##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548002_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2490450
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89354976008352	31.0	31.0	31.0	30.0	33.0
2	31.254674456423537	31.0	31.0	33.0	30.0	34.0
3	31.47547270573591	31.0	31.0	34.0	30.0	34.0
4	35.3442289546066	37.0	35.0	37.0	33.0	37.0
5	35.160089140516774	37.0	35.0	37.0	32.0	37.0
6	35.193657371157826	37.0	35.0	37.0	32.0	37.0
7	35.53181352767572	35.0	35.0	37.0	35.0	37.0
8	35.621157622116485	37.0	35.0	37.0	35.0	37.0
9	37.47427934710594	39.0	37.0	39.0	35.0	39.0
10	36.451335702383105	38.0	35.0	39.0	32.0	39.0
11	36.20725852757534	38.0	35.0	39.0	31.0	39.0
12	35.74154470075689	37.0	35.0	39.0	30.0	39.0
13	35.4820285490574	37.0	35.0	39.0	30.0	39.0
14	36.50180288702845	38.0	35.0	40.0	30.0	41.0
15	36.71079202553755	38.0	35.0	40.0	31.0	41.0
16	36.828304924812784	38.0	35.0	40.0	31.0	41.0
17	36.77156819048766	38.0	35.0	40.0	31.0	41.0
18	36.704932441928165	38.0	35.0	40.0	31.0	41.0
19	36.664022164669035	38.0	35.0	40.0	31.0	41.0
20	36.557818065008334	38.0	35.0	40.0	31.0	41.0
21	36.44142383906523	38.0	35.0	40.0	30.0	41.0
22	36.31134413459415	38.0	35.0	40.0	30.0	41.0
23	36.24564436146078	38.0	34.0	40.0	30.0	41.0
24	36.146072798088696	38.0	34.0	40.0	30.0	41.0
25	35.96899957838945	38.0	34.0	40.0	30.0	41.0
26	35.73667610271236	38.0	34.0	40.0	29.0	41.0
27	35.82685137224196	38.0	34.0	40.0	29.0	41.0
28	35.87690618161376	38.0	34.0	40.0	29.0	41.0
29	35.90883695717641	38.0	34.0	40.0	30.0	41.0
30	35.848139091328875	38.0	34.0	40.0	29.0	41.0
31	35.77260173864161	38.0	34.0	40.0	29.0	41.0
32	35.6606123391355	38.0	34.0	40.0	29.0	41.0
33	35.56834588126644	38.0	34.0	40.0	29.0	41.0
34	35.50306008954205	38.0	34.0	40.0	28.0	41.0
35	35.38514525487362	38.0	34.0	40.0	27.0	41.0
36	35.304022967736756	38.0	34.0	40.0	27.0	41.0
37	35.26100544078379	38.0	34.0	40.0	27.0	41.0
38	35.225249252143186	38.0	34.0	40.0	27.0	41.0
39	35.2201461583248	38.0	34.0	40.0	27.0	41.0
40	35.06205866409685	38.0	34.0	40.0	26.0	41.0
41	35.005859583609386	37.0	34.0	40.0	26.0	41.0
42	34.966703206247864	37.0	34.0	40.0	26.0	41.0
43	34.93257443433917	37.0	33.0	40.0	26.0	41.0
44	34.80579252745488	37.0	33.0	40.0	26.0	41.0
45	34.6777176815435	37.0	33.0	40.0	25.0	41.0
46	34.627789756871245	37.0	33.0	40.0	25.0	41.0
47	34.58838242084764	37.0	33.0	40.0	25.0	41.0
48	34.51184283964745	37.0	33.0	40.0	24.0	41.0
49	34.39010178883334	36.0	33.0	40.0	24.0	41.0
50	34.279031500331264	36.0	33.0	40.0	24.0	41.0
51	33.95402838844385	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	9.0
14	12.0
15	73.0
16	204.0
17	515.0
18	1107.0
19	2159.0
20	3829.0
21	6087.0
22	9346.0
23	13624.0
24	19516.0
25	27486.0
26	35741.0
27	43331.0
28	51277.0
29	62178.0
30	76214.0
31	94370.0
32	115788.0
33	146591.0
34	216783.0
35	257933.0
36	212382.0
37	263224.0
38	369282.0
39	461342.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.78758858840772	25.668453492340742	30.510188921680818	12.033768997570721
2	28.343472063281737	27.146218554879642	30.480234495773857	14.030074886064767
3	28.557248689995784	26.656026019394087	31.45343211066273	13.3332931799474
4	25.22993836455259	29.74631090766729	30.081310606516894	14.942440121263227
5	23.370314601778794	33.18147322772993	29.138709871709935	14.309502298781346
6	20.506936497420146	43.34140416390612	25.478206749784178	10.673452588889559
7	92.71091569796623	2.875745347226405	3.5249452910116643	0.8883936637956996
8	93.8844787086671	1.9377220984159491	3.12373265875645	1.0540665341604931
9	89.72723804934851	3.74735489570158	4.85538758055773	1.670019474392178
10	37.37706037061575	29.619867895360276	19.385813808749422	13.617257925274547
11	34.43907727519123	26.928948583589314	21.9770322632456	16.65494187797386
12	32.860366600413585	25.294223935433358	23.696239635407256	18.14916982874581
13	23.725872834226745	31.293902708345883	25.604448995161516	19.375775462265857
14	19.307032865546386	33.63191391114056	27.394366479953426	19.666686743359634
15	18.706699592443133	27.315545383364455	36.24276737135859	17.734987652833826
16	22.024895099279245	25.189343291373046	35.0585235599992	17.72723804934851
17	21.587785339998796	24.933726836515486	27.706478748820494	25.77200907466522
18	23.452508582786244	26.3546748579574	30.31604730068863	19.876769258567727
19	26.466983878415544	27.59585617057158	25.94025176172981	19.99690818928306
20	28.784516854383746	27.158385030817723	25.925073781846653	18.132024332951875
21	24.88200927543215	28.800497901985583	27.724387158947177	18.593105663635086
22	25.04029392278504	24.792708145114336	27.294786082836435	22.87221184926419
23	22.036941115059527	29.086470316609446	27.085787709048564	21.79080085928246
24	21.483185769640027	26.45015960970909	32.997651026922846	19.06900359372804
25	20.880523600152586	27.904113714388966	31.027163765584532	20.18819891987392
26	21.29719528599249	32.47473348190086	26.183300206789937	20.04477102531671
27	20.3348792386918	31.784336164147042	28.462366239033106	19.418418358128047
28	18.03320685016764	30.55114537533378	32.639201750687626	18.77644602381096
29	19.73691501535867	27.632435905157703	31.918609086711236	20.71203999277239
30	20.67084261880383	29.612158445260896	29.66411692665984	20.052882009275432
31	25.26294444779056	28.04854544359453	27.09409945993696	19.594410648677947
32	25.63781645887289	28.15402838844386	27.21138750025096	18.996767652432293
33	24.477624525688128	28.639000983757956	26.71159830552711	20.1717761850268
34	20.477785139231866	29.015438976891726	28.843863558794595	21.662912325081813
35	21.208897990323035	28.27814250436668	29.185127185849947	21.327832319460338
36	24.76030436266538	28.200044168724524	27.136059748238274	19.90359172037182
37	21.125539561123492	30.316569294705776	28.76825473308037	19.789636411090363
38	20.898632777208938	30.743721014274527	26.80800658515529	21.549639623361237
39	21.206167560079503	30.03742295569074	27.63865967997751	21.117749804252245
40	23.461262020919914	27.697645003914957	26.757252705334377	22.083840269830755
41	19.248449075468287	27.10839406532956	28.7712260836395	24.87193077556265
42	21.716236021602523	27.938163785661224	26.80776566483969	23.537834527896564
43	21.44993876608645	27.721455961773977	27.82870565560441	22.999899616535163
44	21.062378285048887	28.939508924090024	28.183581280491477	21.814531510369612
45	19.916601417414523	31.675359874721437	25.83721817342247	22.570820534441566
46	21.466481961091368	30.452127125619867	26.833222911522014	21.24816800176675
47	21.283422674617036	28.342267461703706	28.016623501776788	22.357686361902466
48	22.14298620731193	26.691280692244373	29.924511634443572	21.24122146600012
49	21.534903330723363	26.411050211809112	30.01883193800317	22.035214519464354
50	20.37463109076673	30.458511513983417	27.939930534642333	21.22692686060752
51	19.555542171093578	30.30809693027365	27.258447268565924	22.877913630066857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1368.0
1	1734.5
2	2101.0
3	6310.5
4	10520.0
5	8466.0
6	6412.0
7	6323.0
8	6234.0
9	6648.0
10	7062.0
11	7218.5
12	7375.0
13	7418.5
14	7462.0
15	7291.5
16	7121.0
17	7173.5
18	7226.0
19	8057.0
20	8888.0
21	9965.0
22	11042.0
23	12604.0
24	14166.0
25	16571.0
26	22913.5
27	26851.0
28	33254.0
29	39657.0
30	45970.5
31	52284.0
32	62235.0
33	72186.0
34	80676.0
35	89166.0
36	93888.5
37	98611.0
38	111320.0
39	124029.0
40	145659.0
41	167289.0
42	185668.0
43	204047.0
44	206074.5
45	208102.0
46	208983.5
47	209865.0
48	203752.0
49	197639.0
50	189737.0
51	181835.0
52	172465.5
53	163096.0
54	146563.0
55	130030.0
56	117673.5
57	105317.0
58	97424.0
59	89531.0
60	80157.0
61	70783.0
62	61722.5
63	52662.0
64	43212.5
65	33763.0
66	27813.0
67	21863.0
68	18013.5
69	14164.0
70	11122.5
71	8081.0
72	6492.5
73	4904.0
74	4119.5
75	3141.5
76	2948.0
77	2097.5
78	1247.0
79	943.5
80	640.0
81	425.0
82	210.0
83	205.5
84	201.0
85	137.0
86	73.0
87	59.0
88	45.0
89	30.0
90	15.0
91	12.5
92	10.0
93	7.5
94	5.0
95	8.5
96	12.0
97	6.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2490450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.500276238096678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01284218654894	14.432591305701973
2	7.18649546003763	2.6590430238904763
3	2.22659971460753	1.235781293757196
4	1.1250264704260209	0.8325320191220915
5	0.7519279030693813	0.695543695895817
6	0.5581525835098845	0.6195586186764113
7	0.44025027256002225	0.5701326159380435
8	0.36991563465921623	0.547483314078908
9	0.34384314977643543	0.5725073928097174
>10	5.681814509718055	27.712276543196555
>50	2.734268126748639	35.97925748418527
>100	0.5659752275054314	13.515215600662959
>500	0.0019999113458282185	0.2358064013743107
>1k	6.666371152760727E-4	0.18224019827271953
>5k	2.2221237175869095E-4	0.2100304924374744
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5109	0.20514364873817983	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.030677186853781E-5	0.0	0.0	0.016221967917444638	0.0
2	8.030677186853781E-5	0.0	0.0	0.06243851512778815	0.0
3	8.030677186853781E-5	0.0	0.0	0.09994177759039531	0.0
4	8.030677186853781E-5	0.0	0.0	0.1881587664879841	0.0
5	8.030677186853781E-5	0.0	0.0	0.3302615993093618	0.0
6	8.030677186853781E-5	0.0	0.0	0.5579312975566665	0.0
7	8.030677186853781E-5	0.0	0.0	0.6556244855347427	0.0
8	8.030677186853781E-5	0.0	0.0	0.88755044269108	0.0
9	8.030677186853781E-5	0.0	0.0	0.9670541468409324	0.0
10	8.030677186853781E-5	0.0	0.0	1.134252845871228	0.0
11	8.030677186853781E-5	0.0	0.0	1.380031721174888	0.0
12	8.030677186853781E-5	0.0	0.0	1.5490774759581603	0.0
13	8.030677186853781E-5	0.0	0.0	1.6427553253428095	0.0
14	8.030677186853781E-5	0.0	0.0	1.6896143267281014	0.0
15	8.030677186853781E-5	0.0	0.0	1.7481981168061997	0.0
16	8.030677186853781E-5	0.0	0.0	1.882029352125118	0.0
17	8.030677186853781E-5	0.0	0.0	2.0336886907988516	0.0
18	8.030677186853781E-5	0.0	0.0	2.2057057961412596	0.0
19	8.030677186853781E-5	0.0	0.0	2.306691561765946	0.0
20	8.030677186853781E-5	0.0	0.0	2.414061715754181	0.0
21	8.030677186853781E-5	0.0	0.0	2.560380654098657	0.0
22	8.030677186853781E-5	0.0	0.0	2.7186251480656107	0.0
23	8.030677186853781E-5	0.0	0.0	2.8916460880563752	0.0
24	8.030677186853781E-5	0.0	0.0	3.0299343492139976	0.0
25	8.030677186853781E-5	0.0	0.0	3.161717761850268	0.0
26	8.030677186853781E-5	0.0	0.0	3.281174085004718	0.0
27	8.030677186853781E-5	0.0	0.0	3.3987833524061917	0.0
28	8.030677186853781E-5	0.0	0.0	3.5328153546547814	0.0
29	8.030677186853781E-5	0.0	0.0	3.67182637675922	0.0
30	8.030677186853781E-5	0.0	0.0	3.848782348571543	0.0
31	8.030677186853781E-5	0.0	0.0	4.008552671203999	0.0
32	8.030677186853781E-5	0.0	0.0	4.171976951956474	0.0
33	8.030677186853781E-5	0.0	0.0	4.328494850328254	0.0
34	8.030677186853781E-5	0.0	0.0	4.489228854223132	0.0
35	8.030677186853781E-5	0.0	0.0	4.673532895661427	0.0
36	8.030677186853781E-5	0.0	0.0	4.844465859583609	0.0
37	8.030677186853781E-5	0.0	0.0	5.017808026661848	0.0
38	8.030677186853781E-5	0.0	0.0	5.2074524684294	0.0
39	8.030677186853781E-5	0.0	0.0	5.442189162601136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	25	3.8921207E-5	45.000004	1
GCGCGAC	200	0.0	43.875004	9
CGTAAGG	470	0.0	41.648937	2
TACGTCG	65	0.0	41.53846	1
TACGGGA	785	0.0	40.127388	4
CGTTTTT	4320	0.0	39.687504	1
GGCGATA	900	0.0	39.25	8
GCGATAC	115	0.0	39.130432	9
AGGGCGA	2175	0.0	38.89655	6
GCGTAAG	255	0.0	38.82353	1
CACGCCG	215	0.0	38.720932	26
GGGCGAT	4375	0.0	38.46857	7
ATATGCG	165	0.0	38.181816	1
GACCGAT	1880	0.0	38.05851	9
TCGATTG	95	0.0	37.894737	1
CGAACGG	155	0.0	37.74194	2
TAGGGCG	895	0.0	37.4581	5
CGTTAGG	440	0.0	37.329544	2
TACGGGT	525	0.0	37.285717	4
TAGGGAC	2490	0.0	37.048195	5
>>END_MODULE