
26	20.881896796923964	30.41794933661946	28.24486881077662	20.455285055679955
27	20.057907581599743	29.615786935770554	30.259480190997213	20.066825291632494
28	19.047077957686184	29.063572125395947	32.558882956134575	19.33046696078329
29	20.39839869571299	26.843565746356596	32.161038021447816	20.596997536482604
30	21.17201004105383	27.75306429417224	31.439854181057562	19.635071483716366
31	23.89820433997619	27.491106561960287	29.016969898773205	19.593719199290323
32	24.574583879153522	26.950434432716776	29.030957758461668	19.444023929668035
33	23.76709961909152	27.797796678368762	28.280359858366626	20.154743844173083
34	21.011095716874213	28.148464050301637	30.05534373996533	20.78509649285882
35	21.06208488149694	28.361230543300696	30.161673048702564	20.4150115264998
36	23.55469271117443	28.959759912232474	27.852489569335738	19.633057807257362
37	21.774207177821655	29.988137288145957	28.548430536971996	19.689224997060393
38	21.551839763134115	30.235603741554694	27.703153884795412	20.509402610515778
39	21.338282182954732	29.341099891657013	28.37230576382524	20.948312161563017
40	23.23192914160209	27.762305630778755	27.875574931597928	21.130190296021226
41	19.922487839731488	27.665685119254597	29.23279286496897	23.179034176044947
42	21.31613174190565	28.08985168194124	27.900278426728963	22.693738149424142
43	21.164602588365337	27.89948733954864	28.606647361742233	22.32926271034379
44	21.06895295656248	28.824627838429794	28.515384667939358	21.591034537068367
45	20.512279291171502	29.82416649076963	27.443245787550662	22.220308430508204
46	21.52461917242932	29.99295572824429	27.508510479927423	20.97391461939897
47	20.73479053989183	28.77719856611853	28.939119728527647	21.548891165462
48	21.47010607400333	27.881580002466755	29.77123556670429	20.877078356825628
49	21.762268953100396	27.526777402091273	29.276446493919593	21.43450715088873
50	20.624361961220185	29.47317549226299	28.76882023370873	21.133642312808096
51	19.91547593063316	29.85706852576949	28.263027857415885	21.96442768618147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1850.0
1	2459.5
2	3069.0
3	18117.5
4	33166.0
5	25767.0
6	18368.0
7	17206.5
8	16045.0
9	16571.5
10	17098.0
11	17226.0
12	17354.0
13	17250.5
14	17147.0
15	16592.5
16	16038.0
17	15463.5
18	14889.0
19	14642.5
20	14396.0
21	15703.0
22	17010.0
23	18163.5
24	19317.0
25	21955.0
26	29708.5
27	34824.0
28	41941.5
29	49059.0
30	55331.0
31	61603.0
32	69621.0
33	77639.0
34	86518.5
35	95398.0
36	105879.5
37	116361.0
38	123678.0
39	130995.0
40	145983.5
41	160972.0
42	175977.0
43	190982.0
44	196764.5
45	202547.0
46	202601.0
47	202655.0
48	199211.0
49	195767.0
50	190304.0
51	184841.0
52	173719.5
53	162598.0
54	153633.0
55	144668.0
56	136301.0
57	127934.0
58	119407.0
59	110880.0
60	100388.0
61	89896.0
62	80455.0
63	71014.0
64	59007.5
65	47001.0
66	39154.0
67	31307.0
68	26637.5
69	21968.0
70	18773.5
71	15579.0
72	12730.5
73	9882.0
74	8281.0
75	5280.5
76	3881.0
77	2867.0
78	1853.0
79	1421.0
80	989.0
81	729.0
82	469.0
83	357.5
84	246.0
85	162.5
86	79.0
87	59.0
88	39.0
89	30.5
90	22.0
91	17.0
92	12.0
93	7.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2780983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.453111278719813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00431929582547	16.840803275338008
2	6.559966645275432	2.945833221421191
3	2.6891349748655826	1.8113834050246302
4	1.5517232995982602	1.3936406367864809
5	1.101557642925694	1.2366698168267456
6	0.8609185551516132	1.159818007244031
7	0.7391650217904082	1.1617588141318174
8	0.6088616550853115	1.0936670795980816
9	0.5295888739946208	1.070182612779583
>10	8.998098259642271	49.989425898643425
>50	1.2969615986784537	18.327459579145234
>100	0.05838506225950439	2.0170573906652565
>500	3.297787268401443E-4	0.040206752389452934
>1k	8.244468171003607E-4	0.24890423233360223
>5k	0.0	0.0
>10k+	1.6488936342007213E-4	0.6631892776724404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17913	0.6441247573250178	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01636112122943578	0.0
2	0.0	0.0	0.0	0.05397372080303979	0.0
3	0.0	0.0	0.0	0.09600921688482095	0.0
4	0.0	0.0	0.0	0.1459196262616492	0.0
5	0.0	0.0	0.0	0.2682864296545502	0.0
6	0.0	0.0	0.0	0.5005064755879486	0.0
7	0.0	0.0	0.0	0.6145668635874437	0.0
8	0.0	0.0	0.0	0.8362510666192494	0.0
9	0.0	0.0	0.0	0.9687222108153843	0.0
10	0.0	0.0	0.0	1.160273183978471	0.0
11	0.0	0.0	0.0	1.3941113627807147	0.0
12	0.0	0.0	0.0	1.5653457788127436	0.0
13	0.0	0.0	0.0	1.674120266107344	0.0
14	0.0	0.0	0.0	1.7515389342545424	0.0
15	0.0	0.0	0.0	1.8182419669591652	0.0
16	0.0	0.0	0.0	1.9219822631062469	0.0
17	0.0	0.0	0.0	2.0449603611384894	0.0
18	0.0	0.0	0.0	2.1785821775969145	0.0
19	0.0	0.0	0.0	2.2763174028751703	0.0
20	0.0	0.0	0.0	2.3741964621862124	0.0
21	0.0	0.0	0.0	2.50246046092335	0.0
22	0.0	0.0	0.0	2.6433458960374803	0.0
23	0.0	0.0	0.0	2.804547888282668	0.0
24	0.0	0.0	0.0	2.9324163434296433	0.0
25	0.0	0.0	0.0	3.0499287482160087	0.0
26	0.0	0.0	0.0	3.163377841576162	0.0
27	0.0	0.0	0.0	3.29038329252642	0.0
28	0.0	0.0	0.0	3.4175685360176598	0.0
29	0.0	0.0	0.0	3.5606833986399775	0.0
30	0.0	0.0	0.0	3.728753465950709	0.0
31	0.0	0.0	0.0	3.876542934638579	0.0
32	0.0	0.0	0.0	4.022390643883836	0.0
33	0.0	0.0	0.0	4.168849647768433	0.0
34	0.0	0.0	0.0	4.318760668439901	0.0
35	0.0	0.0	0.0	4.492512180045689	0.0
36	0.0	0.0	0.0	4.65378608930727	0.0
37	0.0	0.0	0.0	4.821316778995053	0.0
38	0.0	0.0	0.0	5.011681121387653	0.0
39	0.0	0.0	0.0	5.267418031681603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12715	0.0	42.062527	1
TTTACGG	300	0.0	39.0	2
AGGGCGA	2595	0.0	38.930634	6
GGGCGAT	5015	0.0	38.35992	7
AAGGGAT	5590	0.0	38.076923	5
TAAGGGA	4155	0.0	37.906136	4
ACGGGAT	1505	0.0	37.67442	5
TACGGGA	1365	0.0	37.417583	4
TTAGGGA	4610	0.0	37.386116	4
AGGGATT	5645	0.0	37.34721	6
GACCGAT	1495	0.0	37.324413	9
TAGGGAC	3055	0.0	37.045826	5
ATAGGGA	4305	0.0	36.898956	4
CACGACC	465	0.0	36.774193	27
TAGGGAT	5230	0.0	36.73996	5
TGTTACG	135	0.0	36.666664	1
GGCGATA	1295	0.0	36.660233	8
AGGGACC	3565	0.0	36.605892	6
GGCACCG	1290	0.0	36.453487	8
AGGGATC	3900	0.0	36.346153	6
>>END_MODULE