##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548004_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2376973
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89865345546626	31.0	31.0	31.0	30.0	33.0
2	31.251951536681318	31.0	31.0	33.0	30.0	34.0
3	31.46462117996292	31.0	31.0	34.0	30.0	34.0
4	35.34538171026764	37.0	35.0	37.0	33.0	37.0
5	35.1601684158802	37.0	35.0	37.0	32.0	37.0
6	35.18335336581442	37.0	35.0	37.0	32.0	37.0
7	35.49707001299552	35.0	35.0	37.0	35.0	37.0
8	35.56806787456147	37.0	35.0	37.0	35.0	37.0
9	37.415439300320195	39.0	37.0	39.0	35.0	39.0
10	36.59153006786362	39.0	35.0	39.0	32.0	39.0
11	36.239034267532695	38.0	35.0	39.0	31.0	39.0
12	35.63473459732189	37.0	35.0	39.0	30.0	39.0
13	35.348406145126596	37.0	35.0	39.0	30.0	39.0
14	36.2876620811427	38.0	35.0	40.0	30.0	41.0
15	36.54197207961555	38.0	35.0	40.0	30.0	41.0
16	36.688398227493536	38.0	35.0	40.0	31.0	41.0
17	36.62624438729426	38.0	35.0	40.0	31.0	41.0
18	36.550662123633714	38.0	35.0	40.0	31.0	41.0
19	36.48518935637889	38.0	35.0	40.0	30.0	41.0
20	36.356655712959295	38.0	35.0	40.0	30.0	41.0
21	36.219087890354665	38.0	34.0	40.0	30.0	41.0
22	36.11540938832709	38.0	34.0	40.0	30.0	41.0
23	36.05725306934492	38.0	34.0	40.0	30.0	41.0
24	35.93785962230114	38.0	34.0	40.0	30.0	41.0
25	35.74954532508362	38.0	34.0	40.0	29.0	41.0
26	35.509620849710956	37.0	34.0	40.0	28.0	41.0
27	35.608312757444025	38.0	34.0	40.0	29.0	41.0
28	35.63701817395486	38.0	34.0	40.0	29.0	41.0
29	35.61726784443913	38.0	34.0	40.0	29.0	41.0
30	35.54028127370399	38.0	34.0	40.0	29.0	41.0
31	35.4520084998862	38.0	34.0	40.0	28.0	41.0
32	35.330324324256104	37.0	34.0	40.0	27.0	41.0
33	35.20490135983875	37.0	34.0	40.0	27.0	41.0
34	35.068095851320145	37.0	34.0	40.0	26.0	41.0
35	34.94139983920726	37.0	33.0	40.0	25.0	41.0
36	34.852992019682176	37.0	33.0	40.0	25.0	41.0
37	34.798427243388964	37.0	33.0	40.0	25.0	41.0
38	34.72363421881528	37.0	33.0	40.0	25.0	41.0
39	34.66879430266982	37.0	33.0	40.0	24.0	41.0
40	34.44835469313282	37.0	33.0	40.0	24.0	41.0
41	34.3504608592525	37.0	33.0	40.0	23.0	41.0
42	34.308755715778005	37.0	33.0	40.0	23.0	41.0
43	34.26620411758989	36.0	33.0	40.0	23.0	41.0
44	34.116900780951234	36.0	33.0	40.0	23.0	41.0
45	33.99027628837181	36.0	33.0	40.0	23.0	41.0
46	33.95380216771499	36.0	33.0	40.0	23.0	41.0
47	33.8896722848766	36.0	33.0	40.0	23.0	41.0
48	33.784232298810295	35.0	32.0	40.0	23.0	41.0
49	33.643080506173185	35.0	32.0	40.0	23.0	41.0
50	33.520269266836436	35.0	32.0	39.0	23.0	41.0
51	33.18652462606853	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	6.0
13	8.0
14	47.0
15	129.0
16	325.0
17	776.0
18	1768.0
19	3076.0
20	5205.0
21	8041.0
22	11640.0
23	16489.0
24	23343.0
25	30916.0
26	39290.0
27	47004.0
28	54738.0
29	65432.0
30	77821.0
31	93388.0
32	114656.0
33	144842.0
34	212302.0
35	240985.0
36	205251.0
37	251784.0
38	336165.0
39	391494.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.61084581103782	25.517580553081586	29.595498139861075	12.276075496019518
2	29.278666606646354	27.275867247966218	29.935636626920036	13.509829518467395
3	29.097301483862037	26.57198882780747	30.476029807658733	13.85467988067176
4	25.93836783169182	29.476060518987808	30.159029993188817	14.426541656131558
5	23.390084784303397	33.471141657898514	28.834446163250487	14.304327394547602
6	22.0453492740557	42.52652428109196	25.104660423151632	10.32346602170071
7	91.6434894296233	3.1272126355663272	4.141191338732077	1.0881065960782894
8	92.16646550044952	2.7242210996927607	3.7544810142984373	1.3548323855592805
9	87.48513340286154	5.543773530452387	5.049825976146974	1.9212670905391016
10	42.27485966395075	35.673690866492805	11.062515224194806	10.988934245361643
11	37.45284443702137	24.370954150509913	22.04391888338656	16.132282529082158
12	35.09017561411088	22.085189861222656	24.95442733257803	17.870207192088426
13	26.286373467431055	28.385808336905804	26.54834531145284	18.779472884210296
14	20.60524036242734	31.25992596466178	28.471673847368063	19.663159825542824
15	19.677674083803222	25.75010317744459	36.04950497965269	18.522717759099493
16	24.610502517277226	23.604138540908963	33.37395923302452	18.41139970878929
17	24.471754622370554	23.734177880859395	26.314602647989695	25.47946484878036
18	25.378790587861115	25.237602614754145	29.226457347222706	20.157149450162034
19	28.448156541954834	26.1011799460911	25.444883050838186	20.005780461115883
20	30.423946759176484	26.46925312151211	24.799903069997008	18.3068970493144
21	27.63169796207193	25.832560992489185	27.622190071153526	18.91355097428536
22	26.814818679051044	22.993067232989183	27.176455096460916	23.015658991498853
23	24.04158566378331	26.81549180407182	26.719361137042785	22.42356139510209
24	23.451717794017853	25.139200150780006	31.11061842099174	20.2984636342104
25	23.14393137826976	26.153936119594125	28.913496282877425	21.788636219258695
26	22.096254353751597	30.050025810137516	25.965124551267515	21.88859528484337
27	21.36082319824415	29.876359554778283	27.860518398820684	20.902298848156878
28	20.082348432228724	28.75619537958572	30.81431720091057	20.34713898727499
29	21.553715587009194	26.4961360520292	30.474978049813778	21.475170311147835
30	22.58132507184558	27.125676227706414	29.30710613877398	20.985892561674028
31	26.761347310213452	26.882341532697257	25.781740053420883	20.574571103668404
32	27.694214448376147	26.067229202855902	26.434376831373346	19.8041795173946
33	26.72318953559843	26.819614694824047	25.53571285832864	20.921482911248887
34	22.409888543117653	27.526816669772856	28.491110332342856	21.572184454766628
35	22.830507540472695	26.856468289711326	28.64327865735118	21.669745512464804
36	26.52886675616425	26.687934612635484	26.15612377591163	20.627074855288637
37	23.40409419879822	29.49966196502863	26.675481799751193	20.42076203642195
38	23.543431078098067	29.228729144167815	25.100537532399404	22.127302245334718
39	22.95196453640828	28.008521762762978	26.41207956506027	22.627434135768475
40	25.02274952218641	25.88868279109607	26.35267628197712	22.7358914047404
41	21.351062885442957	25.097634680747323	27.976337972707306	25.574964461102418
42	23.190755637527225	25.820781304625672	26.324489171732285	24.663973886114817
43	22.88801765943492	25.65632844798826	27.56026256924248	23.89539132333434
44	21.84139239276172	27.41995807272527	27.790387185718977	22.94826234879403
45	21.831590009646725	29.343665241464667	25.52094617818545	23.30379857070316
46	22.81288007899122	28.230526808676416	26.384018665756827	22.572574446575537
47	22.197012755298438	27.603342570571897	27.46021936303021	22.739425311099453
48	23.33047114965126	25.939167167653988	29.254981019977933	21.475380662716827
49	23.064460555504837	25.21660952816881	28.896331594847734	22.822598321478623
50	21.706557037038284	28.844416827620677	27.452015651839545	21.997010483501494
51	21.308235305996323	29.489775441286035	26.207449558745516	22.994539693972126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1743.0
1	2237.0
2	2731.0
3	7909.5
4	13088.0
5	10164.0
6	7240.0
7	6986.0
8	6732.0
9	7184.5
10	7637.0
11	7768.5
12	7900.0
13	7823.5
14	7747.0
15	7889.5
16	8032.0
17	8013.5
18	7995.0
19	8444.5
20	8894.0
21	9282.5
22	9671.0
23	10146.5
24	10622.0
25	13435.5
26	20620.0
27	24991.0
28	28595.0
29	32199.0
30	35776.5
31	39354.0
32	46801.5
33	54249.0
34	61147.0
35	68045.0
36	73366.5
37	78688.0
38	87985.0
39	97282.0
40	116064.5
41	134847.0
42	145382.5
43	155918.0
44	161782.0
45	167646.0
46	171061.5
47	174477.0
48	170713.0
49	166949.0
50	165012.0
51	163075.0
52	155965.5
53	148856.0
54	142102.0
55	135348.0
56	131872.0
57	128396.0
58	123778.0
59	119160.0
60	112443.0
61	105726.0
62	93922.0
63	82118.0
64	71304.0
65	60490.0
66	51604.0
67	42718.0
68	36090.0
69	29462.0
70	24287.5
71	19113.0
72	16048.0
73	12983.0
74	10711.5
75	6420.0
76	4400.0
77	3619.0
78	2838.0
79	2118.0
80	1398.0
81	1140.5
82	883.0
83	607.0
84	331.0
85	234.0
86	137.0
87	123.0
88	109.0
89	74.5
90	40.0
91	28.0
92	16.0
93	11.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2376973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.833671735427966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13751640505826	14.716163341944846
2	6.276387372828742	2.364148389284833
3	2.130960124472144	1.2040141039678522
4	1.1949824396156137	0.9002362798928536
5	0.7986349225201262	0.7520613983596502
6	0.6141244584483343	0.6939731073068041
7	0.4892379994620609	0.6449903517656157
8	0.41221792348454567	0.6210861643494157
9	0.35232119398216344	0.5971951541604694
>10	6.536976965547474	32.482880024259416
>50	2.6554493868050435	34.76110545954811
>100	0.39867478917419596	9.52684678151416
>500	0.0016011027462797189	0.22314436218212083
>1k	6.861868912627368E-4	0.21879347790019146
>5k	2.2872896375424555E-4	0.29336160356375346
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6810	0.28649883696617506	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2496	0.10500750324046591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.207031379826359E-5	0.0	0.0	0.009213398721819727	0.0
2	4.207031379826359E-5	0.0	0.0	0.034497657314576144	0.0
3	4.207031379826359E-5	0.0	0.0	0.05826738461059507	0.0
4	4.207031379826359E-5	0.0	0.0	0.11014008152385407	0.0
5	4.207031379826359E-5	0.0	0.0	0.19651043575168922	0.0
6	4.207031379826359E-5	0.0	0.0	0.34678559663908676	0.0
7	4.207031379826359E-5	0.0	0.0	0.41392981746111546	0.0
8	4.207031379826359E-5	0.0	0.0	0.5712727910666213	0.0
9	4.207031379826359E-5	0.0	0.0	0.6411095119717388	0.0
10	4.207031379826359E-5	0.0	0.0	0.758443617155096	0.0
11	8.414062759652717E-5	0.0	0.0	0.9200356924542265	0.0
12	8.414062759652717E-5	0.0	0.0	1.0466252666732017	0.0
13	8.414062759652717E-5	0.0	0.0	1.115157807850573	0.0
14	8.414062759652717E-5	0.0	0.0	1.156807418510854	0.0
15	8.414062759652717E-5	0.0	0.0	1.2022433574129785	0.0
16	8.414062759652717E-5	0.0	0.0	1.2855005084197422	0.0
17	8.414062759652717E-5	0.0	0.0	1.3778869175207291	0.0
18	8.414062759652717E-5	0.0	0.0	1.4850400067649066	0.0
19	8.414062759652717E-5	0.0	0.0	1.556307118339165	0.0
20	8.414062759652717E-5	0.0	0.0	1.634515831690137	0.0
21	8.414062759652717E-5	0.0	0.0	1.731866537819319	0.0
22	8.414062759652717E-5	0.0	0.0	1.8327932206213533	0.0
23	8.414062759652717E-5	0.0	0.0	1.9486548648217712	0.0
24	8.414062759652717E-5	0.0	0.0	2.0444068990266193	0.0
25	8.414062759652717E-5	0.0	0.0	2.1261915890504435	0.0
26	8.414062759652717E-5	0.0	0.0	2.2139502636336212	0.0
27	8.414062759652717E-5	0.0	0.0	2.310669915055829	0.0
28	8.414062759652717E-5	0.0	0.0	2.403603238236194	0.0
29	8.414062759652717E-5	0.0	0.0	2.510419764969985	0.0
30	8.414062759652717E-5	0.0	0.0	2.6540057459634587	0.0
31	8.414062759652717E-5	0.0	0.0	2.7789966482581	0.0
32	8.414062759652717E-5	0.0	0.0	2.8918292298650425	0.0
33	8.414062759652717E-5	0.0	0.0	3.0051666552375647	0.0
34	8.414062759652717E-5	0.0	0.0	3.1352059951879974	0.0
35	8.414062759652717E-5	0.0	0.0	3.283587991954473	0.0
36	8.414062759652717E-5	0.0	0.0	3.4070222926385787	0.0
37	8.414062759652717E-5	0.0	0.0	3.5457281172314534	0.0
38	1.2621094139479076E-4	0.0	0.0	3.7219606617323797	0.0
39	1.2621094139479076E-4	0.0	0.0	4.038792194947103	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	885	0.0	39.66102	4
AGGGCGA	2085	0.0	39.38849	6
GCGATAC	140	0.0	38.57143	9
CGTTTTT	4210	0.0	38.1057	1
GGGCGAT	3995	0.0	38.07259	7
GGCGATA	1005	0.0	38.0597	8
AGTACGG	315	0.0	37.857143	2
CGTTAGG	340	0.0	37.72059	2
TAAGGGA	3030	0.0	37.648514	4
GTTGATC	820	0.0	37.591465	16
GTACGGG	930	0.0	37.5	3
TCACGAC	280	0.0	36.964287	25
GCGATAT	280	0.0	36.964287	9
CGGGATA	570	0.0	36.710526	6
ATAGGGC	1090	0.0	36.536697	4
ATAGGGA	3955	0.0	36.409607	4
AACGGGA	940	0.0	36.38298	4
ACGGGAT	1110	0.0	36.283783	5
GTAGGGT	910	0.0	36.0989	4
TACGCAA	25	0.0021075602	36.0	13
>>END_MODULE