##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548003_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2817437
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.889635154219953	31.0	31.0	31.0	30.0	33.0
2	31.23180216629511	31.0	31.0	33.0	30.0	34.0
3	31.371607954321604	31.0	31.0	34.0	30.0	34.0
4	35.25279961894445	37.0	35.0	37.0	33.0	37.0
5	35.092947952341085	35.0	35.0	37.0	32.0	37.0
6	35.129833604087686	37.0	35.0	37.0	32.0	37.0
7	35.56774508178887	36.0	35.0	37.0	35.0	37.0
8	35.67012749530868	37.0	35.0	37.0	35.0	37.0
9	37.490804585870066	39.0	37.0	39.0	35.0	39.0
10	36.614971337424755	39.0	35.0	39.0	32.0	39.0
11	36.145935472558925	38.0	35.0	39.0	31.0	39.0
12	35.72694864162003	37.0	35.0	39.0	30.0	39.0
13	35.5147781476569	37.0	35.0	39.0	30.0	39.0
14	36.549888781896456	38.0	35.0	40.0	30.0	41.0
15	36.74357581021332	38.0	35.0	40.0	31.0	41.0
16	36.85867545574222	38.0	35.0	40.0	31.0	41.0
17	36.792608672350084	38.0	35.0	40.0	31.0	41.0
18	36.6903458710878	38.0	35.0	40.0	31.0	41.0
19	36.63947800784898	38.0	35.0	40.0	31.0	41.0
20	36.51770527610733	38.0	35.0	40.0	30.0	41.0
21	36.40013494534217	38.0	35.0	40.0	30.0	41.0
22	36.27215763830744	38.0	34.0	40.0	30.0	41.0
23	36.19209267145991	38.0	34.0	40.0	30.0	41.0
24	36.09574127123339	38.0	34.0	40.0	30.0	41.0
25	35.94137437678287	38.0	34.0	40.0	30.0	41.0
26	35.704146712064905	38.0	34.0	40.0	29.0	41.0
27	35.778637108833315	38.0	34.0	40.0	29.0	41.0
28	35.79779565612292	38.0	34.0	40.0	29.0	41.0
29	35.80848906293202	38.0	34.0	40.0	29.0	41.0
30	35.74563441879978	38.0	34.0	40.0	29.0	41.0
31	35.66387322946352	38.0	34.0	40.0	29.0	41.0
32	35.525806610760064	38.0	34.0	40.0	29.0	41.0
33	35.38329730176753	38.0	34.0	40.0	27.0	41.0
34	35.23265826352107	38.0	34.0	40.0	27.0	41.0
35	35.093257098561565	38.0	34.0	40.0	26.0	41.0
36	35.00995834157072	38.0	34.0	40.0	25.0	41.0
37	34.960651826464975	38.0	34.0	40.0	25.0	41.0
38	34.88815473070028	38.0	33.0	40.0	25.0	41.0
39	34.86623516337721	37.0	34.0	40.0	25.0	41.0
40	34.70181906463215	37.0	33.0	40.0	24.0	41.0
41	34.644813708345566	37.0	33.0	40.0	24.0	41.0
42	34.611574633257106	37.0	33.0	40.0	24.0	41.0
43	34.58049283799425	37.0	33.0	40.0	24.0	41.0
44	34.42854977768802	37.0	33.0	40.0	24.0	41.0
45	34.32992361497347	37.0	33.0	40.0	23.0	41.0
46	34.282365497436146	37.0	33.0	40.0	23.0	41.0
47	34.24643709868224	37.0	33.0	40.0	23.0	41.0
48	34.15999257481179	37.0	33.0	40.0	23.0	41.0
49	34.03664003844629	36.0	33.0	40.0	23.0	41.0
50	33.90947410714064	36.0	33.0	40.0	23.0	41.0
51	33.57594508768075	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	4.0
13	8.0
14	37.0
15	102.0
16	245.0
17	698.0
18	1505.0
19	2914.0
20	4955.0
21	8000.0
22	11944.0
23	17822.0
24	25554.0
25	35948.0
26	46550.0
27	54550.0
28	62679.0
29	74153.0
30	88813.0
31	106942.0
32	130626.0
33	165153.0
34	240036.0
35	281724.0
36	242541.0
37	292956.0
38	403780.0
39	517137.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.56291941931621	25.699101701298023	30.397733826878827	13.34024505250694
2	28.97438345560167	27.32000751037201	30.441780951978696	13.26382808204762
3	27.863196231184585	26.190399288431294	32.209202903206	13.737201577178123
4	24.376445684499778	29.498086381345885	31.824065631281194	14.301402302873143
5	22.756746645976467	33.70953813696633	29.908175409068598	13.625539807988607
6	21.81170333178701	41.93818708279901	26.338512626901682	9.911596958512293
7	91.18695466837413	2.9516897804635915	4.995604160802886	0.865751390359394
8	91.99740757291113	2.2846651052002227	4.577422671740309	1.140504650148344
9	87.28734662035033	5.495491114796888	5.649247880254288	1.5679143845984844
10	41.21838394256908	39.98467401400635	10.219642888199452	8.577299155225122
11	29.54546987208587	26.530673090471947	27.627556534538307	16.29630050290388
12	30.003155350057515	24.856278951401574	27.915584270384752	17.22498142815616
13	22.85520492561147	31.116365689809566	28.579485539516945	17.448943845062022
14	18.132863308034928	34.22344492529913	29.139923980553956	18.50376778611199
15	17.061251059029892	28.91493225935487	37.51331440596542	16.51050227564982
16	21.022688351150354	26.679141361457237	35.43937273486505	16.85879755252735
17	20.5222335051325	26.471967252506445	28.6735781492186	24.332221093142454
18	21.96709278681298	28.161304050454365	31.19807115474099	18.67353200799166
19	25.32990089929251	28.702256696423024	27.193225616047496	18.77461678823697
20	27.095690160951246	28.899563681459426	26.968659813866292	17.036086343723035
21	24.159297971880118	28.62953102411873	29.211513868810552	17.9996571351906
22	23.583029540678282	26.197036526460042	29.063684476352087	21.156249456509588
23	20.492277200874412	30.58677088431791	28.332310536136212	20.588641378671465
24	20.25748934226391	28.308920483403888	33.256963687209335	18.17662648712287
25	19.967864410100386	30.093769621113093	30.49686647829215	19.441499490494373
26	19.391631472150042	34.07543806658321	27.528707829136906	19.004222632129842
27	19.023885893455645	32.883077776007056	29.841448096266216	18.251588234271075
28	17.346936240277955	31.363611679693282	33.194779510597755	18.09467256943101
29	18.342344478332613	28.663178626531842	32.90582895021255	20.08864794492299
30	19.896629454358695	30.053236327910792	30.959521011472486	19.090613206258027
31	23.28662539748005	29.53290526105819	27.90994084339774	19.27052849806402
32	24.101727917962318	29.9180070397315	28.295929953358318	17.684335088947865
33	23.192674760784357	30.26584090433965	27.314328590133513	19.227155744742475
34	19.708515221458367	30.199539510555162	29.527687753089065	20.564257514897406
35	19.81325580660721	29.507705052499844	30.18889153510797	20.490147605784976
36	23.090880115509236	29.750336919689772	28.195874477406242	18.96290848739475
37	20.64706327062504	31.77572382275096	28.79304843373605	18.784164472887948
38	20.208757107967276	32.280544338702164	27.130828479926972	20.379870073403595
39	20.24723179258312	31.492239223095314	28.23040941110662	20.030119573214947
40	22.312122684553373	29.149684624713878	28.198323511759092	20.339869178973654
41	18.80237251090264	28.94339784705035	29.439451529883364	22.814778112163644
42	21.20562056933305	29.428448621921273	27.573535805769573	21.792395002976107
43	21.276997498080704	28.98953907398817	28.226682619700107	21.506780808231028
44	20.02259500389893	30.884523771072786	28.54931627574991	20.54356494927837
45	19.327601646460952	32.889040642257484	26.60031084989655	21.18304686138501
46	20.727136045987894	31.6210442327548	27.726334253436725	19.925485467820575
47	20.427395537149547	30.154179135150137	28.497602608328066	20.920822719372254
48	20.784315674139297	28.605537586111062	29.948105316995555	20.662041422754086
49	20.685112036222993	28.020289362282103	29.959321184466592	21.335277417028315
50	19.515573906355314	31.29858804296245	29.082850832157025	20.102987218525207
51	18.928054114430953	31.65313013210233	27.584574206983152	21.83424154648356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2438.0
1	2770.0
2	3102.0
3	13818.5
4	24535.0
5	18642.5
6	12750.0
7	12473.5
8	12197.0
9	13618.0
10	15039.0
11	15913.0
12	16787.0
13	16960.5
14	17134.0
15	16347.5
16	15561.0
17	15518.5
18	15476.0
19	15290.5
20	15105.0
21	16650.0
22	18195.0
23	20269.5
24	22344.0
25	25896.5
26	36309.0
27	43169.0
28	50323.0
29	57477.0
30	64488.0
31	71499.0
32	81546.0
33	91593.0
34	102370.5
35	113148.0
36	118635.0
37	124122.0
38	135276.5
39	146431.0
40	169634.5
41	192838.0
42	209698.5
43	226559.0
44	233235.5
45	239912.0
46	234568.5
47	229225.0
48	221584.5
49	213944.0
50	199493.5
51	185043.0
52	170109.5
53	155176.0
54	140390.0
55	125604.0
56	114003.0
57	102402.0
58	93624.5
59	84847.0
60	77411.5
61	69976.0
62	59764.5
63	49553.0
64	40237.0
65	30921.0
66	24161.5
67	17402.0
68	13862.5
69	10323.0
70	8510.5
71	6698.0
72	5300.5
73	3903.0
74	3298.0
75	2072.5
76	1452.0
77	1067.5
78	683.0
79	512.0
80	341.0
81	261.5
82	182.0
83	131.5
84	81.0
85	63.0
86	45.0
87	38.5
88	32.0
89	31.0
90	30.0
91	23.5
92	17.0
93	9.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2817437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.716352976984414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11226249624963	15.974848487261845
2	7.693719972784212	3.1877163732454528
3	2.8316297178423917	1.759831222048253
4	1.52479279717653	1.263525832122896
5	0.9877642086602665	1.0231436002318883
6	0.7326505848591125	0.9106708874841268
7	0.5065046702359409	0.7345050673169191
8	0.43263509498813885	0.7170097070404372
9	0.36440225553961975	0.6794177176231233
>10	5.071699931400244	26.543249240455314
>50	2.2382594641085762	32.92984943829875
>100	0.5010381409274826	13.231076065693667
>500	0.0019364878337626108	0.2486522461618729
>1k	5.281330455716211E-4	0.2565297593873004
>5k	0.0	0.0
>10k+	1.760443485238737E-4	0.5399743556281447
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14806	0.5255130815702357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3652	0.12962135444377282	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.098650298125566E-5	0.0	0.0	0.024135411013626925	0.0
2	7.098650298125566E-5	0.0	0.0	0.08564521584688495	0.0
3	7.098650298125566E-5	0.0	0.0	0.13444843664649822	0.0
4	7.098650298125566E-5	0.0	0.0	0.2277956880668494	0.0
5	7.098650298125566E-5	0.0	0.0	0.4126445418300391	0.0
6	7.098650298125566E-5	0.0	0.0	0.6927927758455646	0.0
7	7.098650298125566E-5	0.0	0.0	0.8350852210714915	0.0
8	7.098650298125566E-5	0.0	0.0	1.1327671213233872	0.0
9	7.098650298125566E-5	0.0	0.0	1.2732494107232921	0.0
10	7.098650298125566E-5	0.0	0.0	1.489367819049725	0.0
11	7.098650298125566E-5	0.0	0.0	1.7769341426267917	0.0
12	7.098650298125566E-5	0.0	0.0	1.9977021668984967	0.0
13	7.098650298125566E-5	0.0	0.0	2.1176338636853282	0.0
14	7.098650298125566E-5	0.0	0.0	2.185390480780937	0.0
15	7.098650298125566E-5	0.0	0.0	2.2587550316120644	0.0
16	7.098650298125566E-5	0.0	0.0	2.3921031774623533	0.0
17	7.098650298125566E-5	0.0	0.0	2.563287129401651	0.0
18	7.098650298125566E-5	0.0	0.0	2.753779410151851	0.0
19	7.098650298125566E-5	0.0	0.0	2.8842526026313986	0.0
20	7.098650298125566E-5	0.0	0.0	3.0226052969418657	0.0
21	7.098650298125566E-5	0.0	0.0	3.196344762988489	0.0
22	7.098650298125566E-5	0.0	0.0	3.3928708964920955	0.0
23	1.0647975447188348E-4	0.0	0.0	3.6061143514477876	0.0
24	1.0647975447188348E-4	0.0	0.0	3.764769185610894	0.0
25	1.0647975447188348E-4	0.0	0.0	3.916360862727365	0.0
26	1.0647975447188348E-4	0.0	0.0	4.057482030654102	0.0
27	1.0647975447188348E-4	0.0	0.0	4.1980707998084785	0.0
28	1.0647975447188348E-4	0.0	0.0	4.3530343358165595	0.0
29	1.0647975447188348E-4	0.0	0.0	4.52212418591791	0.0
30	1.0647975447188348E-4	0.0	0.0	4.738455553753287	0.0
31	1.0647975447188348E-4	0.0	0.0	4.930474044317584	0.0
32	1.0647975447188348E-4	0.0	0.0	5.111489626919786	0.0
33	1.0647975447188348E-4	0.0	0.0	5.290695053695965	0.0
34	1.0647975447188348E-4	0.0	0.0	5.485446524625041	0.0
35	1.0647975447188348E-4	0.0	0.0	5.700784081418679	0.0
36	1.0647975447188348E-4	0.0	0.0	5.90266969589737	0.0
37	1.0647975447188348E-4	0.0	0.0	6.1092049263213335	0.0
38	1.0647975447188348E-4	0.0	0.0	6.329937457341549	0.0
39	1.0647975447188348E-4	0.0	0.0	6.606287913447576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	1350	0.0	41.166668	4
CGTTTTT	8690	0.0	41.142117	1
TTCGTAG	200	0.0	40.5	1
CGGTCTA	130	0.0	39.80769	31
CGGCTAC	515	0.0	39.32039	25
TAAGGGA	5090	0.0	39.16503	4
ATTAGCG	220	0.0	38.863636	1
ACGGGAT	1555	0.0	38.778137	5
GGGCGAT	5180	0.0	38.44112	7
CTCGTCC	445	0.0	38.426964	37
TCGTCCC	440	0.0	38.352272	38
TGGGCGA	1505	0.0	38.272423	6
ACGGGAC	1050	0.0	38.142857	5
AGGGCGA	2435	0.0	37.792606	6
TAGGGCG	835	0.0	37.724552	5
ACGCGAG	30	1.1403425E-4	37.500004	1
GCGCGTT	30	1.1403425E-4	37.500004	31
AAGGGAT	5695	0.0	37.29587	5
AGGGATC	3895	0.0	37.259308	6
CGACGGT	145	0.0	37.24138	28
>>END_MODULE