FastQCFastQC Report
Thu 26 May 2016
SRR1548004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548004_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2376973
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.28649883696617506No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24960.10500750324046591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA8850.039.661024
AGGGCGA20850.039.388496
GCGATAC1400.038.571439
CGTTTTT42100.038.10571
GGGCGAT39950.038.072597
GGCGATA10050.038.05978
AGTACGG3150.037.8571432
CGTTAGG3400.037.720592
TAAGGGA30300.037.6485144
GTTGATC8200.037.59146516
GTACGGG9300.037.53
TCACGAC2800.036.96428725
GCGATAT2800.036.9642879
CGGGATA5700.036.7105266
ATAGGGC10900.036.5366974
ATAGGGA39550.036.4096074
AACGGGA9400.036.382984
ACGGGAT11100.036.2837835
GTAGGGT9100.036.09894
TACGCAA250.002107560236.013
Produced by FastQC (version 0.11.5)