FastQCFastQC Report
Thu 26 May 2016
SRR1548003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548003_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2817437
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148060.5255130815702357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36520.12962135444377282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA13500.041.1666684
CGTTTTT86900.041.1421171
TTCGTAG2000.040.51
CGGTCTA1300.039.8076931
CGGCTAC5150.039.3203925
TAAGGGA50900.039.165034
ATTAGCG2200.038.8636361
ACGGGAT15550.038.7781375
GGGCGAT51800.038.441127
CTCGTCC4450.038.42696437
TCGTCCC4400.038.35227238
TGGGCGA15050.038.2724236
ACGGGAC10500.038.1428575
AGGGCGA24350.037.7926066
TAGGGCG8350.037.7245525
ACGCGAG301.1403425E-437.5000041
GCGCGTT301.1403425E-437.50000431
AAGGGAT56950.037.295875
AGGGATC38950.037.2593086
CGACGGT1450.037.2413828
Produced by FastQC (version 0.11.5)