FastQCFastQC Report
Thu 26 May 2016
SRR1548001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548001_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1758962
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256831.46012250406774No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.13957095150435314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.0343085E-445.01
ACGTTCG302.1661617E-644.99999613
CGTTTTT139850.043.262431
TACGGGA8900.041.7134864
CGTTAGG2150.040.8139532
GCGATAC950.040.263169
GAGTACG451.9292202E-840.01
AGGGCGA15750.039.571436
TATAGCG1150.039.1304361
ACGGGAT11100.039.121625
CGTTCGA356.2501804E-638.57142614
CGACGGT659.094947E-1238.07692728
AAGGGAT39250.038.0636945
CGGTTTT12700.037.9133871
TAGGGAT38550.037.7626465
GGCACCG7450.037.751688
TAGGGAC23350.037.5803035
AGGGACC21300.037.56
TAACGCG301.1401682E-437.4999961
TATGGGA24750.037.4545444
Produced by FastQC (version 0.11.5)