FastQCFastQC Report
Thu 26 May 2016
SRR1548000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548000_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1560871
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.24018640874229838No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22410.14357368418017888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG406.8175723E-945.0000041
GCGTAAG1250.041.41
AATGCGG2150.040.8139532
GCGAGAC1850.040.13513621
TACGGGA7800.040.0961534
CGACCCG1750.039.85714332
GCTTGCG1200.039.3749961
AGGGCGA14100.038.7765966
ACGGGTA1050.038.571435
CGAGGGA12800.038.3203164
GCACCGA7050.038.2978749
CGAACGG1000.038.252
CGACGGT1300.038.07692728
CGTTTTT26700.037.921351
TTCGTAG950.037.8947371
TAGGGAT31100.037.837625
ATAGGGA26350.037.6565484
CGCATCG900.037.50000421
TAGCGGG9900.037.53
CACGACC2050.037.31707427