FastQCFastQC Report
Thu 26 May 2016
SRR1548002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548002_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2490450
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51090.20514364873817983No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG253.8921207E-545.0000041
GCGCGAC2000.043.8750049
CGTAAGG4700.041.6489372
TACGTCG650.041.538461
TACGGGA7850.040.1273884
CGTTTTT43200.039.6875041
GGCGATA9000.039.258
GCGATAC1150.039.1304329
AGGGCGA21750.038.896556
GCGTAAG2550.038.823531
CACGCCG2150.038.72093226
GGGCGAT43750.038.468577
ATATGCG1650.038.1818161
GACCGAT18800.038.058519
TCGATTG950.037.8947371
CGAACGG1550.037.741942
TAGGGCG8950.037.45815
CGTTAGG4400.037.3295442
TACGGGT5250.037.2857174
TAGGGAC24900.037.0481955
Produced by FastQC (version 0.11.5)